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Strain Name:
C57BL/6J-MtgxR8261Btlr/Mmmh
Stock Number:
067686-MU
Citation ID:
RRID:MMRRC_067686-MU
Other Names:
R8261 (G1)
Major Collection:

Strain Information

Cdk6
Name: cyclin dependent kinase 6
Synonyms: Crk2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12571
HGNC: HGNC:1777
Homologene: 963
Cntnap2
Name: contactin associated protein-like 2
Synonyms: 5430425M22Rik, Caspr2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 66797
Homologene: 69159
Dicer1
Name: dicer 1, ribonuclease type III
Synonyms: 1110006F08Rik, D12Ertd7e, Dicer1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 192119
VEGA: 12
Homologene: 13251
E2f2
Name: E2F transcription factor 2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242705
HGNC: HGNC:3114
Homologene: 48264
Rapgef2
Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: 5830453M24Rik, Pdzgef1, RA-GEF-1, CNRasGEF, nRapGEP
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76089
Homologene: 35477
Adam23
Name: a disintegrin and metallopeptidase domain 23
Synonyms: MDC3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23792
HGNC: HGNC:202
Homologene: 2826
Chd1
Name: chromodomain helicase DNA binding protein 1
Synonyms: 4930525N21Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12648
HGNC: HGNC:1915
Homologene: 68174
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 63,528,798 bp
  • T to A, chromosome 1 at 91,433,448 bp
  • T to C, chromosome 1 at 135,827,762 bp
  • T to A, chromosome 1 at 136,225,477 bp
  • T to A, chromosome 1 at 194,749,416 bp
  • T to A, chromosome 2 at 18,745,490 bp
  • T to C, chromosome 2 at 29,777,713 bp
  • A to G, chromosome 2 at 76,917,424 bp
  • A to G, chromosome 2 at 89,943,372 bp
  • A to T, chromosome 2 at 120,047,135 bp
  • A to T, chromosome 2 at 151,927,164 bp
  • A to G, chromosome 2 at 160,957,082 bp
  • T to C, chromosome 3 at 25,433,652 bp
  • A to G, chromosome 3 at 28,920,600 bp
  • A to G, chromosome 3 at 30,895,947 bp
  • T to C, chromosome 3 at 40,724,920 bp
  • A to G, chromosome 3 at 79,086,018 bp
  • T to C, chromosome 3 at 87,088,002 bp
  • A to C, chromosome 4 at 86,276,883 bp
  • T to C, chromosome 4 at 109,016,679 bp
  • A to G, chromosome 4 at 126,904,567 bp
  • A to G, chromosome 4 at 136,184,480 bp
  • T to A, chromosome 4 at 138,171,118 bp
  • A to T, chromosome 4 at 139,085,521 bp
  • T to G, chromosome 5 at 3,390,685 bp
  • T to A, chromosome 5 at 45,687,388 bp
  • C to A, chromosome 5 at 143,321,833 bp
  • T to G, chromosome 5 at 143,381,940 bp
  • A to G, chromosome 5 at 150,445,907 bp
  • T to A, chromosome 6 at 43,153,308 bp
  • T to C, chromosome 6 at 47,095,693 bp
  • A to T, chromosome 6 at 57,036,173 bp
  • T to C, chromosome 6 at 67,766,118 bp
  • A to G, chromosome 7 at 34,748,154 bp
  • T to A, chromosome 7 at 42,612,701 bp
  • A to G, chromosome 7 at 46,653,749 bp
  • T to C, chromosome 7 at 80,383,154 bp
  • T to C, chromosome 7 at 105,555,313 bp
  • A to G, chromosome 8 at 64,964,320 bp
  • A to T, chromosome 8 at 84,092,850 bp
  • T to A, chromosome 8 at 104,615,179 bp
  • T to C, chromosome 9 at 42,014,481 bp
  • A to G, chromosome 9 at 57,139,658 bp
  • A to T, chromosome 9 at 67,954,980 bp
  • A to G, chromosome 9 at 121,451,667 bp
  • A to T, chromosome 10 at 116,237,264 bp
  • G to T, chromosome 11 at 5,134,353 bp
  • A to T, chromosome 11 at 73,254,767 bp
  • G to A, chromosome 12 at 61,839,537 bp
  • C to A, chromosome 12 at 76,621,262 bp
  • G to T, chromosome 12 at 103,402,902 bp
  • A to T, chromosome 12 at 104,691,606 bp
  • T to A, chromosome 13 at 19,751,843 bp
  • C to A, chromosome 13 at 55,472,922 bp
  • T to A, chromosome 13 at 67,825,951 bp
  • A to G, chromosome 13 at 95,635,570 bp
  • T to C, chromosome 14 at 33,502,977 bp
  • C to T, chromosome 14 at 42,794,707 bp
  • G to A, chromosome 14 at 52,019,251 bp
  • A to T, chromosome 14 at 59,413,692 bp
  • T to A, chromosome 15 at 58,560,496 bp
  • T to C, chromosome 15 at 60,016,576 bp
  • A to T, chromosome 15 at 98,312,665 bp
  • A to T, chromosome 15 at 98,482,111 bp
  • A to G, chromosome 16 at 4,648,296 bp
  • A to G, chromosome 16 at 9,663,518 bp
  • T to C, chromosome 16 at 14,224,003 bp
  • A to T, chromosome 16 at 76,292,061 bp
  • T to C, chromosome 16 at 91,037,415 bp
  • T to C, chromosome 17 at 17,387,542 bp
  • T to C, chromosome 18 at 36,032,375 bp
  • T to C, chromosome 18 at 60,526,271 bp
  • T to A, chromosome 18 at 62,933,903 bp
  • G to T, chromosome 19 at 4,161,196 bp
  • C to T, chromosome 19 at 5,665,384 bp
  • A to T, chromosome 19 at 9,005,453 bp
  • T to A, chromosome 19 at 11,110,343 bp
  • T to A, chromosome Y at 936,929 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8261 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067686-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.