Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR8101Btlr/Mmmh
Stock Number:
067533-MU
Citation ID:
RRID:MMRRC_067533-MU
Other Names:
R8101 (G1)
Major Collection:

Strain Information

Mtrr
Name: 5-methyltetrahydrofolate-homocysteine methyltransferase reductase
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 210009
VEGA: 13
HGNC: HGNC:7473
Homologene: 11419
Myh7
Name: myosin, heavy polypeptide 7, cardiac muscle, beta
Synonyms: Myhc-b, Myhcb, beta-MHC, B-MHC, MYH-beta/slow, MyHC-I, betaMHC
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 140781
HGNC: HGNC:7577
Homologene: 68044
Dock1
Name: dedicator of cytokinesis 1
Synonyms: D630004B07Rik, 9130006G06Rik, Dock180, b2b3190Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330662
HGNC: HGNC:2987
Homologene: 55575
Ddx10
Name: DEAD box helicase 10
Synonyms: 4632415A01Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 77591
VEGA: 9
HGNC: HGNC:2735
Homologene: 20922
Gpr139
Name: G protein-coupled receptor 139
Synonyms: LOC209776, GPRg1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 209776
Homologene: 45860
Ltn1
Name: listerin E3 ubiquitin protein ligase 1
Synonyms: 4930528H02Rik, Zfp294, Listerin, Rnf160
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 78913
VEGA: 16
Homologene: 32272
Arhgap35
Name: Rho GTPase activating protein 35
Synonyms: P190 RhoGAP, 6430596G11Rik, p190RhoGAP, p190A, Grlf1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232906
HGNC: HGNC:4591
Homologene: 35136
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 4,912,415 bp
  • T to C, chromosome 1 at 43,970,440 bp
  • C to T, chromosome 1 at 136,118,665 bp
  • C to T, chromosome 1 at 136,476,352 bp
  • C to A, chromosome 2 at 31,350,070 bp
  • T to C, chromosome 2 at 160,781,699 bp
  • A to G, chromosome 2 at 168,231,881 bp
  • A to G, chromosome 3 at 37,008,502 bp
  • A to G, chromosome 3 at 154,733,513 bp
  • A to G, chromosome 4 at 43,239,918 bp
  • A to G, chromosome 4 at 52,825,849 bp
  • G to A, chromosome 4 at 150,617,339 bp
  • A to G, chromosome 5 at 36,748,141 bp
  • A to G, chromosome 5 at 86,664,962 bp
  • A to G, chromosome 5 at 88,753,234 bp
  • A to G, chromosome 6 at 30,098,450 bp
  • C to T, chromosome 6 at 125,570,559 bp
  • T to A, chromosome 6 at 126,891,649 bp
  • A to T, chromosome 7 at 16,562,319 bp
  • T to A, chromosome 7 at 64,372,486 bp
  • T to C, chromosome 7 at 66,342,782 bp
  • A to G, chromosome 7 at 119,184,287 bp
  • A to G, chromosome 7 at 134,999,288 bp
  • A to G, chromosome 7 at 141,865,175 bp
  • T to C, chromosome 8 at 14,831,600 bp
  • G to T, chromosome 8 at 27,200,302 bp
  • A to G, chromosome 8 at 124,028,040 bp
  • A to C, chromosome 9 at 7,446,985 bp
  • A to C, chromosome 9 at 7,861,004 bp
  • T to C, chromosome 9 at 53,225,520 bp
  • A to T, chromosome 9 at 123,500,151 bp
  • T to C, chromosome 10 at 23,822,943 bp
  • T to C, chromosome 10 at 75,818,514 bp
  • T to C, chromosome 10 at 92,884,796 bp
  • A to G, chromosome 10 at 130,075,006 bp
  • T to A, chromosome 11 at 118,125,918 bp
  • C to T, chromosome 12 at 111,980,052 bp
  • A to T, chromosome 13 at 46,428,968 bp
  • C to T, chromosome 13 at 46,429,291 bp
  • A to G, chromosome 13 at 68,577,621 bp
  • C to T, chromosome 13 at 113,320,891 bp
  • A to G, chromosome 14 at 27,442,481 bp
  • G to A, chromosome 14 at 54,973,319 bp
  • G to A, chromosome 14 at 70,567,842 bp
  • G to A, chromosome 15 at 81,338,047 bp
  • A to T, chromosome 15 at 98,186,958 bp
  • A to G, chromosome 16 at 11,571,716 bp
  • G to T, chromosome 16 at 18,218,012 bp
  • A to T, chromosome 16 at 66,812,842 bp
  • A to G, chromosome 16 at 72,978,581 bp
  • G to A, chromosome 16 at 87,418,497 bp
  • A to T, chromosome 17 at 67,745,922 bp
  • T to C, chromosome 18 at 35,653,233 bp
  • A to T, chromosome 19 at 3,978,134 bp
  • C to T, chromosome 19 at 5,476,218 bp
  • T to C, chromosome 19 at 23,334,029 bp
  • T to C, chromosome 19 at 33,720,994 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8101 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067533-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.