Strain Name:
C57BL/6J-MtgxR8254Btlr/Mmmh
Stock Number:
067680-MU
Citation ID:
RRID:MMRRC_067680-MU
Other Names:
R8254 (G1)
Major Collection:

Strain Information

Dspp
Name: dentin sialophosphoprotein
Synonyms: Dpp, Dsp, Dmp3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 666279
HGNC: HGNC:3054
Ntrk3
Name: neurotrophic tyrosine kinase, receptor, type 3
Synonyms: TrkC
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18213
HGNC: HGNC:8033
Homologene: 49183
Calb1
Name: calbindin 1
Synonyms: Brain-2, CB, Calb, Calb-1, CalbindinD28K, calbindin, calbindin-D (28k)
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12307
HGNC: HGNC:1434
Homologene: 21026
Fam3c
Name: FAM3 metabolism regulating signaling molecule C
Synonyms: Fam3c, Ilei, D6Wsu176e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 27999
Homologene: 8926
Zfp568
Name: zinc finger protein 568
Synonyms: LOC381866, chato
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243905
Homologene: 136307
Thada
Name: thyroid adenoma associated
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240174
VEGA: 17
Homologene: 75175
Map4k4
Name: mitogen-activated protein kinase kinase kinase kinase 4
Synonyms: Nik, 9430080K19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 26921
HGNC: HGNC:6866
Homologene: 7442
Rere
Name: arginine glutamic acid dipeptide (RE) repeats
Synonyms: eyem03Jus, 1110033A15Rik, atrophin-2, eyes3, Atr2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68703
HGNC: HGNC:9965
Homologene: 8101
Cog6
Name: component of oligomeric golgi complex 6
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67542
Homologene: 10802
Kctd2
Name: potassium channel tetramerisation domain containing 2
Synonyms: 2310012I15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 70382
Homologene: 82389
Vps13d
Name: vacuolar protein sorting 13D
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230895
Homologene: 15583
Nrip1
Name: nuclear receptor interacting protein 1
Synonyms: 8430438I05Rik, RIP140, 6030458L20Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 268903
HGNC: HGNC:8001
Homologene: 2606
Zfp846
Name: zinc finger protein 846
Synonyms: 2210010B09Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244721
Homologene: 87243
Kirrel2
Name: kirre like nephrin family adhesion molecule 2
Synonyms: C330019F22Rik, NEPH3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243911
Homologene: 35249
Arhgef2
Name: Rho/Rac guanine nucleotide exchange factor 2
Synonyms: P40, GEF-H1, Lbcl1, Lfc, LFP40, GEFH1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 16800
HGNC: HGNC:682
Homologene: 3468
Tars3
Name: threonyl-tRNA synthetase 3
Synonyms: A530046H20Rik, Tarsl2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 272396
Homologene: 65036
Pi16
Name: peptidase inhibitor 16
Synonyms: 1200009H11Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 74116
Homologene: 134317
Dnah11
Name: dynein, axonemal, heavy chain 11
Synonyms: b2b1289Clo, b2b1203Clo, Dnahc11, b2b1279Clo, avc4, b2b1727Clo, b2b598Clo, lrd
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 13411
HGNC: HGNC:2942
Homologene: 2801
Pgm5
Name: phosphoglucomutase 5
Synonyms: 9530034F03Rik, aciculin
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 226041
HGNC: HGNC:8908
Homologene: 74881
Pcdhb11
Name: protocadherin beta 11
Synonyms: PcdhbK, Pcdhb5E
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93882
HGNC: HGNC:8690
Homologene: 62178
Wdr11
Name: WD repeat domain 11
Synonyms: Wdr11, 2900055P10Rik, Brwd2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 207425
Homologene: 41229
Fcgbpl1
Name: Fc fragment of IgG binding protein like 1
Synonyms: 9530053A07Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 319482
Homologene: 130055
Or5ac25
Name: olfactory receptor family 5 subfamily AC member 25
Synonyms: Olfr209, Olfr207, GA_x54KRFPKG5P-55570276-55569359, MOR182-9, Olfr208, GA_x54KRFPKG5P-55580232-55579315, MOR182-6, MOR182-6, GA_x54KRFPKG5P-55590495-55589578
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 404311
Homologene: 133040
Tubd1
Name: tubulin, delta 1
Synonyms: 4930550G19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56427
Homologene: 9443
Vwce
Name: von Willebrand factor C and EGF domains
Synonyms: 1300015B04Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 71768
VEGA: 19
Homologene: 17651
Sema4f
Name: sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain
Synonyms: Sema W
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20355
Homologene: 3147
Vmn2r72
Name: vomeronasal 2, receptor 72
Synonyms: EG244114, Vmn2r72-ps
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 244114
Homologene: 115466
Cyp4f13
Name: cytochrome P450, family 4, subfamily f, polypeptide 13
Synonyms: P450 CYP4F13, 0610030I10Rik, leukotriene B4 omega hydroxylase
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 170716
HGNC: HGNC:2646
Homologene: 73902
Psd4
Name: pleckstrin and Sec7 domain containing 4
Synonyms: EFA6B, SEC7 homolog
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 215632
Homologene: 8261
Or5b96
Name: olfactory receptor family 5 subfamily B member 96
Synonyms: MOR202-2, GA_x6K02T2RE5P-3220047-3219130, Olfr1446
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258699
HGNC: HGNC:8324
Homologene: 74233
Sorcs2
Name: sortilin-related VPS10 domain containing receptor 2
Synonyms: VPS10 domain receptor protein
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 81840
Homologene: 56899
Gm14226
Name: predicted gene 14226
Type: Gene
Species: Mouse
Chromosome: 2
Itga2b
Name: integrin alpha 2b
Synonyms: platelet glycoprotein IIb, CD41, GP IIb, alphaIIb, GpIIb
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16399
HGNC: HGNC:6138
Homologene: 37304
Grip1
Name: glutamate receptor interacting protein 1
Synonyms: eb, 4931400F03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74053
Homologene: 12938
Fdxr
Name: ferredoxin reductase
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14149
HGNC: HGNC:3642
Homologene: 3033
Sv2c
Name: synaptic vesicle glycoprotein 2c
Synonyms: 4930527L09Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 75209
Homologene: 57152
Brsk2
Name: BR serine/threonine kinase 2
Synonyms: 4833424K13Rik, SAD-A
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 75770
Homologene: 123829
Aadacl3
Name: arylacetamide deacetylase like 3
Synonyms: LOC230883
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230883
Homologene: 28426
Fndc5
Name: fibronectin type III domain containing 5
Synonyms: 1500001L03Rik, Pxp, PeP
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 384061
Homologene: 17812
Ldc1
Name: leucine decarboxylase 1
Synonyms: Gm853, LOC332942
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 332942
Homologene: 128653
Zfp606
Name: zinc finger protein 606
Synonyms: 2410022M24Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67370
Homologene: 23514
Lmx1b
Name: LIM homeobox transcription factor 1 beta
Synonyms: LMX1.2, Icst, GENA 191
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16917
HGNC: HGNC:6654
Homologene: 55648
Gsg2
Name:
Type: Gene
Species: Mouse
Chromosome: 11
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 40,006,675 bp
  • A to T, chromosome 2 at 24,403,211 bp
  • A to G, chromosome 2 at 33,565,114 bp
  • T to C, chromosome 2 at 155,024,726 bp
  • T to C, chromosome 3 at 52,993,517 bp
  • T to C, chromosome 3 at 88,642,603 bp
  • A to G, chromosome 4 at 15,898,926 bp
  • T to C, chromosome 4 at 129,138,721 bp
  • C to A, chromosome 4 at 130,220,343 bp
  • T to C, chromosome 4 at 144,456,190 bp
  • A to T, chromosome 4 at 144,983,312 bp
  • T to C, chromosome 4 at 150,612,672 bp
  • A to G, chromosome 5 at 36,038,206 bp
  • A to T, chromosome 5 at 104,175,328 bp
  • T to A, chromosome 6 at 22,328,676 bp
  • A to T, chromosome 6 at 82,917,401 bp
  • T to C, chromosome 7 at 12,492,861 bp
  • T to C, chromosome 7 at 28,147,349 bp
  • A to T, chromosome 7 at 30,015,133 bp
  • A to G, chromosome 7 at 30,450,376 bp
  • A to G, chromosome 7 at 65,676,061 bp
  • T to C, chromosome 7 at 78,192,578 bp
  • T to C, chromosome 7 at 85,751,019 bp
  • T to C, chromosome 7 at 129,634,836 bp
  • T to C, chromosome 7 at 141,984,416 bp
  • A to G, chromosome 9 at 20,593,291 bp
  • C to T, chromosome 10 at 120,054,905 bp
  • A to T, chromosome 11 at 73,136,746 bp
  • A to T, chromosome 11 at 86,549,438 bp
  • C to A, chromosome 11 at 102,467,386 bp
  • C to A, chromosome 11 at 115,271,318 bp
  • C to T, chromosome 11 at 115,420,348 bp
  • T to C, chromosome 12 at 117,878,524 bp
  • T to C, chromosome 13 at 96,088,565 bp
  • T to A, chromosome 16 at 59,362,171 bp
  • T to C, chromosome 16 at 76,291,399 bp
  • C to A, chromosome 17 at 29,319,234 bp
  • A to T, chromosome 17 at 32,929,933 bp
  • T to C, chromosome 17 at 84,226,040 bp
  • T to C, chromosome 18 at 37,422,189 bp
  • C to A, chromosome 19 at 10,650,574 bp
  • T to C, chromosome 19 at 12,890,485 bp
  • T to C, chromosome 19 at 24,727,725 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8254 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067680-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.