Strain Name:
C57BL/6J-MtgxR8214Btlr/Mmmh
Stock Number:
067656-MU
Citation ID:
RRID:MMRRC_067656-MU
Other Names:
R8214 (G1)
Major Collection:

Strain Information

Madcam1
Name: mucosal vascular addressin cell adhesion molecule 1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17123
VEGA: 10
HGNC: HGNC:6765
Homologene: 8413
Wdr70
Name: WD repeat domain 70
Synonyms: 4833422F06Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 545085
VEGA: 15
Homologene: 9972
Brd4
Name: bromodomain containing 4
Synonyms: MCAP, HUNK1, WI-11513
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 57261
Homologene: 136213
Ltn1
Name: listerin E3 ubiquitin protein ligase 1
Synonyms: 4930528H02Rik, Zfp294, Listerin, Rnf160
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 78913
VEGA: 16
Homologene: 32272
Bzw1
Name: basic leucine zipper and W2 domains 1
Synonyms: 1200015E15Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 66882
Homologene: 41651
Tut4
Name: terminal uridylyl transferase 4
Synonyms: 6030404K05Rik, 9230115F04Rik, Tent3a, Zcchc11
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230594
Homologene: 35279
Abcc4
Name: ATP-binding cassette, sub-family C member 4
Synonyms: D630049P08Rik, MOAT-B, MRP4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239273
VEGA: 14
HGNC: HGNC:55
Homologene: 74563
Slc12a2
Name: solute carrier family 12, member 2
Synonyms: sodium/potassium/chloride cotransporters, mBSC2, Nkcc1, sy-ns
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 20496
VEGA: 18
Homologene: 20283
Tg
Name: thyroglobulin
Synonyms: Tgn
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 21819
Homologene: 2430
Polr3f
Name: polymerase (RNA) III (DNA directed) polypeptide F
Synonyms: 3110032A07Rik, 2810411G20Rik, RPC39, RPC6, 3010019O03Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 70408
Homologene: 4722
Tomm70a
Name: translocase of outer mitochondrial membrane 70A
Synonyms: D16Ium22, D16Ium22e, 2610044B22Rik, Tomm70a, D16Wsu109e, Tom70
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 28185
VEGA: 16
Homologene: 40112
Ankrd27
Name: ankyrin repeat domain 27
Synonyms: D330003H11Rik, Varp
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 245886
Homologene: 12956
Tenm4
Name: teneurin transmembrane protein 4
Synonyms: l(7)-3Rn, Ten-m4, Doc4, l7Rn3, ELM2, Odz4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 23966
Homologene: 8034
Lpl
Name: lipoprotein lipase
Synonyms: O 1-4-5
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 16956
HGNC: HGNC:6677
Homologene: 200
Efcab2
Name: EF-hand calcium binding domain 2
Synonyms: D830011E08Rik, 1700073K01Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 68226
Homologene: 12248
Muc5ac
Name: mucin 5, subtypes A and C, tracheobronchial/gastric
Synonyms: MGM, 2210005L13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17833
HGNC: HGNC:7515
Homologene: 137237
Dnah6
Name: dynein, axonemal, heavy chain 6
Synonyms: A730004I20Rik, Dnahc6
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330355
HGNC: HGNC:2951
Homologene: 15221
Kidins220
Name: kinase D-interacting substrate 220
Synonyms: 3110039L19Rik, C330002I19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 77480
VEGA: 12
Homologene: 14254
Skint5
Name: selection and upkeep of intraepithelial T cells 5
Synonyms: OTTMUSG00000008560
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242627
Homologene: 135888
Pcdhb5
Name: protocadherin beta 5
Synonyms: Pcdhb4A, PcdhbE
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93876
HGNC: HGNC:8689
Homologene: 75103
Atg4b
Name: autophagy related 4B, cysteine peptidase
Synonyms: Apg4b, 2510009N07Rik, autophagin 1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 66615
Homologene: 100868
Top2b
Name: topoisomerase (DNA) II beta
Synonyms: Top-2, D230016L12Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 21974
Homologene: 134711
Vmn2r105
Name: vomeronasal 2, receptor 105
Synonyms: EG627743
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 627743
Homologene: 129680
Kctd21
Name: potassium channel tetramerisation domain containing 21
Synonyms: EG622320
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 622320
Homologene: 54754
Nrg2
Name: neuregulin 2
Synonyms: NTAK, Don1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 100042150
HGNC: HGNC:7998
Homologene: 75024
Unc45b
Name: unc-45 myosin chaperone B
Synonyms: UNC45, D230041A13Rik, Cmya4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217012
Homologene: 14666
Abcc3
Name: ATP-binding cassette, sub-family C member 3
Synonyms: 1700019L09Rik, MRP3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76408
HGNC: HGNC:54
Homologene: 68364
Or10ak11
Name: olfactory receptor family 10 subfamily AK member 11
Synonyms: GA_x6K02T2QD9B-18703033-18703986, MOR259-11, MOR259-6, Olfr1333
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 258265
Homologene: 134082
Or5p54
Name: olfactory receptor family 5 subfamily P member 54
Synonyms: GA_x6K02T2PBJ9-10283869-10284801, MOR204-20, Olfr474
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258488
Zfp184
Name: zinc finger protein 184 (Kruppel-like)
Synonyms: 4930500C15Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 193452
Homologene: 113602
Vmn1r189
Name: vomeronasal 1 receptor 189
Synonyms: V1rh2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 252906
Homologene: 110880
Zscan5b
Name: zinc finger and SCAN domain containing 5B
Synonyms: Zfg1, Zfp371
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 170734
Homologene: 129555
4930438A08Rik
Name: RIKEN cDNA 4930438A08 gene
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 73988
Homologene: 104884
Vmn1r16
Name: vomeronasal 1 receptor 16
Synonyms: V1rc29
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 171202
Homologene: 123821
Sult2a4
Name: sulfotransferase family 2A, dehydroepiandrosterone (DHEA)-preferring, member 4
Synonyms: Gm5584
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 434121
Homologene: 37741
Abhd16b
Name: abhydrolase domain containing 16B
Synonyms: BC050777
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241850
Homologene: 15422
Or52e2
Name: olfactory receptor family 52 subfamily E member 2
Synonyms: GA_x6K02T2PBJ9-5871256-5870303, MOR32-3, Olfr589
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259054
Homologene: 133046
Lrrc16a
Name:
Type: Gene
Species: Mouse
Chromosome: 13
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 58,405,037 bp
  • A to T, chromosome 1 at 75,315,998 bp
  • T to C, chromosome 1 at 93,784,887 bp
  • T to C, chromosome 1 at 178,437,450 bp
  • A to G, chromosome 2 at 144,536,310 bp
  • C to T, chromosome 2 at 181,494,190 bp
  • A to G, chromosome 4 at 108,512,150 bp
  • T to C, chromosome 4 at 113,804,942 bp
  • A to T, chromosome 4 at 118,830,091 bp
  • T to C, chromosome 6 at 57,323,439 bp
  • T to C, chromosome 6 at 73,044,728 bp
  • C to T, chromosome 7 at 6,233,947 bp
  • A to G, chromosome 7 at 13,989,476 bp
  • A to G, chromosome 7 at 35,614,519 bp
  • T to C, chromosome 7 at 96,895,407 bp
  • T to C, chromosome 7 at 97,347,341 bp
  • A to C, chromosome 7 at 103,155,406 bp
  • T to A, chromosome 7 at 107,954,967 bp
  • A to G, chromosome 7 at 141,802,948 bp
  • T to A, chromosome 8 at 68,892,605 bp
  • A to G, chromosome 10 at 79,666,758 bp
  • G to A, chromosome 11 at 58,286,621 bp
  • A to G, chromosome 11 at 82,933,888 bp
  • C to T, chromosome 11 at 94,363,518 bp
  • C to T, chromosome 12 at 24,994,855 bp
  • A to G, chromosome 13 at 19,104,298 bp
  • T to A, chromosome 13 at 21,958,825 bp
  • C to T, chromosome 13 at 22,102,131 bp
  • T to C, chromosome 13 at 24,044,232 bp
  • G to A, chromosome 14 at 16,383,177 bp
  • T to C, chromosome 14 at 118,500,841 bp
  • G to A, chromosome 15 at 7,887,370 bp
  • C to T, chromosome 15 at 66,773,398 bp
  • A to G, chromosome 15 at 76,192,284 bp
  • G to T, chromosome 16 at 57,121,967 bp
  • A to G, chromosome 16 at 87,380,803 bp
  • T to G, chromosome 17 at 20,228,513 bp
  • A to G, chromosome 17 at 32,212,947 bp
  • T to C, chromosome 18 at 36,196,676 bp
  • G to T, chromosome 18 at 37,321,583 bp
  • A to G, chromosome 18 at 57,937,719 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8214 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067656-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.