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Strain Name:
C57BL/6J-MtgxR8141Btlr/Mmmh
Stock Number:
067569-MU
Citation ID:
RRID:MMRRC_067569-MU
Other Names:
R8141 (G1)
Major Collection:

Strain Information

Plcb4
Name: phospholipase C, beta 4
Synonyms: C230058B11Rik, A930039J07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18798
HGNC: HGNC:9059
Homologene: 8471
Usp47
Name: ubiquitin specific peptidase 47
Synonyms: 4930502N04Rik, A630020C16Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74996
Homologene: 9929
Sf3b3
Name: splicing factor 3b, subunit 3
Synonyms: 5730409A01Rik, 1810061H24Rik, SAP130, RSE1, D8Ertd633e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 101943
Homologene: 6579
Rasal2
Name: RAS protein activator like 2
Synonyms: A330066M24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226525
HGNC: HGNC:9874
Homologene: 35217
Kdm3b
Name: KDM3B lysine (K)-specific demethylase 3B
Synonyms: 5830462I21Rik, JHDM2B, Jmjd1b
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 277250
VEGA: 18
HGNC: HGNC:1337
Homologene: 41145
Casr
Name: calcium-sensing receptor
Synonyms: CaR, Gprc2a, cation sensing receptor
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12374
HGNC: HGNC:1514
Homologene: 332
Cpne6
Name: copine VI
Synonyms: neuronal copine
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 12891
HGNC: HGNC:2319
Homologene: 81815
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 157,164,670 bp
  • A to G, chromosome 1 at 173,755,623 bp
  • T to C, chromosome 1 at 174,069,922 bp
  • A to G, chromosome 1 at 180,974,211 bp
  • T to C, chromosome 2 at 4,752,825 bp
  • C to T, chromosome 2 at 29,067,226 bp
  • T to C, chromosome 2 at 135,939,249 bp
  • T to C, chromosome 2 at 153,070,871 bp
  • T to C, chromosome 3 at 32,894,876 bp
  • A to G, chromosome 3 at 59,179,599 bp
  • A to G, chromosome 3 at 59,330,528 bp
  • C to T, chromosome 3 at 87,086,428 bp
  • C to A, chromosome 3 at 94,488,543 bp
  • G to T, chromosome 3 at 102,935,569 bp
  • A to G, chromosome 3 at 103,886,327 bp
  • A to C, chromosome 4 at 129,929,704 bp
  • C to T, chromosome 4 at 134,936,871 bp
  • T to C, chromosome 4 at 144,194,207 bp
  • T to C, chromosome 5 at 75,177,726 bp
  • T to G, chromosome 5 at 123,286,430 bp
  • CTCCAGCTCCGAT to C, chromosome 5 at 134,729,149 bp
  • A to G, chromosome 5 at 142,453,961 bp
  • T to A, chromosome 6 at 24,491,609 bp
  • A to T, chromosome 6 at 124,531,362 bp
  • T to C, chromosome 6 at 129,872,329 bp
  • A to T, chromosome 7 at 24,864,829 bp
  • G to A, chromosome 7 at 27,129,253 bp
  • A to G, chromosome 7 at 112,053,265 bp
  • A to C, chromosome 7 at 132,760,029 bp
  • T to C, chromosome 8 at 3,521,384 bp
  • A to C, chromosome 8 at 110,820,851 bp
  • T to A, chromosome 9 at 15,997,066 bp
  • T to C, chromosome 9 at 57,258,405 bp
  • G to A, chromosome 9 at 107,996,792 bp
  • A to G, chromosome 11 at 58,286,585 bp
  • G to C, chromosome 11 at 60,710,316 bp
  • T to C, chromosome 11 at 103,621,029 bp
  • A to G, chromosome 12 at 70,030,021 bp
  • A to G, chromosome 12 at 76,061,668 bp
  • A to T, chromosome 12 at 79,268,557 bp
  • T to C, chromosome 13 at 12,288,630 bp
  • T to C, chromosome 14 at 55,512,609 bp
  • T to A, chromosome 15 at 101,231,909 bp
  • T to C, chromosome 16 at 36,494,811 bp
  • G to A, chromosome 17 at 23,837,633 bp
  • G to T, chromosome 17 at 27,707,060 bp
  • A to T, chromosome 18 at 34,828,546 bp
  • T to A, chromosome 18 at 37,402,308 bp
  • C to A, chromosome 18 at 46,926,343 bp
  • T to C, chromosome 18 at 74,206,836 bp
  • T to C, chromosome 18 at 84,094,526 bp
  • C to G, chromosome 19 at 60,467,309 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8141 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067569-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.