Strain Name:
C57BL/6J-MtgxR8285Btlr/Mmmh
Stock Number:
067653-MU
Citation ID:
RRID:MMRRC_067653-MU
Other Names:
R8285 (G1)
Major Collection:

Strain Information

Xrcc1
Name: X-ray repair complementing defective repair in Chinese hamster cells 1
Synonyms: Xrcc-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22594
Homologene: 31368
Gad2
Name: glutamic acid decarboxylase 2
Synonyms: GAD65, 6330404F12Rik, GAD(65), Gad-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14417
HGNC: HGNC:4093
Homologene: 20223
Bcar3
Name: breast cancer anti-estrogen resistance 3
Synonyms: AND-34
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 29815
HGNC: HGNC:973
Homologene: 31181
Mapt
Name: microtubule-associated protein tau
Synonyms: Mtapt, Tau
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17762
HGNC: HGNC:6893
Homologene: 74962
Dgkh
Name: diacylglycerol kinase, eta
Synonyms: 5930402B05Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 380921
VEGA: 14
HGNC: HGNC:2854
Homologene: 99373
Kif13b
Name: kinesin family member 13B
Synonyms: N-3 kinesin, C130021D12Rik, 5330429L19Rik, GAKIN
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 16554
VEGA: 14
Homologene: 9073
Scaf8
Name: SR-related CTD-associated factor 8
Synonyms: A930036P18Rik, Rbm16, A630086M08Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 106583
VEGA: 17
Homologene: 8928
Supt16
Name: SPT16, facilitates chromatin remodeling subunit
Synonyms: Spt16, Fact140, Cdc68, Supt16h
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 114741
VEGA: 14
Homologene: 5207
Fan1
Name: FANCD2/FANCI-associated nuclease 1
Synonyms: 6030441H18Rik, Mtmr15
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330554
Homologene: 45598
Muc16
Name: mucin 16
Synonyms: 1110008I14Rik, LOC385009, Gm21044
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 73732
Homologene: 141193
Hspg2
Name: perlecan (heparan sulfate proteoglycan 2)
Synonyms: perlecan, per, Pcn, Plc
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 15530
HGNC: HGNC:5273
Homologene: 68473
Ppp3ca
Name: protein phosphatase 3, catalytic subunit, alpha isoform
Synonyms: CN, CnA, PP2B alpha 1, PP2BA alpha, Caln, 2900074D19Rik, Calna
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 19055
HGNC: HGNC:9314
Homologene: 55497
Atp5pf
Name: ATP synthase peripheral stalk subunit F6
Synonyms: Atp5j
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 11957
HGNC: HGNC:847
Homologene: 1272
Scpep1
Name: serine carboxypeptidase 1
Synonyms: 2410018F01Rik, 4833411K15Rik, Risc
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74617
Homologene: 41443
Fchsd2
Name: FCH and double SH3 domains 2
Synonyms: Sh3md3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 207278
Homologene: 8887
Usf3
Name: upstream transcription factor family member 3
Synonyms: LOC385650, 5530400K22Rik, LOC207806, Gm608
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 207806
Homologene: 19504
Zfhx4
Name: zinc finger homeodomain 4
Synonyms: C130041O22Rik, Zfh4, Zfh-4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80892
Homologene: 23477
Itga11
Name: integrin alpha 11
Synonyms: 4732459H24Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 319480
HGNC: HGNC:6136
Homologene: 8151
Ano4
Name: anoctamin 4
Synonyms: A330096O15Rik, Tmem16d
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 320091
Homologene: 66599
Gm12695
Name: predicted gene 12695
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 620779
Homologene: 51847
Inhbc
Name: inhibin beta-C
Synonyms: activin beta-C
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16325
VEGA: 10
HGNC: HGNC:6068
Homologene: 21142
Maz
Name: MYC-associated zinc finger protein (purine-binding transcription factor)
Synonyms: SAF-2, PUR1, SAF-1, Pur-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17188
HGNC: HGNC:6914
Homologene: 106451
Pramel25
Name: PRAME like 25
Synonyms: Gm13023, MGC:91194
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 194227
Homologene: 103830
Sorbs2
Name: sorbin and SH3 domain containing 2
Synonyms: 2010203O03Rik, 9430041O17Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234214
Homologene: 83295
Mpo
Name: myeloperoxidase
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17523
HGNC: HGNC:7218
Homologene: 55450
Gucy1b2
Name: guanylate cyclase 1, soluble, beta 2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239134
HGNC: HGNC:4686
Homologene: 136630
Rab39
Name: RAB39, member RAS oncogene family
Synonyms: C230094F14Rik, Rab39a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270160
Homologene: 56773
Rgs6
Name: regulator of G-protein signaling 6
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 50779
Homologene: 68385
Or5h26
Name: olfactory receptor family 5 subfamily H member 26
Synonyms: MOR183-1, GA_x54KRFPKG5P-55389051-55388122, Olfr196
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 258775
Homologene: 74113
Or4k47
Name: olfactory receptor family 4 subfamily K member 47
Synonyms: GA_x6K02T2Q125-72673494-72672556, MOR248-4, Olfr1297
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258890
Homologene: 115545
Igsf23
Name: immunoglobulin superfamily, member 23
Synonyms: 2210010C17Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 70080
Homologene: 52756
Ddi1
Name: DNA-damage inducible 1
Synonyms: 1700011N24Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71829
VEGA: 9
Homologene: 100549
Or5t9
Name: olfactory receptor family 5 subfamily T member 9
Synonyms: GA_x6K02T2Q125-48321457-48322449, MOR179-7, Olfr1094
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258362
Homologene: 133609
Mfsd8
Name: major facilitator superfamily domain containing 8
Synonyms: 2810423E13Rik, Cln7
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 72175
Homologene: 115814
Lce1f
Name: late cornified envelope 1F
Synonyms: 1110055J05Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67828
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 2 at 22,624,928 bp
  • T to C, chromosome 2 at 86,829,099 bp
  • A to T, chromosome 2 at 111,622,045 bp
  • C to T, chromosome 3 at 5,401,856 bp
  • G to A, chromosome 3 at 40,835,193 bp
  • T to C, chromosome 3 at 92,718,931 bp
  • A to T, chromosome 3 at 122,512,734 bp
  • A to G, chromosome 3 at 136,881,444 bp
  • G to A, chromosome 4 at 96,769,753 bp
  • A to G, chromosome 4 at 137,512,663 bp
  • T to A, chromosome 4 at 143,794,066 bp
  • T to C, chromosome 7 at 19,941,956 bp
  • G to A, chromosome 7 at 24,572,278 bp
  • A to T, chromosome 7 at 64,366,600 bp
  • T to C, chromosome 7 at 101,233,921 bp
  • T to C, chromosome 7 at 127,025,472 bp
  • T to C, chromosome 8 at 45,796,067 bp
  • T to A, chromosome 9 at 6,265,808 bp
  • G to A, chromosome 9 at 18,642,977 bp
  • A to T, chromosome 9 at 53,705,931 bp
  • T to C, chromosome 9 at 62,752,258 bp
  • A to G, chromosome 10 at 89,068,217 bp
  • T to C, chromosome 10 at 127,370,141 bp
  • A to G, chromosome 11 at 87,797,567 bp
  • A to G, chromosome 11 at 88,952,467 bp
  • T to A, chromosome 11 at 104,298,802 bp
  • A to G, chromosome 12 at 83,116,175 bp
  • A to T, chromosome 14 at 52,181,083 bp
  • A to G, chromosome 14 at 62,420,107 bp
  • A to G, chromosome 14 at 64,782,376 bp
  • T to C, chromosome 14 at 78,628,126 bp
  • T to C, chromosome 16 at 44,220,844 bp
  • G to A, chromosome 16 at 59,167,813 bp
  • A to G, chromosome 16 at 84,828,502 bp
  • T to C, chromosome 17 at 3,177,129 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8285 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067653-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.