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Strain Name:
C57BL/6J-MtgxR8191Btlr/Mmmh
Stock Number:
067614-MU
Citation ID:
RRID:MMRRC_067614-MU
Other Names:
R8191 (G1)
Major Collection:

Strain Information

Zfp143
Name: zinc finger protein 143
Synonyms: D7Ertd805e, pHZ-1, KRAB14, Zfp79, Zfp80-rs1, Staf
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20841
Homologene: 56444
Ubr5
Name: ubiquitin protein ligase E3 component n-recognin 5
Synonyms: 4432411E13Rik, Edd1, Edd
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 70790
VEGA: 15
Homologene: 9295
Xpa
Name: xeroderma pigmentosum, complementation group A
Synonyms: Xpac
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22590
Homologene: 37298
Srrm2
Name: serine/arginine repetitive matrix 2
Synonyms: 5033413A03Rik, SRm300
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 75956
Tlr2
Name: toll-like receptor 2
Synonyms: Ly105
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 24088
Homologene: 20695
Ccnk
Name: cyclin K
Synonyms: CPR4, CycK
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 12454
VEGA: 12
HGNC: HGNC:1596
Homologene: 14748
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 38,036,202 bp
  • A to G, chromosome 1 at 46,607,458 bp
  • T to C, chromosome 1 at 135,272,434 bp
  • C to T, chromosome 1 at 136,108,155 bp
  • C to T, chromosome 1 at 160,125,518 bp
  • T to C, chromosome 1 at 171,252,723 bp
  • T to C, chromosome 1 at 173,751,660 bp
  • T to A, chromosome 1 at 184,037,552 bp
  • T to C, chromosome 2 at 76,870,739 bp
  • T to C, chromosome 2 at 131,085,679 bp
  • C to T, chromosome 2 at 151,557,436 bp
  • C to A, chromosome 2 at 164,820,208 bp
  • G to T, chromosome 3 at 10,088,854 bp
  • T to A, chromosome 3 at 83,836,514 bp
  • T to G, chromosome 3 at 83,836,515 bp
  • A to G, chromosome 3 at 135,251,614 bp
  • G to T, chromosome 3 at 146,094,311 bp
  • C to A, chromosome 4 at 46,183,225 bp
  • A to T, chromosome 4 at 58,872,587 bp
  • G to A, chromosome 4 at 136,658,945 bp
  • T to C, chromosome 4 at 141,620,584 bp
  • T to G, chromosome 4 at 156,351,414 bp
  • T to A, chromosome 5 at 34,440,573 bp
  • T to A, chromosome 5 at 76,559,827 bp
  • T to A, chromosome 5 at 87,001,443 bp
  • T to A, chromosome 5 at 121,874,154 bp
  • T to G, chromosome 5 at 139,181,495 bp
  • T to C, chromosome 6 at 30,813,730 bp
  • C to A, chromosome 6 at 32,516,950 bp
  • T to C, chromosome 6 at 42,675,256 bp
  • C to T, chromosome 6 at 88,222,958 bp
  • G to A, chromosome 7 at 23,866,311 bp
  • G to T, chromosome 7 at 27,031,104 bp
  • T to A, chromosome 7 at 27,663,246 bp
  • A to G, chromosome 7 at 27,879,443 bp
  • T to C, chromosome 7 at 50,448,874 bp
  • C to T, chromosome 7 at 110,077,157 bp
  • A to G, chromosome 7 at 141,867,684 bp
  • T to C, chromosome 8 at 3,542,382 bp
  • C to A, chromosome 8 at 24,533,680 bp
  • C to A, chromosome 8 at 40,308,320 bp
  • C to A, chromosome 8 at 71,693,478 bp
  • A to T, chromosome 9 at 40,101,471 bp
  • C to A, chromosome 9 at 114,709,185 bp
  • T to A, chromosome 10 at 41,740,959 bp
  • T to G, chromosome 10 at 41,963,751 bp
  • A to G, chromosome 10 at 93,865,405 bp
  • T to A, chromosome 10 at 127,508,199 bp
  • T to C, chromosome 11 at 75,522,239 bp
  • T to C, chromosome 11 at 116,681,922 bp
  • A to T, chromosome 12 at 108,193,674 bp
  • A to T, chromosome 13 at 67,621,719 bp
  • A to T, chromosome 13 at 116,305,418 bp
  • A to G, chromosome 14 at 52,390,530 bp
  • C to A, chromosome 15 at 38,006,507 bp
  • G to A, chromosome 15 at 82,853,405 bp
  • C to T, chromosome 15 at 89,120,640 bp
  • A to T, chromosome 15 at 99,243,325 bp
  • G to A, chromosome 16 at 3,907,054 bp
  • A to T, chromosome 16 at 30,349,780 bp
  • C to A, chromosome 16 at 72,933,254 bp
  • G to T, chromosome 16 at 90,931,001 bp
  • A to G, chromosome 16 at 97,763,526 bp
  • A to T, chromosome 17 at 20,030,203 bp
  • A to G, chromosome 17 at 23,359,556 bp
  • T to A, chromosome 17 at 23,820,245 bp
  • C to G, chromosome 17 at 25,728,637 bp
  • A to T, chromosome 17 at 34,590,477 bp
  • A to G, chromosome 17 at 35,019,736 bp
  • T to A, chromosome 17 at 56,258,356 bp
  • C to T, chromosome 17 at 78,546,399 bp
  • A to G, chromosome 18 at 15,398,165 bp
  • G to T, chromosome 18 at 61,973,358 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8191 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067614-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.