Strain Name:
C57BL/6J-MtgxR8243Btlr/Mmmh
Stock Number:
067648-MU
Citation ID:
RRID:MMRRC_067648-MU
Other Names:
R8243 (G1)
Major Collection:

Strain Information

Hps5
Name: HPS5, biogenesis of lysosomal organelles complex 2 subunit 2
Synonyms: ru2, Hermansky-Pudlak syndrome 5, ru-2, ruby eye 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 246694
Homologene: 35333
Efemp1
Name: epidermal growth factor-containing fibulin-like extracellular matrix protein 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216616
HGNC: HGNC:3218
Homologene: 3028
Kif1b
Name: kinesin family member 1B
Synonyms: Kif1b beta, A530096N05Rik, N-3 kinesin, D4Mil1e, KIF1Bp204, KIF1Bp130, Kif1b alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 16561
Homologene: 99835
Mark3
Name: MAP/microtubule affinity regulating kinase 3
Synonyms: A430080F22Rik, ETK-1, 1600015G02Rik, C-TAK1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 17169
VEGA: 12
HGNC: HGNC:6897
Homologene: 55653
Rffl
Name: ring finger and FYVE like domain containing protein
Synonyms: Carp2, fring, Carp-2, 4930516L10Rik, 1700051E09Rik, rififylin
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67338
Homologene: 12116
Bcl6
Name: B cell leukemia/lymphoma 6
Synonyms: Bcl5
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12053
HGNC: HGNC:1001
Homologene: 7640
Utp25
Name: UTP25 small subunit processome component
Synonyms: AA408296, Diexf, mDef
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 215193
Homologene: 6170
Dnajc8
Name: DnaJ heat shock protein family (Hsp40) member C8
Synonyms: 2010009J04Rik, 1110021D09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68598
Homologene: 40935
Helq
Name: helicase, POLQ-like
Synonyms: D430018E21Rik, Hel308
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 191578
Homologene: 14667
Gna13
Name: guanine nucleotide binding protein, alpha 13
Synonyms: Galpha13
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14674
HGNC: HGNC:4381
Homologene: 55976
P4hb
Name: prolyl 4-hydroxylase, beta polypeptide
Synonyms: protein disulfide isomerase, Pdia1, Thbp, PDI, ERp59
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18453
HGNC: HGNC:8548
Homologene: 55495
Adam19
Name: ADAM metallopeptidase domain 19
Synonyms: Mltnb
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11492
HGNC: HGNC:197
Homologene: 74925
Supt7l
Name: SPT7-like, STAGA complex gamma subunit
Synonyms: 2610524B01Rik, 6030455L14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72195
Homologene: 8907
Usp42
Name: ubiquitin specific peptidase 42
Synonyms: 2410140K03Rik, D5Ertd591e, A630018G05Rik, 3110031A07Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 76800
Homologene: 35425
Klhl2
Name: kelch-like 2, Mayven
Synonyms: Mav, ABP-KELCH, 6030411N21Rik, 8530402H02Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 77113
HGNC: HGNC:6353
Homologene: 21416
Rundc1
Name: RUN domain containing 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217201
Homologene: 15095
Igfbp7
Name: insulin-like growth factor binding protein 7
Synonyms: Fstl2, AGM, mac25
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 29817
HGNC: HGNC:5476
Homologene: 1193
Klb
Name: klotho beta
Synonyms: betaKlotho
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 83379
Homologene: 12820
Col27a1
Name: collagen, type XXVII, alpha 1
Synonyms: 5730512J02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 373864
Homologene: 69400
Or10g9b
Name: olfactory receptor family 10 subfamily G member 9B
Synonyms: Olfr980, MOR223-2, GA_x6K02T2PVTD-33705428-33704496
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 259110
VEGA: 9
Homologene: 81557
Klhdc8a
Name: kelch domain containing 8A
Synonyms: A630065K24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 213417
Homologene: 10069
Fgd5
Name: FYVE, RhoGEF and PH domain containing 5
Synonyms: ZFYVE23, C330025N11Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232237
Homologene: 27798
Col6a6
Name: collagen, type VI, alpha 6
Synonyms: E330026B02Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 245026
Homologene: 18260
Or51ab3
Name: olfactory receptor family 51 subfamily AB member 3
Synonyms: MOR20-1, GA_x6K02T2PBJ9-6271959-6272393, GA_x6K02T2PBJ9-6275524-6276477, Olfr614, MOR20-1, Olfr613
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259104
Homologene: 17505
Ptprn
Name: protein tyrosine phosphatase receptor type N
Synonyms: IA-2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19275
HGNC: HGNC:9676
Homologene: 48142
Sppl2c
Name: signal peptide peptidase 2C
Synonyms: 4933407P14Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237958
Homologene: 18491
Plekhh1
Name: pleckstrin homology domain containing, family H (with MyTH4 domain) member 1
Synonyms: D630024D12Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 211945
VEGA: 12
Homologene: 121939
Emcn
Name: endomucin
Synonyms: 0610012K22Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 59308
Homologene: 9438
Or4c111
Name: olfactory receptor family 4 subfamily C member 111
Synonyms: MOR233-9, Olfr1216, GA_x6K02T2Q125-50494588-50493653
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258895
Homologene: 74241
Col11a1
Name: collagen, type XI, alpha 1
Synonyms: C530001D20Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 12814
HGNC: HGNC:2186
Homologene: 56389
Or1x2
Name: olfactory receptor family 1 subfamily X member 2
Synonyms: GA_x6K02T2QP88-4411197-4410256, Olfr54, MOR126-1, F3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18354
Homologene: 115479
Or8b56
Name: olfactory receptor family 8 subfamily B member 56
Synonyms: GA_x6K02T2PVTD-32530445-32531380, Olfr923, MOR164-2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258812
VEGA: 9
Jade2
Name: jade family PHD finger 2
Synonyms: 1200017K05Rik, Phf15
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76901
Homologene: 9082
Ugt1a1
Name: UDP glucuronosyltransferase 1 family, polypeptide A1
Synonyms: UgtBr1, UGT1A01, Udpgt-1a, Gnt1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 394436
Homologene: 128034
Nacad
Name: NAC alpha domain containing
Synonyms: mKIAA0363
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 192950
Homologene: 135717
Musk
Name: muscle, skeletal, receptor tyrosine kinase
Synonyms: Nsk2, Nsk3, MDK4, Nsk1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18198
HGNC: HGNC:7525
Homologene: 4084
Cabyr
Name: calcium binding tyrosine phosphorylation regulated
Synonyms: 1700016C01Rik, CBP86, FSP-2, 4933421A18Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 71132
Homologene: 49299
Sema6c
Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C
Synonyms: Sema Y, Semay
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20360
Homologene: 7931
E330034G19Rik
Name: RIKEN cDNA E330034G19 gene
Synonyms: ZPAC
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105418
Trim38
Name: tripartite motif-containing 38
Synonyms: LOC214158
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 214158
Homologene: 48427
Plat
Name: plasminogen activator, tissue
Synonyms: tPA, D8Ertd2e, t-PA
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18791
HGNC: HGNC:9051
Homologene: 717
Usp18
Name: ubiquitin specific peptidase 18
Synonyms: UBP43, 1110058H21Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 24110
Homologene: 8047
Prss21
Name: serine protease 21
Synonyms: TESP5, testisin, 1700023E12Rik, mT4
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 57256
HGNC: HGNC:9485
Homologene: 137342
Mfsd8
Name: major facilitator superfamily domain containing 8
Synonyms: Cln7, 2810423E13Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 72175
Homologene: 115814
Ivd
Name: isovaleryl coenzyme A dehydrogenase
Synonyms: 1300016K07Rik, 6720455E18Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56357
HGNC: HGNC:6186
Homologene: 1676
Oasl1
Name: 2'-5' oligoadenylate synthetase-like 1
Synonyms: 7530414C13Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231655
HGNC: HGNC:8090
Homologene: 2769
Clec4a1
Name: C-type lectin domain family 4, member a1
Synonyms: mDcir4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 269799
Homologene: 72399
BC030500
Name: cDNA sequence BC030500
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234290
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 75,252,535 bp
  • CAGAGAGAGAGAGA to CAGAGAGAGAGA, chromosome 1 at 88,211,984 bp
  • C to T, chromosome 1 at 132,302,566 bp
  • T to C, chromosome 1 at 193,114,629 bp
  • T to A, chromosome 2 at 89,013,707 bp
  • A to G, chromosome 2 at 118,871,537 bp
  • G to A, chromosome 3 at 40,835,193 bp
  • A to G, chromosome 3 at 95,172,605 bp
  • C to T, chromosome 3 at 114,061,492 bp
  • T to C, chromosome 3 at 137,391,650 bp
  • G to A, chromosome 4 at 58,293,600 bp
  • A to G, chromosome 4 at 63,225,883 bp
  • A to G, chromosome 4 at 132,551,464 bp
  • T to C, chromosome 4 at 149,204,267 bp
  • T to C, chromosome 5 at 31,515,669 bp
  • A to G, chromosome 5 at 65,378,995 bp
  • T to A, chromosome 5 at 77,401,492 bp
  • G to T, chromosome 5 at 100,770,482 bp
  • T to C, chromosome 5 at 114,928,161 bp
  • T to C, chromosome 5 at 143,715,094 bp
  • C to T, chromosome 6 at 91,989,023 bp
  • G to A, chromosome 6 at 121,269,144 bp
  • T to C, chromosome 6 at 122,924,819 bp
  • G to T, chromosome 7 at 46,786,642 bp
  • A to T, chromosome 7 at 103,551,998 bp
  • G to T, chromosome 8 at 22,772,232 bp
  • T to C, chromosome 8 at 58,912,354 bp
  • T to A, chromosome 8 at 64,749,050 bp
  • A to G, chromosome 9 at 38,828,507 bp
  • A to T, chromosome 9 at 40,006,188 bp
  • T to C, chromosome 9 at 105,699,269 bp
  • C to T, chromosome 11 at 6,602,643 bp
  • T to A, chromosome 11 at 28,921,690 bp
  • T to A, chromosome 11 at 46,125,082 bp
  • C to T, chromosome 11 at 51,027,311 bp
  • T to C, chromosome 11 at 51,817,218 bp
  • A to G, chromosome 11 at 82,812,795 bp
  • A to G, chromosome 11 at 101,425,558 bp
  • A to G, chromosome 11 at 104,187,861 bp
  • A to G, chromosome 11 at 109,396,413 bp
  • T to C, chromosome 11 at 120,563,380 bp
  • G to A, chromosome 12 at 79,079,069 bp
  • T to A, chromosome 12 at 111,647,522 bp
  • T to A, chromosome 13 at 23,791,395 bp
  • T to C, chromosome 14 at 24,308,292 bp
  • C to T, chromosome 16 at 23,968,133 bp
  • A to G, chromosome 17 at 23,869,402 bp
  • G to T, chromosome 18 at 12,750,702 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8243 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067648-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.