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Strain Name:
C57BL/6J-MtgxR8103Btlr/Mmmh
Stock Number:
067534-MU
Citation ID:
RRID:MMRRC_067534-MU
Other Names:
R8103 (G1)
Major Collection:

Strain Information

Htr3b
Name: 5-hydroxytryptamine (serotonin) receptor 3B
Synonyms: 5-HT3 receptor subunit B, 5-HT3B
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 57014
VEGA: 9
HGNC: HGNC:5298
Homologene: 38131
Vcan
Name: versican
Synonyms: PG-M, hdf, heart defect, 5430420N07Rik, Cspg2, DPEAAE
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
Psen2
Name: presenilin 2
Synonyms: ALG-3, PS-2, PS2, Ad4h
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19165
HGNC: HGNC:9509
Homologene: 386
Ece1
Name: endothelin converting enzyme 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230857
HGNC: HGNC:3146
Homologene: 1068
Sptan1
Name: spectrin alpha, non-erythrocytic 1
Synonyms: alpha-fodrin, Spna-2, 2610027H02Rik, Spna2, alphaII-spectrin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20740
Homologene: 2353
Zfp553
Name: zinc finger protein 553
Synonyms: C330013F15Rik, 2600009K23Rik, ENSMUSG00000054461
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233887
Homologene: 27098
Zfp523
Name: zinc finger protein 523
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224656
Homologene: 2569
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 20,200,757 bp
  • T to C, chromosome 1 at 21,479,396 bp
  • A to T, chromosome 1 at 90,059,608 bp
  • A to G, chromosome 1 at 118,304,707 bp
  • G to T, chromosome 1 at 170,302,715 bp
  • A to T, chromosome 1 at 180,240,791 bp
  • C to A, chromosome 2 at 5,040,202 bp
  • T to A, chromosome 2 at 18,047,738 bp
  • T to A, chromosome 2 at 30,020,043 bp
  • T to C, chromosome 2 at 74,522,255 bp
  • C to T, chromosome 2 at 122,287,054 bp
  • A to T, chromosome 2 at 174,111,209 bp
  • A to G, chromosome 2 at 178,468,213 bp
  • A to G, chromosome 3 at 96,679,681 bp
  • T to A, chromosome 4 at 59,365,916 bp
  • T to A, chromosome 4 at 118,387,864 bp
  • C to A, chromosome 4 at 132,204,778 bp
  • T to C, chromosome 4 at 134,507,510 bp
  • T to C, chromosome 4 at 137,913,822 bp
  • T to A, chromosome 4 at 148,496,561 bp
  • G to A, chromosome 4 at 150,617,339 bp
  • T to C, chromosome 4 at 155,888,230 bp
  • A to T, chromosome 5 at 93,161,148 bp
  • T to A, chromosome 5 at 151,046,970 bp
  • A to T, chromosome 6 at 8,427,121 bp
  • T to C, chromosome 6 at 87,188,216 bp
  • C to T, chromosome 6 at 88,618,681 bp
  • A to G, chromosome 6 at 106,715,650 bp
  • G to A, chromosome 6 at 108,852,563 bp
  • T to C, chromosome 6 at 115,473,141 bp
  • C to T, chromosome 6 at 116,551,507 bp
  • A to G, chromosome 7 at 84,946,711 bp
  • A to G, chromosome 7 at 114,234,967 bp
  • T to C, chromosome 7 at 118,743,632 bp
  • T to C, chromosome 7 at 127,236,764 bp
  • C to T, chromosome 7 at 141,064,651 bp
  • C to A, chromosome 8 at 70,381,286 bp
  • A to G, chromosome 8 at 83,724,476 bp
  • T to C, chromosome 8 at 105,772,547 bp
  • T to A, chromosome 9 at 26,765,684 bp
  • T to C, chromosome 9 at 48,392,720 bp
  • A to G, chromosome 9 at 48,946,549 bp
  • A to G, chromosome 9 at 108,975,384 bp
  • A to T, chromosome 9 at 123,829,388 bp
  • T to G, chromosome 10 at 53,299,324 bp
  • T to C, chromosome 10 at 79,067,834 bp
  • T to A, chromosome 10 at 94,871,082 bp
  • C to A, chromosome 10 at 100,577,248 bp
  • A to G, chromosome 10 at 119,978,535 bp
  • T to C, chromosome 11 at 7,198,106 bp
  • T to A, chromosome 11 at 51,570,187 bp
  • T to C, chromosome 11 at 68,992,293 bp
  • T to C, chromosome 11 at 70,014,342 bp
  • C to T, chromosome 11 at 73,847,909 bp
  • T to C, chromosome 11 at 108,931,543 bp
  • T to A, chromosome 11 at 115,416,025 bp
  • A to G, chromosome 12 at 38,136,581 bp
  • A to G, chromosome 13 at 21,982,374 bp
  • A to G, chromosome 13 at 37,941,701 bp
  • T to A, chromosome 13 at 60,749,195 bp
  • T to A, chromosome 13 at 89,657,658 bp
  • C to A, chromosome 13 at 89,703,320 bp
  • G to A, chromosome 13 at 100,301,343 bp
  • T to C, chromosome 14 at 36,896,283 bp
  • A to G, chromosome 15 at 7,977,131 bp
  • A to G, chromosome 15 at 58,053,266 bp
  • T to A, chromosome 15 at 75,709,266 bp
  • G to T, chromosome 15 at 77,099,454 bp
  • C to A, chromosome 17 at 19,531,153 bp
  • A to G, chromosome 17 at 20,182,577 bp
  • A to G, chromosome 17 at 25,777,421 bp
  • T to C, chromosome 17 at 28,201,293 bp
  • T to G, chromosome 17 at 34,653,975 bp
  • A to T, chromosome 17 at 38,055,012 bp
  • A to C, chromosome 17 at 46,891,120 bp
  • A to T, chromosome 17 at 84,658,528 bp
  • T to G, chromosome 18 at 20,283,114 bp
  • T to C, chromosome 18 at 21,089,385 bp
  • A to G, chromosome 18 at 34,607,178 bp
  • T to A, chromosome 18 at 38,282,159 bp
  • A to G, chromosome 18 at 39,371,124 bp
  • A to G, chromosome 19 at 3,815,381 bp
  • C to T, chromosome Y at 3,776,675 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8103 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067534-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.