Strain Name:
C57BL/6J-MtgxR8162Btlr/Mmmh
Stock Number:
067588-MU
Citation ID:
RRID:MMRRC_067588-MU
Other Names:
R8162 (G1)
Major Collection:

Strain Information

Met
Name: met proto-oncogene
Synonyms: Par4, c-Met, HGF receptor
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17295
HGNC: HGNC:7029
Homologene: 206
Vcan
Name: versican
Synonyms: Cspg2, hdf, PG-M, heart defect, DPEAAE, 5430420N07Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
Phf12
Name: PHD finger protein 12
Synonyms: PF1, 2410142K10Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 268448
Homologene: 10841
Kdm2b
Name: lysine (K)-specific demethylase 2B
Synonyms: Fbxl10, Jhdm1b, Cxxc2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 30841
Homologene: 13069
Tango6
Name: transport and golgi organization 6
Synonyms: Tmco7, Tango6
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 272538
Homologene: 52121
Rapgef1
Name: Rap guanine nucleotide exchange factor (GEF) 1
Synonyms: 4932418O06Rik, Grf2, C3G
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 107746
HGNC: HGNC:4568
Homologene: 50501
Ptcd3
Name: pentatricopeptide repeat domain 3
Synonyms: 2610034F17Rik, 2810422B04Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 69956
Homologene: 41211
Ash1l
Name: ASH1 like histone lysine methyltransferase
Synonyms: chromatin remodeling factor, E430018P19Rik, KMT2H, 8030453L17Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 192195
Homologene: 10225
Tasor2
Name: transcription activation suppressor family member 2
Synonyms: BC016423, Fam208b
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 105203
VEGA: 13
Homologene: 26435
Tent2
Name: terminal nucleotidyltransferase 2
Synonyms: 8030446C20Rik, Papd4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 100715
VEGA: 13
Homologene: 44022
Dand5
Name: DAN domain family member 5, BMP antagonist
Synonyms: coco, Cerl-2, Cerl2, GREM3, Cerr2, Dte
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 23863
VEGA: 8
Homologene: 51883
Clec4a2
Name: C-type lectin domain family 4, member a2
Synonyms: Clecsf6, Dcir1, DCIR, dendritic cell immunoreceptor
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 26888
Homologene: 49291
Rnf26rt
Name: ring finger protein 26, retrotransposed
Synonyms: Gm9008
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 668155
Prf1
Name: perforin 1 (pore forming protein)
Synonyms: Pfp, Prf-1, Pfn, perforin
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18646
VEGA: 10
HGNC: HGNC:9360
Homologene: 3698
Idh2
Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial
Synonyms: IDPm, Idh-2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 269951
HGNC: HGNC:5383
Homologene: 37590
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Il1a
Name: interleukin 1 alpha
Synonyms: Il-1a
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16175
HGNC: HGNC:5991
Homologene: 480
Mcc
Name: mutated in colorectal cancers
Synonyms: D18Ertd451e
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 328949
HGNC: HGNC:6935
Homologene: 20539
Cpne8
Name: copine VIII
Synonyms: 1200003E11Rik, 1500031E20Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 66871
Homologene: 12049
Vwf
Name: Von Willebrand factor
Synonyms: 6820430P06Rik, B130011O06Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 22371
Homologene: 466
Farp2
Name: FERM, RhoGEF and pleckstrin domain protein 2
Synonyms: Fir, D030026M03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227377
Homologene: 8877
Tbc1d2b
Name: TBC1 domain family, member 2B
Synonyms: 1810061M12Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 67016
Homologene: 56694
Cacna1e
Name: calcium channel, voltage-dependent, R type, alpha 1E subunit
Synonyms: alpha1E, Cchra1, Cav2.3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12290
HGNC: HGNC:1392
Homologene: 20185
Cpeb2
Name: cytoplasmic polyadenylation element binding protein 2
Synonyms: A630055H10Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231207
Homologene: 17995
Tsks
Name: testis-specific serine kinase substrate
Synonyms: Tsks, Stk22s1, Tssks1, clone 4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22116
Homologene: 7984
Ccdc40
Name: coiled-coil domain containing 40
Synonyms: B930008I02Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 207607
Homologene: 27890
Or5p63
Name: olfactory receptor family 5 subfamily P member 63
Synonyms: MOR204-31P, GA_x6K02T2PBJ9-10541702-10540758, Olfr487, MOR204-29P, Olfr1538-ps1, MOR204-31P
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258042
Homologene: 110483
Gtf3c4
Name: general transcription factor IIIC, polypeptide 4
Synonyms: KAT12
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 269252
HGNC: HGNC:4667
Homologene: 8147
Ces3b
Name: carboxylesterase 3B
Synonyms: Gm4738, ES31L
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 13909
HGNC: HGNC:1865
Homologene: 84407
Or9s18
Name: olfactory receptor family 9 subfamily S member 18
Synonyms: Olfr466, GA_x6K02T2PB7A-3051266-3052192, MOR209-1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 258816
Homologene: 72032
Evi5l
Name: ecotropic viral integration site 5 like
Synonyms: 1700084G18Rik, B130050I23Rik, 2310039H16Rik, 3110007G05Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 213027
Homologene: 134635
Ocstamp
Name: osteoclast stimulatory transmembrane protein
Synonyms: 4833422F24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74614
Homologene: 41768
Mrgpra4
Name: MAS-related GPR, member A4
Synonyms: MrgA4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 235854
Homologene: 131159
Zfp827
Name: zinc finger protein 827
Synonyms: 2810449M09Rik, D630040G17Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 622675
Homologene: 45622
Apol7b
Name: apolipoprotein L 7b
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 278679
HGNC: HGNC:619
Homologene: 40940
Or8g17
Name: olfactory receptor family 8 subfamily G member 17
Synonyms: Olfr146, GA_x6K02T2PVTD-32715386-32714466, MOR171-10, M15
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258742
VEGA: 9
Rftn1
Name: raftlin lipid raft linker 1
Synonyms: 2310015N21Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 76438
Homologene: 18745
Erich3
Name: glutamate rich 3
Synonyms: 5031409G23Rik, 4922501L14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 209601
Homologene: 27877
Nutm1
Name: NUT midline carcinoma, family member 1
Synonyms: Nut, BC125332
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 213765
Homologene: 18185
Asb14
Name: ankyrin repeat and SOCS box-containing 14
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 142687
Homologene: 15445
Mogs
Name: mannosyl-oligosaccharide glucosidase
Synonyms: Gcs1, 1810017N02Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 57377
Homologene: 4593
Or1ab2
Name: olfactory receptor family 1 subfamily AB member 2
Synonyms: MOR130-1, GA_x6K02T2NUPS-241490-242431, Olfr374
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 258335
Homologene: 131127
Cd27
Name: CD27 antigen
Synonyms: Cd27, Tp55, S152, Tnfrsf7
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 21940
Homologene: 74386
D630039A03Rik
Name: RIKEN cDNA D630039A03 gene
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242484
Homologene: 52276
Gm8674
Name: predicted gene 8674
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 667507
VEGA: 13
Homologene: 140547
Trav10n
Name: T cell receptor alpha variable 10N
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 667626
Gm13741
Name: predicted gene 13741
Type: Gene
Species: Mouse
Chromosome: 2
Ms4a18
Name: membrane-spanning 4-domains, subfamily A, member 18
Synonyms: 5033428B15Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 76002
Homologene: 89543
Gm28363
Name: predicted gene 28363
Type: Gene
Species: Mouse
Chromosome: 1
G530012D18Rik
Name: RIKEN cDNA G530012D1 gene
Type: Gene
Species: Mouse
Chromosome: 1
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to G, chromosome 1 at 85,577,214 bp
  • C to A, chromosome 1 at 93,620,603 bp
  • T to A, chromosome 1 at 117,698,911 bp
  • G to T, chromosome 1 at 154,701,567 bp
  • G to A, chromosome 2 at 28,834,581 bp
  • A to T, chromosome 2 at 29,735,999 bp
  • A to C, chromosome 2 at 87,656,102 bp
  • G to A, chromosome 2 at 112,248,472 bp
  • A to T, chromosome 2 at 129,306,557 bp
  • G to T, chromosome 2 at 165,397,867 bp
  • T to G, chromosome 3 at 89,070,246 bp
  • C to T, chromosome 3 at 154,764,573 bp
  • T to C, chromosome 4 at 57,910,525 bp
  • A to T, chromosome 5 at 43,237,338 bp
  • A to T, chromosome 5 at 122,934,793 bp
  • A to G, chromosome 6 at 17,547,062 bp
  • A to G, chromosome 6 at 71,907,814 bp
  • C to A, chromosome 6 at 76,496,575 bp
  • T to C, chromosome 6 at 83,115,882 bp
  • T to A, chromosome 6 at 123,140,752 bp
  • T to A, chromosome 6 at 125,233,225 bp
  • T to A, chromosome 6 at 125,645,836 bp
  • A to G, chromosome 7 at 44,953,872 bp
  • A to T, chromosome 7 at 47,981,473 bp
  • GGTCCCAG to GG, chromosome 7 at 80,098,329 bp
  • T to C, chromosome 7 at 108,211,788 bp
  • T to C, chromosome 8 at 4,191,300 bp
  • T to G, chromosome 8 at 72,110,409 bp
  • T to A, chromosome 8 at 79,060,577 bp
  • C to A, chromosome 8 at 84,816,518 bp
  • T to C, chromosome 8 at 105,090,753 bp
  • C to T, chromosome 8 at 106,683,250 bp
  • T to A, chromosome 9 at 39,018,953 bp
  • C to T, chromosome 9 at 90,207,884 bp
  • A to G, chromosome 10 at 61,302,970 bp
  • C to A, chromosome 11 at 78,024,825 bp
  • G to T, chromosome 11 at 119,260,044 bp
  • G to T, chromosome 13 at 3,599,691 bp
  • A to T, chromosome 13 at 49,900,371 bp
  • G to A, chromosome 13 at 65,152,920 bp
  • G to T, chromosome 13 at 89,704,987 bp
  • A to G, chromosome 13 at 93,167,924 bp
  • A to T, chromosome 14 at 26,911,988 bp
  • A to T, chromosome 14 at 53,122,472 bp
  • T to A, chromosome 15 at 77,423,230 bp
  • T to C, chromosome 15 at 90,619,678 bp
  • A to T, chromosome 16 at 32,750,379 bp
  • G to T, chromosome 17 at 50,047,380 bp
  • T to C, chromosome 18 at 44,449,441 bp
  • A to G, chromosome 19 at 11,013,707 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8162 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067588-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.