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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR8272Btlr/Mmmh
Stock Number:
067695-MU
Citation ID:
RRID:MMRRC_067695-MU
Other Names:
R8272 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Slc12a1
Name: solute carrier family 12, member 1
Synonyms: Nkcc2, mBSC1, D630042G03Rik, urehr3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20495
Homologene: 286

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Casp8
Name: caspase 8
Synonyms: Mch5, MACH, FLICE, Caspase-8
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12370
HGNC: HGNC:1509
Homologene: 7657

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Card11
Name: caspase recruitment domain family, member 11
Synonyms: CARMA1, BIMP3, 2410011D02Rik, 0610008L17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 108723
Homologene: 13024

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Zdbf2
Name: zinc finger, DBF-type containing 2
Synonyms: 9330107J05Rik, 4930431J08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73884
Homologene: 52868

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Tmprss12
Name: transmembrane (C-terminal) protease, serine 12
Synonyms: 4930478A21Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 75002
VEGA: 15
Homologene: 16598

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
Gene Cloud
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Chuk
Name: conserved helix-loop-helix ubiquitous kinase
Synonyms: Chuk1, IKK1, IKK[a], IKK-alpha, IkappaB kinase alpha, IKK 1, IKK alpha, IKKalpha, IKK-1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12675
HGNC: HGNC:1974
Homologene: 979

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Ddx50
Name: DExD box helicase 50
Synonyms: GU2, RH-II/Gubeta, 4933429B04Rik, 8430408E17Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 94213
VEGA: 10
Homologene: 56986

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 58,833,742 bp
  • A to G, chromosome 1 at 63,305,983 bp
  • G to T, chromosome 1 at 85,931,397 bp
  • A to G, chromosome 1 at 118,332,404 bp
  • T to C, chromosome 1 at 162,639,426 bp
  • G to A, chromosome 1 at 172,527,839 bp
  • A to G, chromosome 2 at 27,461,713 bp
  • G to A, chromosome 2 at 104,026,154 bp
  • T to A, chromosome 2 at 117,262,854 bp
  • T to C, chromosome 2 at 125,228,816 bp
  • G to T, chromosome 2 at 140,660,697 bp
  • A to T, chromosome 2 at 155,632,904 bp
  • T to G, chromosome 3 at 5,243,867 bp
  • T to C, chromosome 3 at 24,058,202 bp
  • C to T, chromosome 3 at 95,125,860 bp
  • G to A, chromosome 4 at 43,984,064 bp
  • T to A, chromosome 4 at 53,854,332 bp
  • A to G, chromosome 4 at 96,723,946 bp
  • A to G, chromosome 4 at 156,350,078 bp
  • T to C, chromosome 5 at 69,110,460 bp
  • A to G, chromosome 5 at 140,890,039 bp
  • G to A, chromosome 6 at 8,154,175 bp
  • A to T, chromosome 6 at 27,363,282 bp
  • T to C, chromosome 6 at 28,662,193 bp
  • A to G, chromosome 6 at 42,801,104 bp
  • A to T, chromosome 6 at 48,448,519 bp
  • T to C, chromosome 6 at 120,900,215 bp
  • T to A, chromosome 6 at 122,711,777 bp
  • A to G, chromosome 7 at 6,373,214 bp
  • A to G, chromosome 7 at 45,228,742 bp
  • A to T, chromosome 7 at 84,947,609 bp
  • A to G, chromosome 7 at 108,925,896 bp
  • A to G, chromosome 7 at 120,407,442 bp
  • A to G, chromosome 8 at 25,586,656 bp
  • G to T, chromosome 8 at 71,989,091 bp
  • C to A, chromosome 9 at 20,638,864 bp
  • T to C, chromosome 9 at 40,304,524 bp
  • A to G, chromosome 9 at 56,258,898 bp
  • A to C, chromosome 9 at 109,559,760 bp
  • C to T, chromosome 10 at 26,303,668 bp
  • A to G, chromosome 10 at 62,621,477 bp
  • T to G, chromosome 10 at 65,002,598 bp
  • T to A, chromosome 10 at 85,114,567 bp
  • T to A, chromosome 11 at 6,229,327 bp
  • C to A, chromosome 11 at 60,851,754 bp
  • G to A, chromosome 12 at 98,688,530 bp
  • T to A, chromosome 12 at 118,111,017 bp
  • G to A, chromosome 13 at 21,180,610 bp
  • T to G, chromosome 13 at 24,036,579 bp
  • A to T, chromosome 13 at 32,845,737 bp
  • G to T, chromosome 13 at 41,816,154 bp
  • A to T, chromosome 13 at 113,368,355 bp
  • G to T, chromosome 14 at 99,302,104 bp
  • G to T, chromosome 15 at 4,516,736 bp
  • A to T, chromosome 15 at 100,282,265 bp
  • T to C, chromosome 16 at 14,218,854 bp
  • T to C, chromosome 16 at 17,209,105 bp
  • A to G, chromosome 17 at 20,596,228 bp
  • A to G, chromosome 17 at 32,057,189 bp
  • G to T, chromosome 17 at 34,356,301 bp
  • G to T, chromosome 17 at 50,047,380 bp
  • T to A, chromosome 18 at 37,146,189 bp
  • A to T, chromosome 19 at 4,601,730 bp
  • G to A, chromosome 19 at 6,047,941 bp
  • G to A, chromosome 19 at 13,063,431 bp
  • A to G, chromosome 19 at 16,749,845 bp
  • A to G, chromosome 19 at 44,103,736 bp
  • A to T, chromosome X at 170,672,725 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8272 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
067695-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.