Strain Name:
C57BL/6J-MtgxR8235Btlr/Mmmh
Stock Number:
067667-MU
Citation ID:
RRID:MMRRC_067667-MU
Other Names:
R8235 (G1)
Major Collection:

Strain Information

Ptk2
Name: PTK2 protein tyrosine kinase 2
Synonyms: FRNK, FAK, Fadk
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14083
VEGA: 15
HGNC: HGNC:9611
Homologene: 7314
Chrna7
Name: cholinergic receptor, nicotinic, alpha polypeptide 7
Synonyms: Acra7, alpha7 nicotinic receptor, alpha7-nAChR, alpha7
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11441
Homologene: 593
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Ptprk
Name: protein tyrosine phosphatase receptor type K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Eps8
Name: epidermal growth factor receptor pathway substrate 8
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13860
HGNC: HGNC:3420
Homologene: 3272
Map3k4
Name: mitogen-activated protein kinase kinase kinase 4
Synonyms: D17Rp17e, Tas, MAPKKK4, D17Rp17, Mekk4, RP17, T-associated sex reversal, MTK1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 26407
VEGA: 17
HGNC: HGNC:6856
Homologene: 31346
Vps13c
Name: vacuolar protein sorting 13C
Synonyms: C230055H22Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 320528
VEGA: 9
Homologene: 41188
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: C130061D10Rik, Phr1, Pam
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105689
Homologene: 9005
Prdm2
Name: PR domain containing 2, with ZNF domain
Synonyms: KMT8, Riz, Riz1, LOC381568, E330024L24Rik, 4833427P12Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 110593
HGNC: HGNC:9347
Homologene: 40822
Samd4b
Name: sterile alpha motif domain containing 4B
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233033
Homologene: 35268
Bptf
Name: bromodomain PHD finger transcription factor
Synonyms: 9430093H17Rik, Falz
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 207165
HGNC: HGNC:3581
Homologene: 114397
Rsf1
Name: remodeling and spacing factor 1
Synonyms: 4832420A03Rik, XAP8, Hbxap, C030033M12Rik, p325
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233532
Homologene: 41142
Chek1
Name: checkpoint kinase 1
Synonyms: Chk1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12649
HGNC: HGNC:1925
Homologene: 975
Ufl1
Name: UFM1 specific ligase 1
Synonyms: Maxer, 1810074P20Rik, Rcad
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67490
Homologene: 9093
Vegfa
Name: vascular endothelial growth factor A
Synonyms: VEGF-A, VEGF120, Vegf, VEGF188, VEGF164, VPF
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22339
Homologene: 2534
Farsa
Name: phenylalanyl-tRNA synthetase, alpha subunit
Synonyms: Farsla, 0610012A19Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 66590
HGNC: HGNC:3592
Homologene: 3280
Ehbp1
Name: EH domain binding protein 1
Synonyms: Flj21950, KIAA0903-like
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216565
Homologene: 22880
Nfkbia
Name: nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha
Synonyms: Nfkbi, I-kappaBalpha, IkappaBalpha, I(Kappa)B(alpha)
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 18035
VEGA: 12
HGNC: HGNC:7797
Homologene: 7863
Tulp3
Name: TUB like protein 3
Synonyms: 2310022L06Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 22158
Homologene: 20711
Abca13
Name: ATP-binding cassette, sub-family A member 13
Synonyms: A930002G16Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 268379
Homologene: 27991
Ptprq
Name: protein tyrosine phosphatase receptor type Q
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237523
HGNC: HGNC:9679
Homologene: 83557
Spta1
Name: spectrin alpha, erythrocytic 1
Synonyms: erythroid, Spna-1, ihj, Spna1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20739
Homologene: 74460
Nwd1
Name: NACHT and WD repeat domain containing 1
Synonyms: A230063L24Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 319555
Homologene: 72261
Myh2
Name: myosin, heavy polypeptide 2, skeletal muscle, adult
Synonyms: MHC2A, Myhs-f, Myhs-f1, Myhsf1, MyHC-IIa
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17882
HGNC: HGNC:7572
Homologene: 23019
Msto1
Name: misato 1, mitochondrial distribution and morphology regulator
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229524
Homologene: 41228
Rbm46
Name: RNA binding motif protein 46
Synonyms: ENSMUSG00000033882, LOC329687
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 633285
Homologene: 17016
Entpd7
Name: ectonucleoside triphosphate diphosphohydrolase 7
Synonyms: Lysal2, 1810020C02Rik, 1810012B13Rik, 2810003F23Rik, LALP1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 93685
Homologene: 122202
Npr2
Name: natriuretic peptide receptor 2
Synonyms: pwe, cn, guanylyl cyclase-B
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230103
HGNC: HGNC:7944
Homologene: 2970
Ccnd2
Name: cyclin D2
Synonyms: Vin1, cD2, 2600016F06Rik, Vin-1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12444
HGNC: HGNC:1583
Homologene: 37525
Or8i2
Name: olfactory receptor family 8 subfamily I member 2
Synonyms: Olfr1104, GA_x6K02T2Q125-48508763-48507833, MOR207-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258763
Homologene: 64916
Unc45b
Name: unc-45 myosin chaperone B
Synonyms: D230041A13Rik, Cmya4, UNC45
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217012
Homologene: 14666
Hpn
Name: hepsin
Synonyms: Hlb320
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 15451
HGNC: HGNC:5155
Homologene: 20498
Sytl1
Name: synaptotagmin-like 1
Synonyms: PSGL-1, Slp1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269589
Homologene: 12853
Abhd13
Name: abhydrolase domain containing 13
Synonyms: 1110065L07Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 68904
Homologene: 6716
Atp8b3
Name: ATPase, class I, type 8B, member 3
Synonyms: 1700042F02Rik, 1700056N23Rik, SAPLT
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67331
VEGA: 10
Homologene: 19034
Trmt10c
Name: tRNA methyltransferase 10C, mitochondrial RNase P subunit
Synonyms: D16Ertd454e, 1300018J16Rik, Rg9mtd1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 52575
Homologene: 9858
Zfp184
Name: zinc finger protein 184 (Kruppel-like)
Synonyms: 4930500C15Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 193452
Homologene: 113602
Cbll1
Name: Casitas B-lineage lymphoma-like 1
Synonyms: c-Cbl-like, Hakai
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 104836
Homologene: 11734
Trpc4
Name: transient receptor potential cation channel, subfamily C, member 4
Synonyms: STRPC4, CCE1, Trp4, Trrp4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 22066
Homologene: 22955
Gm10110
Name: predicted gene 10110
Type: Gene
Species: Mouse
Chromosome: 14
Crybb1
Name: crystallin, beta B1
Synonyms: 3110006K12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12960
HGNC: HGNC:2397
Homologene: 1423
Potefam3e
Name: POTE ankyrin domain family member 3E
Synonyms: TSAP, ENSMUSG00000074453, OTTMUSG00000018948, 4930467E23Rik, Pote3e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 626415
Homologene: 128726
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 174,202,386 bp
  • T to C, chromosome 2 at 87,021,882 bp
  • G to A, chromosome 3 at 54,302,248 bp
  • C to T, chromosome 3 at 82,865,468 bp
  • A to T, chromosome 3 at 88,912,921 bp
  • T to C, chromosome 4 at 25,278,656 bp
  • T to A, chromosome 4 at 43,641,603 bp
  • T to C, chromosome 4 at 133,260,946 bp
  • G to A, chromosome 4 at 143,132,467 bp
  • A to G, chromosome 5 at 112,257,400 bp
  • T to C, chromosome 6 at 127,130,342 bp
  • T to C, chromosome 6 at 128,327,677 bp
  • A to G, chromosome 6 at 137,483,578 bp
  • C to T, chromosome 7 at 28,406,606 bp
  • T to C, chromosome 7 at 31,102,783 bp
  • T to C, chromosome 7 at 63,212,224 bp
  • T to C, chromosome 7 at 97,676,254 bp
  • G to A, chromosome 8 at 9,987,394 bp
  • C to A, chromosome 8 at 19,749,460 bp
  • TCATCC to TCATCCATCC, chromosome 8 at 72,711,686 bp
  • A to G, chromosome 8 at 84,868,916 bp
  • T to A, chromosome 9 at 36,719,574 bp
  • T to C, chromosome 9 at 67,927,396 bp
  • T to A, chromosome 9 at 67,955,781 bp
  • T to A, chromosome 10 at 28,589,041 bp
  • T to C, chromosome 10 at 80,529,816 bp
  • A to C, chromosome 10 at 107,582,541 bp
  • A to T, chromosome 10 at 107,705,490 bp
  • A to C, chromosome 11 at 9,262,077 bp
  • G to A, chromosome 11 at 22,239,153 bp
  • A to G, chromosome 11 at 67,192,998 bp
  • A to G, chromosome 11 at 82,919,855 bp
  • A to G, chromosome 11 at 107,076,632 bp
  • C to T, chromosome 12 at 31,491,571 bp
  • T to C, chromosome 12 at 55,490,823 bp
  • T to A, chromosome 13 at 13,760,738 bp
  • T to G, chromosome 13 at 21,959,883 bp
  • C to T, chromosome 14 at 89,898,241 bp
  • T to C, chromosome 14 at 103,198,674 bp
  • T to C, chromosome 15 at 73,343,291 bp
  • T to C, chromosome 16 at 56,034,576 bp
  • A to T, chromosome 17 at 12,240,081 bp
  • A to T, chromosome 17 at 46,031,310 bp
  • G to A, chromosome 19 at 43,717,545 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8235 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067667-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.