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Strain Name:
C57BL/6J-MtgxR8170Btlr/Mmmh
Stock Number:
067596-MU
Citation ID:
RRID:MMRRC_067596-MU
Other Names:
R8170 (G1)
Major Collection:

Strain Information

Ednrb
Name: endothelin receptor type B
Synonyms: ETb, Sox10m1, ETR-b
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13618
VEGA: 14
HGNC: HGNC:3180
Homologene: 89
Ppp5c
Name: protein phosphatase 5, catalytic subunit
Synonyms: ANP receptor, PP5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 19060
HGNC: HGNC:9322
Homologene: 4550
Atg7
Name: autophagy related 7
Synonyms: 1810013K23Rik, Apg7l
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74244
Homologene: 4662
Acadm
Name: acyl-Coenzyme A dehydrogenase, medium chain
Synonyms: MCAD
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11364
HGNC: HGNC:89
Homologene: 3
Mta3
Name: metastasis associated 3
Synonyms: 1110002J22Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 116871
Homologene: 14282
Ylpm1
Name: YLP motif containing 1
Synonyms: ZAP, Zap3, A930013E17Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 56531
Homologene: 87707
Ctdspl2
Name: CTD small phosphatase like 2
Synonyms: D2Ertd485e, SCP4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 329506
Homologene: 32311
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to C, chromosome 1 at 60,237,151 bp
  • G to T, chromosome 1 at 66,651,533 bp
  • T to C, chromosome 1 at 84,285,000 bp
  • T to C, chromosome 1 at 92,934,964 bp
  • T to A, chromosome 1 at 136,940,647 bp
  • A to G, chromosome 2 at 24,678,874 bp
  • G to C, chromosome 2 at 33,015,223 bp
  • C to A, chromosome 2 at 118,711,453 bp
  • T to C, chromosome 2 at 120,700,048 bp
  • T to G, chromosome 2 at 122,006,942 bp
  • A to T, chromosome 3 at 109,424,007 bp
  • T to A, chromosome 3 at 153,944,398 bp
  • C to T, chromosome 4 at 32,615,490 bp
  • T to A, chromosome 4 at 33,162,718 bp
  • T to A, chromosome 4 at 58,069,378 bp
  • T to A, chromosome 4 at 59,211,974 bp
  • T to A, chromosome 4 at 95,050,322 bp
  • C to T, chromosome 4 at 149,718,720 bp
  • T to C, chromosome 4 at 152,280,177 bp
  • T to A, chromosome 4 at 152,376,583 bp
  • A to C, chromosome 5 at 17,374,461 bp
  • A to G, chromosome 5 at 31,245,803 bp
  • A to G, chromosome 5 at 138,647,053 bp
  • T to A, chromosome 5 at 140,426,775 bp
  • A to T, chromosome 5 at 144,277,692 bp
  • A to G, chromosome 6 at 25,758,803 bp
  • A to T, chromosome 6 at 30,624,595 bp
  • G to A, chromosome 6 at 42,912,191 bp
  • T to C, chromosome 6 at 71,129,333 bp
  • C to G, chromosome 6 at 71,920,749 bp
  • A to T, chromosome 6 at 114,701,190 bp
  • A to T, chromosome 6 at 120,206,616 bp
  • T to A, chromosome 6 at 123,853,017 bp
  • G to T, chromosome 7 at 17,007,146 bp
  • C to A, chromosome 7 at 19,057,580 bp
  • A to T, chromosome 7 at 23,433,710 bp
  • A to C, chromosome 7 at 79,433,557 bp
  • A to G, chromosome 7 at 92,561,542 bp
  • C to T, chromosome 7 at 120,465,782 bp
  • G to A, chromosome 7 at 141,861,000 bp
  • A to T, chromosome 8 at 95,320,058 bp
  • T to A, chromosome 8 at 110,866,031 bp
  • T to G, chromosome 8 at 111,080,919 bp
  • C to T, chromosome 8 at 119,602,826 bp
  • T to C, chromosome 9 at 15,947,496 bp
  • T to A, chromosome 9 at 45,043,721 bp
  • T to G, chromosome 9 at 63,429,030 bp
  • T to A, chromosome 10 at 79,826,466 bp
  • G to A, chromosome 10 at 80,027,872 bp
  • T to A, chromosome 10 at 80,803,297 bp
  • A to C, chromosome 10 at 86,776,166 bp
  • A to G, chromosome 10 at 127,187,179 bp
  • T to A, chromosome 10 at 129,269,048 bp
  • T to C, chromosome 11 at 53,537,857 bp
  • C to T, chromosome 11 at 55,270,455 bp
  • A to G, chromosome 11 at 67,288,266 bp
  • T to A, chromosome 12 at 85,034,027 bp
  • T to G, chromosome 12 at 112,175,318 bp
  • G to A, chromosome 13 at 3,574,881 bp
  • T to C, chromosome 13 at 15,720,208 bp
  • T to C, chromosome 13 at 27,352,811 bp
  • C to T, chromosome 13 at 40,719,268 bp
  • A to T, chromosome 13 at 64,215,735 bp
  • T to C, chromosome 13 at 77,263,594 bp
  • A to T, chromosome 14 at 77,091,878 bp
  • T to A, chromosome 14 at 103,823,204 bp
  • C to A, chromosome 15 at 76,363,619 bp
  • A to G, chromosome 15 at 76,911,371 bp
  • T to C, chromosome 15 at 89,548,840 bp
  • A to G, chromosome 15 at 103,009,861 bp
  • G to T, chromosome 16 at 5,011,737 bp
  • T to A, chromosome 17 at 30,673,823 bp
  • T to C, chromosome 17 at 79,856,261 bp
  • T to C, chromosome 17 at 80,605,860 bp
  • T to C, chromosome 17 at 83,791,661 bp
  • A to G, chromosome 18 at 37,475,584 bp
  • G to T, chromosome 19 at 8,771,050 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8170 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067596-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.