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Strain Name:
C57BL/6J-MtgxR8213Btlr/Mmmh
Stock Number:
067655-MU
Citation ID:
RRID:MMRRC_067655-MU
Other Names:
R8213 (G1)
Major Collection:

Strain Information

Dicer1
Name: dicer 1, ribonuclease type III
Synonyms: 1110006F08Rik, D12Ertd7e, Dicer1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 192119
VEGA: 12
Homologene: 13251
Psen2
Name: presenilin 2
Synonyms: ALG-3, PS-2, PS2, Ad4h
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19165
HGNC: HGNC:9509
Homologene: 386
Thbs4
Name: thrombospondin 4
Synonyms: TSP-4, TSP4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 21828
Homologene: 20691
Cdk11b
Name: cyclin dependent kinase 11B
Synonyms: CDK11-p110, PITSLRE proteins, CDK11-p46, CDK11-p58, Cdc2l2, Cdc2l1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12537
Homologene: 22416
Sgsm1
Name: small G protein signaling modulator 1
Synonyms: 2410098H20Rik, D5Bwg1524e, Rutbc2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 52850
Homologene: 64485
Dock6
Name: dedicator of cytokinesis 6
Synonyms: 2410095B20Rik, 4931431C02Rik, C330023D02Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 319899
VEGA: 9
Homologene: 83291
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 37,128,939 bp
  • A to C, chromosome 1 at 180,245,691 bp
  • T to C, chromosome 1 at 192,986,829 bp
  • G to A, chromosome 2 at 25,566,356 bp
  • T to A, chromosome 2 at 67,476,866 bp
  • T to A, chromosome 2 at 85,721,501 bp
  • A to G, chromosome 2 at 121,101,064 bp
  • T to A, chromosome 2 at 150,619,710 bp
  • A to T, chromosome 4 at 25,207,113 bp
  • A to T, chromosome 4 at 48,208,783 bp
  • A to T, chromosome 4 at 129,221,459 bp
  • C to A, chromosome 4 at 132,498,053 bp
  • A to G, chromosome 4 at 136,317,175 bp
  • T to A, chromosome 4 at 143,654,185 bp
  • A to T, chromosome 4 at 144,330,460 bp
  • G to A, chromosome 4 at 155,639,881 bp
  • A to T, chromosome 4 at 156,036,234 bp
  • C to T, chromosome 5 at 32,937,637 bp
  • A to T, chromosome 5 at 113,251,011 bp
  • C to T, chromosome 5 at 143,914,771 bp
  • T to C, chromosome 6 at 35,225,203 bp
  • A to G, chromosome 6 at 83,054,607 bp
  • T to C, chromosome 6 at 120,473,034 bp
  • C to T, chromosome 7 at 6,233,947 bp
  • T to C, chromosome 7 at 32,209,408 bp
  • C to A, chromosome 7 at 108,868,519 bp
  • A to G, chromosome 7 at 128,679,805 bp
  • G to T, chromosome 8 at 91,124,866 bp
  • C to A, chromosome 8 at 129,178,291 bp
  • A to G, chromosome 9 at 4,331,269 bp
  • A to G, chromosome 9 at 21,831,444 bp
  • G to T, chromosome 9 at 53,570,570 bp
  • G to T, chromosome 9 at 66,450,888 bp
  • C to A, chromosome 9 at 79,818,092 bp
  • A to T, chromosome 10 at 52,391,759 bp
  • A to T, chromosome 10 at 94,044,052 bp
  • A to T, chromosome 10 at 127,008,321 bp
  • T to A, chromosome 11 at 72,444,721 bp
  • T to C, chromosome 11 at 73,254,251 bp
  • T to C, chromosome 11 at 77,116,071 bp
  • A to T, chromosome 12 at 44,207,133 bp
  • G to A, chromosome 12 at 51,891,443 bp
  • C to A, chromosome 12 at 55,722,914 bp
  • G to A, chromosome 12 at 100,131,003 bp
  • T to C, chromosome 12 at 103,628,277 bp
  • A to T, chromosome 12 at 104,702,693 bp
  • T to C, chromosome 13 at 23,099,525 bp
  • T to G, chromosome 13 at 28,023,386 bp
  • T to A, chromosome 13 at 32,842,999 bp
  • T to C, chromosome 13 at 92,760,586 bp
  • T to G, chromosome 14 at 61,311,265 bp
  • C to T, chromosome 15 at 10,329,242 bp
  • A to G, chromosome 15 at 59,321,302 bp
  • T to C, chromosome 15 at 60,919,756 bp
  • A to T, chromosome 16 at 96,372,988 bp
  • T to A, chromosome 16 at 97,807,060 bp
  • G to A, chromosome 17 at 20,470,088 bp
  • A to T, chromosome 17 at 34,508,883 bp
  • T to A, chromosome 17 at 80,275,156 bp
  • T to A, chromosome 18 at 23,960,722 bp
  • A to T, chromosome 18 at 61,220,696 bp
  • A to C, chromosome 19 at 3,885,882 bp
  • A to T, chromosome 19 at 8,798,257 bp
  • A to G, chromosome 19 at 12,059,092 bp
  • T to A, chromosome Y at 941,515 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8213 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067655-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.