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Strain Name:
C57BL/6J-MtgxR8229Btlr/Mmmh
Stock Number:
067645-MU
Citation ID:
RRID:MMRRC_067645-MU
Other Names:
R8229 (G1)
Major Collection:

Strain Information

Nedd4
Name: neural precursor cell expressed, developmentally down-regulated 4
Synonyms: Nedd4-1, Nedd4, Nedd4a, E430025J12Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17999
HGNC: HGNC:7727
Homologene: 134533
Limch1
Name: LIM and calponin homology domains 1
Synonyms: 3732412D22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77569
Homologene: 18953
Gli1
Name: GLI-Kruppel family member GLI1
Synonyms: Zfp-5, Zfp5
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14632
VEGA: 10
HGNC: HGNC:4317
Homologene: 3859
Sipa1l1
Name: signal-induced proliferation-associated 1 like 1
Synonyms: 4931426N11Rik, Spar
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217692
VEGA: 12
Homologene: 9189
Prepl
Name: prolyl endopeptidase-like
Synonyms: 9530014L06Rik, 2810457N15Rik, D030028O16Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 213760
VEGA: 17
Homologene: 15481
Zfp609
Name: zinc finger protein 609
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 214812
Homologene: 72220
Klhl24
Name: kelch-like 24
Synonyms: 6530402O20Rik, 1110046J11Rik, 4930429H24Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 75785
VEGA: 16
Homologene: 32352
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 2 at 82,978,143 bp
  • A to T, chromosome 2 at 87,753,064 bp
  • T to A, chromosome 2 at 89,075,038 bp
  • G to A, chromosome 2 at 132,869,887 bp
  • T to G, chromosome 2 at 164,014,095 bp
  • C to A, chromosome 3 at 104,015,701 bp
  • T to C, chromosome 3 at 104,015,702 bp
  • T to A, chromosome 3 at 146,901,449 bp
  • T to C, chromosome 3 at 152,370,292 bp
  • A to G, chromosome 5 at 67,028,795 bp
  • A to G, chromosome 5 at 86,809,308 bp
  • T to A, chromosome 5 at 105,606,536 bp
  • T to C, chromosome 6 at 119,753,288 bp
  • G to T, chromosome 6 at 126,967,003 bp
  • A to G, chromosome 7 at 46,997,774 bp
  • C to T, chromosome 7 at 108,066,587 bp
  • A to T, chromosome 8 at 24,711,738 bp
  • A to G, chromosome 8 at 79,673,975 bp
  • T to C, chromosome 9 at 65,703,500 bp
  • G to T, chromosome 9 at 72,731,388 bp
  • C to T, chromosome 10 at 127,332,448 bp
  • G to A, chromosome 11 at 58,981,341 bp
  • G to A, chromosome 11 at 97,453,906 bp
  • A to T, chromosome 11 at 116,947,387 bp
  • T to A, chromosome 12 at 11,291,672 bp
  • T to C, chromosome 12 at 82,437,848 bp
  • A to G, chromosome 12 at 112,611,596 bp
  • A to T, chromosome 14 at 59,331,281 bp
  • A to G, chromosome 15 at 9,367,377 bp
  • T to C, chromosome 16 at 20,114,571 bp
  • T to A, chromosome 17 at 15,059,334 bp
  • A to G, chromosome 17 at 28,217,755 bp
  • A to G, chromosome 17 at 36,056,376 bp
  • T to C, chromosome 17 at 36,324,039 bp
  • T to C, chromosome 17 at 85,081,261 bp
  • C to T, chromosome 18 at 12,407,551 bp
  • A to C, chromosome 18 at 23,971,680 bp
  • A to G, chromosome 18 at 32,084,687 bp
  • C to A, chromosome 18 at 36,974,723 bp
  • A to G, chromosome 18 at 37,422,618 bp
  • A to G, chromosome 19 at 13,028,197 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8229 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067645-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.