Strain Name:
C57BL/6J-MtgxR8159Btlr/Mmmh
Stock Number:
067585-MU
Citation ID:
RRID:MMRRC_067585-MU
Other Names:
R8159 (G1)
Major Collection:

Strain Information

Notch2
Name: notch 2
Synonyms: Motch B, N2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18129
HGNC: HGNC:7882
Homologene: 7865
Ptprz1
Name: protein tyrosine phosphatase receptor type Z, polypeptide 1
Synonyms: Ptprz, Ptpz, Rptpbeta, RPTPz, DSD-1-PG, PTPbeta, phosphacan, PTPzeta
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19283
HGNC: HGNC:9685
Homologene: 2136
Hes5
Name: hes family bHLH transcription factor 5
Synonyms: bHLHb38
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 15208
Homologene: 7755
D630045J12Rik
Name: RIKEN cDNA D630045J12 gene
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330286
Homologene: 19782
Spen
Name: spen family transcription repressor
Synonyms: Mint
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56381
Homologene: 124461
Agrn
Name: agrin
Synonyms: NMF380, nmf380, Agrin
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11603
HGNC: HGNC:329
Homologene: 27907
Auts2
Name: autism susceptibility candidate 2
Synonyms: D830032G16Rik, 2700063G02Rik, A730011F23Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 319974
Homologene: 22907
Fry
Name: FRY microtubule binding protein
Synonyms: cg003, 9330186A19Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 320365
Homologene: 113770
Gigyf1
Name: GRB10 interacting GYF protein 1
Synonyms: Perq1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 57330
HGNC: HGNC:9126
Homologene: 57145
Smg6
Name: SMG6 nonsense mediated mRNA decay factor
Synonyms: Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans)
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103677
Homologene: 23024
Herc1
Name: HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Synonyms: D130015N03Rik, 2810449H11Rik, tbl
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235439
HGNC: HGNC:4867
Homologene: 31207
Aff4
Name: AF4/FMR2 family, member 4
Synonyms: Laf4l, Alf4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 93736
Homologene: 8683
Pals2
Name: protein associated with LIN7 2, MAGUK family member
Synonyms: Mpp6, P55t, Pals2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 56524
Homologene: 22976
Otof
Name: otoferlin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 83762
HGNC: HGNC:8515
Homologene: 12892
Cd72
Name: CD72 antigen
Synonyms: Ly-19, Ly-m19, Ly-32, Lyb-2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12517
HGNC: HGNC:1696
Homologene: 1350
Rfx2
Name: regulatory factor X, 2 (influences HLA class II expression)
Synonyms: 5430432H19Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 19725
VEGA: 17
HGNC: HGNC:9983
Homologene: 30980
Chpf
Name: chondroitin polymerizing factor
Synonyms: D1Bwg1363e, 1700028N03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74241
Homologene: 11574
Dkk2
Name: dickkopf WNT signaling pathway inhibitor 2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 56811
HGNC: HGNC:2892
Homologene: 8681
Chst9
Name: carbohydrate sulfotransferase 9
Synonyms: GalNAc4ST-2, 5430438D01Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 71367
Homologene: 12861
Gfra2
Name: glial cell line derived neurotrophic factor family receptor alpha 2
Synonyms: GFR alpha 2, GFR alpha-2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 14586
VEGA: 14
HGNC: HGNC:4244
Homologene: 1145
Svep1
Name: sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1
Synonyms: 1110021D17Rik, Polydom, D430029O09Rik, 4833413O10Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 64817
Homologene: 23386
Fhip2a
Name: FHF complex subunit HOOK interacting protein 2A
Synonyms: Fam160b1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 226252
VEGA: 19
Homologene: 28133
Insrr
Name: insulin receptor-related receptor
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 23920
HGNC: HGNC:6093
Homologene: 56539
Nynrin
Name: NYN domain and retroviral integrase containing
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 277154
VEGA: 14
Homologene: 19483
Irs1
Name: insulin receptor substrate 1
Synonyms: IRS-1, G972R
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16367
HGNC: HGNC:6125
Homologene: 4049
Sycp2
Name: synaptonemal complex protein 2
Synonyms: 3830402K23Rik, 4930518F03Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 320558
Homologene: 8604
Col14a1
Name: collagen, type XIV, alpha 1
Synonyms: 5730412L22Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12818
HGNC: HGNC:2191
Homologene: 18741
Ece2
Name: endothelin converting enzyme 2
Synonyms: 9630025D12Rik, 6330509A19Rik, 1810009K13Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 107522
Homologene: 56679
Slc9a3
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 3
Synonyms: NHE-3, NHE3, 9030624O13Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 105243
VEGA: 13
Homologene: 55804
BC005537
Name: cDNA sequence BC005537
Synonyms: 8030460C05Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 79555
Homologene: 11551
Aadacl2fm1
Name: AADACL2 family member 1
Synonyms: C130079G13Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229333
Vil1
Name: villin 1
Synonyms: Villin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 22349
Homologene: 5169
Ceacam20
Name: CEA cell adhesion molecule 20
Synonyms: 9130012D09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71601
Homologene: 19010
Cacna2d4
Name: calcium channel, voltage-dependent, alpha 2/delta subunit 4
Synonyms: 5730412N02Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 319734
Homologene: 26544
Phldb2
Name: pleckstrin homology like domain, family B, member 2
Synonyms: LL5b, C820004H04Rik, LL5beta
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 208177
Homologene: 17100
Tsen54
Name: tRNA splicing endonuclease subunit 54
Synonyms: 0610034P02Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76265
Homologene: 35476
Cntn5
Name: contactin 5
Synonyms: A830025P08Rik, LOC244683, 6720426O10Rik, NB-2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244682
HGNC: HGNC:2175
Homologene: 28447
Abcc1
Name: ATP-binding cassette, sub-family C member 1
Synonyms: Mdrap, MRP, Mrp1, Abcc1a, Abcc1b
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 17250
HGNC: HGNC:51
Homologene: 133779
Gpr180
Name: G protein-coupled receptor 180
Synonyms: ITR, E130016I23Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 58245
VEGA: 14
Homologene: 32506
Or7e178
Name: olfactory receptor family 7 subfamily E member 178
Synonyms: GA_x6K02T2PVTD-14054886-14053957, Olfr18, MOR145-1, MTPCR34
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18315
HGNC: HGNC:8396
Homologene: 138313
Or51v14
Name: olfactory receptor family 51 subfamily V member 14
Synonyms: Olfr620, GA_x6K02T2PBJ9-6335095-6334154, MOR4-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258808
Homologene: 103777
Uba1y
Name: ubiquitin-activating enzyme, Chr Y
Synonyms: Sby, A1s9Y-1, Ube1y1, Ube-2, Ube1y-1
Type: Gene
Species: Mouse
Chromosome: Y
NCBI: 22202
Homologene: 129435
Mettl22
Name: methyltransferase 22, Kin17 lysine
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239706
Homologene: 16619
Adrb3
Name: adrenergic receptor, beta 3
Synonyms: beta 3-AR, beta3-adrenergic receptor, Beta-3 adrenoceptor, Beta-3 AR, Adrb-3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11556
HGNC: HGNC:288
Homologene: 37250
Pcdhgc5
Name: protocadherin gamma subfamily C, 5
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93708
HGNC: HGNC:8718
Homologene: 110933
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 74,423,977 bp
  • C to T, chromosome 1 at 75,478,792 bp
  • A to C, chromosome 1 at 82,288,569 bp
  • A to C, chromosome 2 at 178,354,977 bp
  • C to A, chromosome 3 at 59,936,422 bp
  • T to A, chromosome 3 at 87,800,428 bp
  • A to G, chromosome 3 at 98,120,922 bp
  • T to C, chromosome 3 at 132,174,978 bp
  • A to G, chromosome 4 at 43,450,174 bp
  • C to T, chromosome 4 at 58,069,396 bp
  • T to C, chromosome 4 at 58,087,815 bp
  • A to T, chromosome 4 at 141,475,003 bp
  • A to G, chromosome 4 at 154,961,045 bp
  • G to A, chromosome 4 at 156,172,368 bp
  • C to T, chromosome 5 at 30,380,194 bp
  • A to G, chromosome 5 at 131,460,125 bp
  • A to T, chromosome 5 at 137,522,195 bp
  • A to T, chromosome 5 at 150,399,533 bp
  • A to G, chromosome 6 at 23,001,663 bp
  • T to C, chromosome 6 at 38,128,475 bp
  • A to T, chromosome 6 at 50,194,547 bp
  • A to G, chromosome 6 at 119,297,527 bp
  • G to T, chromosome 7 at 19,976,184 bp
  • G to A, chromosome 7 at 103,612,140 bp
  • A to C, chromosome 8 at 27,228,071 bp
  • A to G, chromosome 9 at 10,145,381 bp
  • A to C, chromosome 9 at 20,314,719 bp
  • A to G, chromosome 9 at 66,461,721 bp
  • A to T, chromosome 11 at 53,411,894 bp
  • G to A, chromosome 11 at 75,038,639 bp
  • T to A, chromosome 11 at 115,820,978 bp
  • T to A, chromosome 13 at 24,809,933 bp
  • A to G, chromosome 13 at 74,164,288 bp
  • T to G, chromosome 14 at 55,863,130 bp
  • A to T, chromosome 14 at 55,865,060 bp
  • A to G, chromosome 14 at 70,895,957 bp
  • G to A, chromosome 14 at 118,153,890 bp
  • A to G, chromosome 15 at 55,427,928 bp
  • T to C, chromosome 16 at 8,488,769 bp
  • C to T, chromosome 16 at 14,472,930 bp
  • C to T, chromosome 16 at 20,611,784 bp
  • T to C, chromosome 16 at 45,860,384 bp
  • A to T, chromosome 17 at 56,803,605 bp
  • A to T, chromosome 18 at 15,452,308 bp
  • A to G, chromosome 18 at 37,821,122 bp
  • T to C, chromosome 19 at 57,384,265 bp
  • T to A, chromosome Y at 828,806 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8159 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067585-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.