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Strain Name:
C57BL/6J-MtgxR8186Btlr/Mmmh
Stock Number:
067609-MU
Citation ID:
RRID:MMRRC_067609-MU
Other Names:
R8186 (G1)
Major Collection:

Strain Information

Utrn
Name: utrophin
Synonyms: G-utrophin, Dmdl, DRP
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398
Htr5b
Name: 5-hydroxytryptamine (serotonin) receptor 5B
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 15564
Homologene: 74951
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Lrrc2
Name: leucine rich repeat containing 2
Synonyms: 2400002D05Rik, 4933431K03Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74249
Homologene: 23454
Usp48
Name: ubiquitin specific peptidase 48
Synonyms: D330022K21Rik, 2810449C13Rik, Usp31
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 170707
Homologene: 12988
Chd9
Name: chromodomain helicase DNA binding protein 9
Synonyms: 1810014J18Rik, AD013, A330063D19Rik, 9030205D12Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 109151
Homologene: 11844
Ddx19a
Name: DEAD box helicase 19a
Synonyms: Eif4a-rs1, DBP5, Ddx19, DEAD (Asp-Glu-Ala-Asp) box polypeptide 19a
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 13680
Homologene: 5240
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 5,602,317 bp
  • G to C, chromosome 1 at 60,237,151 bp
  • T to C, chromosome 1 at 78,416,972 bp
  • G to A, chromosome 1 at 121,528,379 bp
  • A to G, chromosome 1 at 166,100,079 bp
  • T to G, chromosome 2 at 76,918,912 bp
  • C to T, chromosome 2 at 131,189,130 bp
  • A to G, chromosome 3 at 116,758,908 bp
  • T to A, chromosome 4 at 52,610,489 bp
  • G to A, chromosome 4 at 101,890,834 bp
  • A to T, chromosome 4 at 123,372,426 bp
  • A to C, chromosome 4 at 123,382,130 bp
  • A to G, chromosome 4 at 136,051,657 bp
  • A to G, chromosome 4 at 137,621,196 bp
  • A to G, chromosome 5 at 11,773,959 bp
  • A to G, chromosome 5 at 72,559,012 bp
  • T to C, chromosome 6 at 22,123,588 bp
  • G to A, chromosome 6 at 86,108,020 bp
  • A to G, chromosome 7 at 6,373,229 bp
  • C to T, chromosome 7 at 101,296,507 bp
  • T to C, chromosome 8 at 11,425,542 bp
  • T to A, chromosome 8 at 90,998,605 bp
  • T to C, chromosome 8 at 110,609,645 bp
  • T to A, chromosome 8 at 110,983,642 bp
  • C to A, chromosome 8 at 123,461,476 bp
  • A to G, chromosome 9 at 110,960,842 bp
  • T to C, chromosome 10 at 12,698,123 bp
  • G to A, chromosome 10 at 80,027,872 bp
  • T to A, chromosome 10 at 121,745,342 bp
  • C to A, chromosome 10 at 127,887,415 bp
  • A to T, chromosome 11 at 53,470,309 bp
  • T to C, chromosome 11 at 73,354,421 bp
  • A to G, chromosome 11 at 78,286,631 bp
  • A to G, chromosome 11 at 100,563,679 bp
  • T to C, chromosome 11 at 103,730,775 bp
  • A to G, chromosome 12 at 116,000,303 bp
  • A to G, chromosome 13 at 48,807,751 bp
  • A to T, chromosome 14 at 51,418,700 bp
  • A to G, chromosome 14 at 63,201,513 bp
  • A to C, chromosome 15 at 81,009,442 bp
  • C to A, chromosome 15 at 99,037,515 bp
  • A to G, chromosome 16 at 22,818,439 bp
  • A to T, chromosome 17 at 19,811,943 bp
  • G to T, chromosome 17 at 20,224,618 bp
  • A to T, chromosome 17 at 34,957,581 bp
  • G to A, chromosome 17 at 56,566,051 bp
  • T to A, chromosome 19 at 6,306,865 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8186 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067609-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.