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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR8093Btlr/Mmmh
Stock Number:
067525-MU
Citation ID:
RRID:MMRRC_067525-MU
Other Names:
R8093 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Atrn
Name: attractin
Synonyms: Mgca
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11990
HGNC: HGNC:885
Homologene: 22542

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Tnf
Name: tumor necrosis factor
Synonyms: TNF-alpha, tumor necrosis factor-alpha, TNF alpha, TNFalpha, DIF, Tnfsf1a, Tnfa
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 21926
Homologene: 496

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IMPC
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Gene Ontology
BioGPS
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
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ClinGen
Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441

Additional Resources
IMPC
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Gene Ontology
BioGPS
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Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Map2k6
Name: mitogen-activated protein kinase kinase 6
Synonyms: MAP kinase kinase 6, MKK6, SAPKK3, Prkmk6
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 26399
HGNC: HGNC:6846
Homologene: 55686

Additional Resources
IMPC
Google
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Gene Ontology
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Mouse Mine
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Arfgef2
Name: ARF guanine nucleotide exchange factor 2
Synonyms: BIG2, E230011G24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99371
Homologene: 111880

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Dnajc11
Name: DnaJ heat shock protein family (Hsp40) member C11
Synonyms: E030019A03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230935
Homologene: 14558

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Etaa1
Name: Ewing tumor-associated antigen 1
Synonyms: 5730466H23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68145
Homologene: 10369

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 36,227,485 bp
  • T to C, chromosome 1 at 38,075,016 bp
  • C to T, chromosome 1 at 51,757,875 bp
  • T to C, chromosome 1 at 71,280,393 bp
  • T to A, chromosome 1 at 88,166,582 bp
  • C to A, chromosome 1 at 93,274,665 bp
  • C to A, chromosome 1 at 97,885,632 bp
  • T to A, chromosome 1 at 133,360,074 bp
  • G to T, chromosome 1 at 151,210,304 bp
  • T to C, chromosome 2 at 121,454,953 bp
  • T to C, chromosome 2 at 130,975,988 bp
  • A to G, chromosome 2 at 166,894,657 bp
  • T to C, chromosome 3 at 85,672,804 bp
  • T to C, chromosome 3 at 101,056,437 bp
  • C to T, chromosome 3 at 101,072,941 bp
  • T to G, chromosome 3 at 158,051,782 bp
  • A to T, chromosome 4 at 73,403,522 bp
  • G to T, chromosome 4 at 120,095,435 bp
  • A to G, chromosome 4 at 148,270,516 bp
  • A to T, chromosome 4 at 151,969,900 bp
  • T to C, chromosome 5 at 6,770,082 bp
  • T to A, chromosome 5 at 15,525,816 bp
  • T to C, chromosome 5 at 114,955,277 bp
  • T to C, chromosome 5 at 120,459,952 bp
  • T to A, chromosome 5 at 123,501,129 bp
  • C to A, chromosome 5 at 124,556,820 bp
  • A to G, chromosome 5 at 128,602,239 bp
  • A to G, chromosome 6 at 48,443,432 bp
  • A to G, chromosome 6 at 51,481,768 bp
  • A to G, chromosome 6 at 70,074,563 bp
  • T to G, chromosome 6 at 73,160,913 bp
  • G to T, chromosome 6 at 86,721,426 bp
  • T to A, chromosome 6 at 100,733,227 bp
  • T to C, chromosome 7 at 21,300,591 bp
  • A to G, chromosome 7 at 27,036,629 bp
  • T to A, chromosome 7 at 27,281,036 bp
  • G to T, chromosome 7 at 78,843,509 bp
  • A to G, chromosome 7 at 143,362,652 bp
  • T to A, chromosome 8 at 3,508,740 bp
  • A to G, chromosome 8 at 57,532,705 bp
  • G to T, chromosome 8 at 122,866,835 bp
  • A to T, chromosome 9 at 44,356,232 bp
  • G to T, chromosome 9 at 48,396,552 bp
  • T to C, chromosome 10 at 127,750,914 bp
  • G to A, chromosome 11 at 17,947,559 bp
  • A to G, chromosome 11 at 67,188,710 bp
  • A to G, chromosome 11 at 95,749,462 bp
  • A to C, chromosome 11 at 110,482,585 bp
  • C to T, chromosome 12 at 81,918,819 bp
  • G to A, chromosome 12 at 91,743,686 bp
  • A to G, chromosome 12 at 105,809,577 bp
  • T to C, chromosome 13 at 13,359,406 bp
  • T to C, chromosome 13 at 62,369,549 bp
  • T to A, chromosome 13 at 74,407,019 bp
  • T to C, chromosome 14 at 8,028,353 bp
  • A to G, chromosome 14 at 51,456,242 bp
  • G to A, chromosome 14 at 52,667,669 bp
  • A to T, chromosome 14 at 53,461,683 bp
  • T to A, chromosome 15 at 42,476,477 bp
  • T to A, chromosome 15 at 85,882,720 bp
  • T to A, chromosome 15 at 100,424,012 bp
  • A to T, chromosome 16 at 4,851,504 bp
  • G to A, chromosome 16 at 34,709,652 bp
  • T to C, chromosome 17 at 35,201,096 bp
  • A to G, chromosome 18 at 41,932,635 bp
  • A to G, chromosome 18 at 57,879,351 bp
  • T to A, chromosome 19 at 6,386,042 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8093 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
067525-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.