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Strain Name:
C57BL/6J-MtgxR8097Btlr/Mmmh
Stock Number:
067529-MU
Citation ID:
RRID:MMRRC_067529-MU
Other Names:
R8097 (G1)
Major Collection:

Strain Information

Ubc
Name: ubiquitin C
Synonyms: 2700054O04Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22190
Homologene: 128418
Kcna6
Name: potassium voltage-gated channel, shaker-related, subfamily, member 6
Synonyms: Kv1.6, MK1.6
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16494
HGNC: HGNC:6225
Homologene: 1684
Ctnnal1
Name: catenin alpha like 1
Synonyms: ACRP, Catnal1, catenin (cadherin associated protein), alpha-like 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 54366
HGNC: HGNC:2512
Homologene: 2815
Col18a1
Name: collagen, type XVIII, alpha 1
Synonyms: endostatin
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12822
HGNC: HGNC:2195
Homologene: 7673
Lhx9
Name: LIM homeobox protein 9
Synonyms: LH2B, Lhx9 alpha, 3110009O07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16876
Homologene: 7816
Etf1
Name: eukaryotic translation termination factor 1
Synonyms: SUP45L1, TB3-1, ERF1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225363
HGNC: HGNC:3477
Homologene: 3475
Zfp516
Name: zinc finger protein 516
Synonyms: C330029B10Rik, Zfp26l
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 329003
VEGA: 18
Homologene: 37122
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 67,228,270 bp
  • T to C, chromosome 1 at 138,838,351 bp
  • C to T, chromosome 1 at 185,329,389 bp
  • A to T, chromosome 2 at 86,217,666 bp
  • A to T, chromosome 2 at 89,193,632 bp
  • T to C, chromosome 2 at 112,670,270 bp
  • TCTCCTC to TCTC, chromosome 2 at 121,439,456 bp
  • A to G, chromosome 2 at 126,754,880 bp
  • T to C, chromosome 2 at 129,463,585 bp
  • A to T, chromosome 3 at 89,409,135 bp
  • C to T, chromosome 3 at 96,850,240 bp
  • C to T, chromosome 3 at 153,877,908 bp
  • T to C, chromosome 4 at 56,847,845 bp
  • A to T, chromosome 4 at 88,557,701 bp
  • G to A, chromosome 4 at 96,215,410 bp
  • G to A, chromosome 4 at 130,007,897 bp
  • G to T, chromosome 4 at 137,728,286 bp
  • T to A, chromosome 5 at 52,795,991 bp
  • C to T, chromosome 5 at 121,749,223 bp
  • C to T, chromosome 5 at 125,389,918 bp
  • A to T, chromosome 6 at 125,168,982 bp
  • A to T, chromosome 6 at 126,738,612 bp
  • A to T, chromosome 6 at 142,374,911 bp
  • G to A, chromosome 6 at 149,169,355 bp
  • A to T, chromosome 7 at 5,327,651 bp
  • A to T, chromosome 7 at 14,082,710 bp
  • A to T, chromosome 7 at 17,975,530 bp
  • A to G, chromosome 7 at 49,587,777 bp
  • A to G, chromosome 7 at 102,713,990 bp
  • C to T, chromosome 7 at 127,393,324 bp
  • C to T, chromosome 7 at 129,607,887 bp
  • A to G, chromosome 7 at 141,195,906 bp
  • A to G, chromosome 8 at 57,962,373 bp
  • T to A, chromosome 9 at 57,679,553 bp
  • T to A, chromosome 9 at 60,615,048 bp
  • T to C, chromosome 9 at 64,924,452 bp
  • T to C, chromosome 9 at 76,015,139 bp
  • T to C, chromosome 9 at 79,818,259 bp
  • T to C, chromosome 9 at 108,463,552 bp
  • C to A, chromosome 9 at 111,256,092 bp
  • G to A, chromosome 10 at 27,190,664 bp
  • T to C, chromosome 10 at 41,634,119 bp
  • A to C, chromosome 10 at 77,112,508 bp
  • T to C, chromosome 10 at 86,869,095 bp
  • A to G, chromosome 10 at 96,026,654 bp
  • T to A, chromosome 11 at 103,504,099 bp
  • C to T, chromosome 12 at 30,006,602 bp
  • A to G, chromosome 12 at 73,043,750 bp
  • T to C, chromosome 13 at 11,945,995 bp
  • T to A, chromosome 13 at 49,790,200 bp
  • T to C, chromosome 14 at 19,798,589 bp
  • T to A, chromosome 14 at 23,330,964 bp
  • T to C, chromosome 14 at 24,306,852 bp
  • T to C, chromosome 15 at 35,709,346 bp
  • A to T, chromosome 15 at 64,871,862 bp
  • CAGCTGGAGGAGC to CAGC, chromosome 15 at 78,436,913 bp
  • C to A, chromosome 15 at 82,390,380 bp
  • C to A, chromosome 17 at 17,970,792 bp
  • A to G, chromosome 17 at 20,502,349 bp
  • A to T, chromosome 17 at 37,279,927 bp
  • T to A, chromosome 17 at 74,412,698 bp
  • T to C, chromosome 18 at 34,931,644 bp
  • C to T, chromosome 18 at 71,679,502 bp
  • C to A, chromosome 18 at 82,987,170 bp
  • G to T, chromosome 19 at 38,161,966 bp
  • G to A, chromosome 19 at 43,816,955 bp
  • A to G, chromosome 19 at 47,257,519 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8097 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067529-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.