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Strain Name:
C57BL/6J-MtgxR8260Btlr/Mmmh
Stock Number:
067685-MU
Citation ID:
RRID:MMRRC_067685-MU
Other Names:
R8260 (G1)
Major Collection:

Strain Information

Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Cdh1
Name: cadherin 1
Synonyms: E-cadherin, Ecad, UM, uvomorulin, L-CAM, E-cad
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12550
HGNC: HGNC:1748
Homologene: 20917
D630045J12Rik
Name: RIKEN cDNA D630045J12 gene
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330286
Homologene: 19782
Sirt3
Name: sirtuin 3
Synonyms: Sir2l3, 2310003L23Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 64384
Homologene: 81827
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 50,938,319 bp
  • T to A, chromosome 1 at 123,686,295 bp
  • A to T, chromosome 1 at 130,388,678 bp
  • T to C, chromosome 1 at 180,738,530 bp
  • T to C, chromosome 2 at 15,614,206 bp
  • A to G, chromosome 2 at 36,452,885 bp
  • T to C, chromosome 2 at 73,335,858 bp
  • T to C, chromosome 2 at 76,765,993 bp
  • A to T, chromosome 2 at 85,759,476 bp
  • T to A, chromosome 2 at 86,346,932 bp
  • A to T, chromosome 3 at 69,820,972 bp
  • A to G, chromosome 3 at 88,892,630 bp
  • A to G, chromosome 3 at 95,754,820 bp
  • A to G, chromosome 3 at 104,015,309 bp
  • A to G, chromosome 4 at 45,244,638 bp
  • T to A, chromosome 4 at 65,316,182 bp
  • A to G, chromosome 4 at 123,472,070 bp
  • T to A, chromosome 5 at 25,405,516 bp
  • T to A, chromosome 5 at 110,755,612 bp
  • C to T, chromosome 5 at 112,461,256 bp
  • C to T, chromosome 5 at 118,748,729 bp
  • T to C, chromosome 5 at 139,765,213 bp
  • T to A, chromosome 6 at 38,142,911 bp
  • T to C, chromosome 6 at 56,137,419 bp
  • T to C, chromosome 6 at 87,630,005 bp
  • T to A, chromosome 6 at 129,831,458 bp
  • C to T, chromosome 7 at 19,006,154 bp
  • G to A, chromosome 7 at 27,476,359 bp
  • A to G, chromosome 7 at 34,644,842 bp
  • T to C, chromosome 7 at 44,615,376 bp
  • T to C, chromosome 7 at 88,075,132 bp
  • T to A, chromosome 7 at 102,614,225 bp
  • T to A, chromosome 7 at 104,938,672 bp
  • A to T, chromosome 7 at 106,806,027 bp
  • A to T, chromosome 7 at 107,106,855 bp
  • A to T, chromosome 7 at 113,285,051 bp
  • A to G, chromosome 7 at 140,876,406 bp
  • T to C, chromosome 7 at 143,585,709 bp
  • T to C, chromosome 8 at 46,292,708 bp
  • A to T, chromosome 8 at 69,519,748 bp
  • A to T, chromosome 8 at 106,257,415 bp
  • G to A, chromosome 8 at 106,604,347 bp
  • A to T, chromosome 9 at 6,265,524 bp
  • A to G, chromosome 9 at 59,910,678 bp
  • T to A, chromosome 9 at 105,418,579 bp
  • C to A, chromosome 9 at 109,146,546 bp
  • A to T, chromosome 9 at 113,672,797 bp
  • T to A, chromosome 10 at 29,148,274 bp
  • T to C, chromosome 10 at 59,951,455 bp
  • C to T, chromosome 10 at 75,581,411 bp
  • C to T, chromosome 10 at 111,300,243 bp
  • T to A, chromosome 10 at 129,527,088 bp
  • A to T, chromosome 11 at 29,493,934 bp
  • A to T, chromosome 11 at 48,894,729 bp
  • A to G, chromosome 11 at 50,164,186 bp
  • C to A, chromosome 11 at 58,058,689 bp
  • C to T, chromosome 11 at 75,656,116 bp
  • T to A, chromosome 11 at 85,509,546 bp
  • T to A, chromosome 11 at 99,000,769 bp
  • C to A, chromosome 12 at 84,042,035 bp
  • T to C, chromosome 12 at 104,239,103 bp
  • A to G, chromosome 13 at 32,805,356 bp
  • G to T, chromosome 13 at 58,127,414 bp
  • T to A, chromosome 13 at 62,172,738 bp
  • T to C, chromosome 13 at 74,058,346 bp
  • T to C, chromosome 14 at 50,320,158 bp
  • G to C, chromosome 14 at 63,986,184 bp
  • C to A, chromosome 14 at 123,827,834 bp
  • A to T, chromosome 15 at 6,815,416 bp
  • A to G, chromosome 15 at 30,634,733 bp
  • C to A, chromosome 15 at 72,941,909 bp
  • A to G, chromosome 15 at 89,012,110 bp
  • A to G, chromosome 15 at 98,601,038 bp
  • CAC to CACTAC, chromosome 16 at 32,754,076 bp
  • T to C, chromosome 16 at 46,154,423 bp
  • A to G, chromosome 16 at 63,583,554 bp
  • A to T, chromosome 17 at 3,518,319 bp
  • A to G, chromosome 17 at 5,332,513 bp
  • A to G, chromosome 17 at 18,583,981 bp
  • A to T, chromosome 17 at 25,845,167 bp
  • A to G, chromosome 17 at 57,257,314 bp
  • T to A, chromosome 18 at 35,654,157 bp
  • T to C, chromosome 18 at 54,897,749 bp
  • G to A, chromosome 18 at 62,013,066 bp
  • C to T, chromosome 19 at 5,665,384 bp
  • A to G, chromosome 19 at 33,202,648 bp
  • G to C, chromosome X at 101,793,784 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8260 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067685-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.