Strain Name:
C57BL/6J-MtgxR8224Btlr/Mmmh
Stock Number:
067660-MU
Citation ID:
RRID:MMRRC_067660-MU
Other Names:
R8224 (G1)
Major Collection:

Strain Information

Pax3
Name: paired box 3
Synonyms: Pax-3, Splchl2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18505
HGNC: HGNC:8617
Homologene: 22494
Card11
Name: caspase recruitment domain family, member 11
Synonyms: CARMA1, BIMP3, 2410011D02Rik, 0610008L17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 108723
Homologene: 13024
Gon4l
Name: gon-4 like
Synonyms: 1500041I23Rik, 2610100B20Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76022
Homologene: 13002
Jmjd1c
Name: jumonji domain containing 1C
Synonyms: TRIP8, 5430433L24Rik, D630035I23Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 108829
Homologene: 3129
Yes1
Name: YES proto-oncogene 1, Src family tyrosine kinase
Synonyms: Yes
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22612
Homologene: 55900
Ptpn12
Name: protein tyrosine phosphatase, non-receptor type 12
Synonyms: P19-PTP, PTP-PEST, PTP-P19
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19248
HGNC: HGNC:9645
Homologene: 37691
Kif16b
Name: kinesin family member 16B
Synonyms: N-3 kinesin, 8430434E15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16558
Homologene: 135708
Kmt2a
Name: lysine (K)-specific methyltransferase 2A
Synonyms: trithorax Drosophila, HTRX1, ALL-1, All1, Cxxc7, Mll, Mll1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 214162
HGNC: HGNC:7132
Homologene: 4338
Cenpa
Name: centromere protein A
Synonyms: centrosomin A, Cenp-A
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12615
HGNC: HGNC:1851
Homologene: 1369
Il7
Name: interleukin 7
Synonyms: Il-7, hlb368, A630026I06Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 16196
HGNC: HGNC:6023
Homologene: 680
Xpot
Name: exportin, tRNA (nuclear export receptor for tRNAs)
Synonyms: EXPORTIN-T, C79645, 1110004L07Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 73192
VEGA: 10
Homologene: 38291
Reg4
Name: regenerating islet-derived family, member 4
Synonyms: RELP, 2010002L15Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67709
Homologene: 41671
Ankrd26
Name: ankyrin repeat domain 26
Synonyms: 5730521P14Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232339
Homologene: 45968
Pias4
Name: protein inhibitor of activated STAT 4
Synonyms: PIASy
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 59004
Homologene: 104136
Phlpp1
Name: PH domain and leucine rich repeat protein phosphatase 1
Synonyms: Plekhe1, Phlpp
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98432
Homologene: 11015
Tnip2
Name: TNFAIP3 interacting protein 2
Synonyms: ABIN-2, 1810020H16Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231130
Homologene: 11515
Ppp1r12b
Name: protein phosphatase 1, regulatory subunit 12B
Synonyms: 1810037O03Rik, 9530009M10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 329251
HGNC: HGNC:7619
Homologene: 135710
Cubn
Name: cubilin
Synonyms: D2Wsu88e, intrinsic factor-cobalamin receptor
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 65969
HGNC: HGNC:2548
Homologene: 37434
Otop1
Name: otopetrin 1
Synonyms: A530025J20Rik, tlt
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 21906
Homologene: 35244
Zscan10
Name: zinc finger and SCAN domain containing 10
Synonyms: Zscan10, Zfp206
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 332221
Homologene: 13122
Cdh7
Name: cadherin 7, type 2
Synonyms: CDH7L1, 9330156F07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241201
HGNC: HGNC:1766
Homologene: 68391
Uroc1
Name: urocanase domain containing 1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243537
Homologene: 76629
Col14a1
Name: collagen, type XIV, alpha 1
Synonyms: 5730412L22Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12818
HGNC: HGNC:2191
Homologene: 18741
Adamts9
Name: ADAM metallopeptidase with thrombospondin type 1 motif 9
Synonyms: 1810011L16Rik, E030027K14Rik, 8430403M15Rik, Mhdaund4, Gsfund3, UND3, Mhdaund3, UND4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 101401
Homologene: 18821
Lgi4
Name: leucine-rich repeat LGI family, member 4
Synonyms: clp
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243914
Homologene: 16408
Zfp947
Name: zinc finger protein 947
Synonyms: Gm4769
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210853
Homologene: 133253
Zfp607a
Name: zinc finger protein 607A
Synonyms: 4732475C15Rik, Zfp607
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 545938
Homologene: 134321
Slc25a32
Name: solute carrier family 25, member 32
Synonyms: 2610043O12Rik, Mftc
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 69906
VEGA: 15
Homologene: 6151
Gp1ba
Name: glycoprotein 1b, alpha polypeptide
Synonyms: GP Ib-alpha, GPIb-alpha, GPIba
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14723
HGNC: HGNC:4439
Homologene: 143
Map9
Name: microtubule-associated protein 9
Synonyms: 5033421J10Rik, 5330427D05Rik, ASAP, Mtap9
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 213582
Homologene: 41591
Or6c8
Name: olfactory receptor family 6 subfamily C member 8
Synonyms: GA_x6K02T2PULF-10765431-10764502, MOR115-1, Olfr767
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258315
Homologene: 105186
Zfp932
Name: zinc finger protein 932
Synonyms: 2310001H12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 69504
Homologene: 137770
Nrn1
Name: neuritin 1
Synonyms: 0710008J23Rik, cpg15
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 68404
Homologene: 9577
Pacs2
Name: phosphofurin acidic cluster sorting protein 2
Synonyms: 6720425G15Rik, Pacs1l
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217893
VEGA: 12
Homologene: 15016
9330159F19Rik
Name: RIKEN cDNA 9330159F19 gene
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 212448
VEGA: 10
Homologene: 8804
Cfap61
Name: cilia and flagella associated protein 61
Synonyms: 4930529M08Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 78774
Lrfn5
Name: leucine rich repeat and fibronectin type III domain containing 5
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238205
Homologene: 17582
Gstm2
Name: glutathione S-transferase, mu 2
Synonyms: Gstb-2, Gstb2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14863
Homologene: 121492
Or2d2
Name: olfactory receptor family 2 subfamily D member 2
Synonyms: GA_x6K02T2PBJ9-9479517-9478573, MOR260-1, Olfr715
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258776
HGNC: HGNC:8244
Homologene: 81541
Cst5
Name: cystatin D
Synonyms: DD72, Cst10
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 58214
HGNC: HGNC:2477
Homologene: 55615
Dnajb8
Name: DnaJ heat shock protein family (Hsp40) member B8
Synonyms: mDj6, 1700016F14Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 56691
Homologene: 23184
Ncf2
Name: neutrophil cytosolic factor 2
Synonyms: NADPH oxidase subunit (67kDa), p67phox, Ncf-2, NOXA2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17970
HGNC: HGNC:7661
Homologene: 374
Nlrp4g
Name: NLR family, pyrin domain containing 4G
Synonyms: nalp4g, Gm47182, Gm47180
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235779
VEGA: 9
Insm2
Name: insulinoma-associated 2
Synonyms: mlt 1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 56856
VEGA: 12
Homologene: 10651
Chfr
Name: checkpoint with forkhead and ring finger domains
Synonyms: RNF116, 5730484M20Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231600
Homologene: 10078
Or1ab2
Name: olfactory receptor family 1 subfamily AB member 2
Synonyms: GA_x6K02T2NUPS-241490-242431, MOR130-1, Olfr374
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 258335
Homologene: 131127
Sh3bp5
Name: SH3-domain binding protein 5 (BTK-associated)
Synonyms: Sab
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 24056
Homologene: 23450
Or5h24
Name: olfactory receptor family 5 subfamily H member 24, pseudogene 1
Synonyms: GA_x54KRFPKG5P-55327126-55326203, MOR183-11_p, Olfr192
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 404309
Homologene: 131129
Fam21
Name:
Type: Gene
Species: Mouse
Chromosome: 6
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 78,121,690 bp
  • T to C, chromosome 1 at 106,392,618 bp
  • G to T, chromosome 1 at 109,994,203 bp
  • T to C, chromosome 1 at 134,902,462 bp
  • T to C, chromosome 1 at 152,830,393 bp
  • T to C, chromosome 2 at 13,349,877 bp
  • T to C, chromosome 2 at 142,834,088 bp
  • T to C, chromosome 2 at 145,939,880 bp
  • A to G, chromosome 2 at 149,409,982 bp
  • T to C, chromosome 3 at 7,577,248 bp
  • T to C, chromosome 3 at 82,359,063 bp
  • A to G, chromosome 3 at 88,895,142 bp
  • A to T, chromosome 3 at 98,224,695 bp
  • T to C, chromosome 3 at 107,983,998 bp
  • G to T, chromosome 5 at 20,998,658 bp
  • T to C, chromosome 5 at 30,673,355 bp
  • T to C, chromosome 5 at 32,659,073 bp
  • C to T, chromosome 5 at 34,513,659 bp
  • C to A, chromosome 5 at 38,300,503 bp
  • T to C, chromosome 5 at 109,996,614 bp
  • T to A, chromosome 5 at 110,160,243 bp
  • T to C, chromosome 5 at 140,902,877 bp
  • C to T, chromosome 6 at 88,222,958 bp
  • A to G, chromosome 6 at 90,344,067 bp
  • A to T, chromosome 6 at 92,796,370 bp
  • A to C, chromosome 6 at 116,241,496 bp
  • G to A, chromosome 6 at 118,525,755 bp
  • A to T, chromosome 7 at 27,878,111 bp
  • G to A, chromosome 7 at 31,063,592 bp
  • T to A, chromosome 7 at 107,128,872 bp
  • A to G, chromosome 8 at 72,110,379 bp
  • A to C, chromosome 9 at 44,808,029 bp
  • A to T, chromosome 9 at 124,353,374 bp
  • A to T, chromosome 10 at 29,218,253 bp
  • A to G, chromosome 10 at 67,244,849 bp
  • C to A, chromosome 10 at 81,167,731 bp
  • A to T, chromosome 10 at 121,607,608 bp
  • A to G, chromosome 10 at 129,079,435 bp
  • A to G, chromosome 11 at 70,639,857 bp
  • A to T, chromosome 12 at 55,599,978 bp
  • A to T, chromosome 12 at 61,843,406 bp
  • A to G, chromosome 12 at 113,059,760 bp
  • A to T, chromosome 12 at 115,834,239 bp
  • G to A, chromosome 13 at 36,734,284 bp
  • T to C, chromosome 14 at 31,377,516 bp
  • C to G, chromosome 15 at 39,112,620 bp
  • A to T, chromosome 15 at 55,407,741 bp
  • A to T, chromosome 16 at 59,098,754 bp
  • A to C, chromosome 17 at 22,145,382 bp
  • A to G, chromosome 17 at 23,609,392 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8224 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067660-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.