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Strain Name:
C57BL/6J-MtgxR8181Btlr/Mmmh
Stock Number:
067605-MU
Citation ID:
RRID:MMRRC_067605-MU
Other Names:
R8181 (G1)
Major Collection:

Strain Information

Sptbn4
Name: spectrin beta, non-erythrocytic 4
Synonyms: dyn, SpbIV, neuroaxonal dystrophy, 5830426A08Rik, ROSA62, nmf261, 1700022P15Rik, Spnb4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 80297
Homologene: 11879
Calca
Name: calcitonin/calcitonin-related polypeptide, alpha
Synonyms: Cgrp, CA, alpha CGRP, Ctn, Ct, Calc, CT
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12310
HGNC: HGNC:1437
Homologene: 88337
Lars1
Name: leucyl-tRNA synthetase 1
Synonyms: 3110009L02Rik, 2310045K21Rik, Lars
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 107045
VEGA: 18
HGNC: HGNC:6512
Homologene: 7083
Dscaml1
Name: DS cell adhesion molecule like 1
Synonyms: 4930435C18Rik, 4921507G06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 114873
VEGA: 9
Homologene: 79549
Ticrr
Name: TOPBP1-interacting checkpoint and replication regulator
Synonyms: 5730590G19Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 77011
Homologene: 67120
Prpf38a
Name: PRP38 pre-mRNA processing factor 38 (yeast) domain containing A
Synonyms: 2410002M20Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230596
Homologene: 13146
Kif11
Name: kinesin family member 11
Synonyms: Eg5, Knsl1, Kifl1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16551
VEGA: 19
HGNC: HGNC:6388
Homologene: 3322
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 55,363,319 bp
  • G to T, chromosome 1 at 133,383,760 bp
  • A to T, chromosome 2 at 10,575,379 bp
  • A to C, chromosome 2 at 52,474,985 bp
  • G to C, chromosome 2 at 76,639,290 bp
  • T to A, chromosome 2 at 111,257,573 bp
  • T to G, chromosome 2 at 112,778,243 bp
  • T to C, chromosome 2 at 129,209,127 bp
  • T to A, chromosome 2 at 137,000,184 bp
  • A to T, chromosome 2 at 164,950,445 bp
  • A to C, chromosome 3 at 59,261,968 bp
  • T to C, chromosome 3 at 98,162,975 bp
  • A to T, chromosome 3 at 98,856,137 bp
  • A to C, chromosome 3 at 106,149,887 bp
  • A to G, chromosome 3 at 108,689,764 bp
  • T to A, chromosome 3 at 108,752,012 bp
  • A to G, chromosome 3 at 117,322,465 bp
  • G to A, chromosome 3 at 138,068,395 bp
  • T to C, chromosome 3 at 144,839,137 bp
  • A to C, chromosome 4 at 46,396,504 bp
  • A to G, chromosome 4 at 53,059,303 bp
  • G to A, chromosome 4 at 108,576,998 bp
  • G to T, chromosome 5 at 64,112,124 bp
  • T to A, chromosome 5 at 104,211,514 bp
  • G to A, chromosome 5 at 137,210,078 bp
  • A to T, chromosome 5 at 144,191,426 bp
  • A to G, chromosome 5 at 150,063,736 bp
  • T to C, chromosome 6 at 3,693,899 bp
  • T to C, chromosome 7 at 14,470,173 bp
  • C to A, chromosome 7 at 19,724,808 bp
  • T to C, chromosome 7 at 27,375,383 bp
  • T to C, chromosome 7 at 28,331,474 bp
  • A to G, chromosome 7 at 29,793,655 bp
  • G to T, chromosome 7 at 45,466,274 bp
  • T to C, chromosome 7 at 79,660,980 bp
  • A to T, chromosome 7 at 85,956,116 bp
  • A to G, chromosome 7 at 114,635,152 bp
  • A to G, chromosome 7 at 132,983,779 bp
  • A to G, chromosome 8 at 22,243,006 bp
  • T to C, chromosome 8 at 45,381,718 bp
  • A to G, chromosome 8 at 110,852,085 bp
  • A to T, chromosome 8 at 123,361,303 bp
  • A to T, chromosome 9 at 15,277,632 bp
  • C to T, chromosome 9 at 45,049,708 bp
  • T to A, chromosome 9 at 45,746,842 bp
  • C to T, chromosome 9 at 114,430,361 bp
  • A to G, chromosome 10 at 7,120,475 bp
  • T to A, chromosome 10 at 43,986,326 bp
  • T to A, chromosome 11 at 49,634,896 bp
  • C to A, chromosome 11 at 52,213,269 bp
  • A to T, chromosome 11 at 100,376,925 bp
  • T to A, chromosome 11 at 105,129,040 bp
  • A to G, chromosome 11 at 107,672,573 bp
  • T to A, chromosome 11 at 119,321,892 bp
  • A to G, chromosome 12 at 72,940,132 bp
  • A to G, chromosome 12 at 100,850,111 bp
  • G to A, chromosome 13 at 4,135,263 bp
  • A to G, chromosome 13 at 9,689,651 bp
  • G to T, chromosome 13 at 47,051,901 bp
  • T to A, chromosome 14 at 29,021,937 bp
  • T to A, chromosome 14 at 32,557,948 bp
  • C to A, chromosome 14 at 55,672,984 bp
  • A to G, chromosome 15 at 79,725,520 bp
  • T to A, chromosome 16 at 36,916,830 bp
  • A to T, chromosome 16 at 97,892,302 bp
  • G to T, chromosome 17 at 39,539,816 bp
  • T to A, chromosome 17 at 56,429,064 bp
  • A to T, chromosome 18 at 42,228,770 bp
  • T to C, chromosome 19 at 10,981,306 bp
  • T to A, chromosome 19 at 11,603,289 bp
  • A to T, chromosome 19 at 37,390,647 bp
  • G to T, chromosome 19 at 40,557,437 bp
  • G to T, chromosome 19 at 40,913,971 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8181 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067605-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.