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Strain Name:
C57BL/6J-MtgxR8276Btlr/Mmmh
Stock Number:
067699-MU
Citation ID:
RRID:MMRRC_067699-MU
Other Names:
R8276 (G1)
Major Collection:

Strain Information

Tnrc6b
Name: trinucleotide repeat containing 6b
Synonyms: A730065C02Rik, D230019K20Rik, 2700090M07Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 213988
VEGA: 15
Homologene: 66194
Zmym1
Name: zinc finger, MYM domain containing 1
Synonyms: 5830412B09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68310
Homologene: 32904
Gng5
Name: G protein subunit gamma 5
Synonyms: G(y)5
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14707
Homologene: 3861
Shroom3
Name: shroom family member 3
Synonyms: D5Ertd287e, Shrm, Shrm3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 27428
Homologene: 9263
Polr2a
Name: polymerase (RNA) II (DNA directed) polypeptide A
Synonyms: 220kDa, Rpo2-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20020
HGNC: HGNC:9187
Homologene: 721
Mib1
Name: MIB E3 ubiquitin protein ligase 1
Synonyms: E430019M12Rik, Mib, skeletrophin, mind bomb-1, mindbomb
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225164
Homologene: 10810
Rrm1
Name: ribonucleotide reductase M1
Synonyms: RnrM1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20133
Homologene: 806
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 80,528,281 bp
  • T to C, chromosome 1 at 92,886,809 bp
  • A to T, chromosome 1 at 183,436,419 bp
  • A to G, chromosome 2 at 23,069,551 bp
  • T to C, chromosome 2 at 80,349,270 bp
  • T to C, chromosome 2 at 112,640,617 bp
  • A to G, chromosome 3 at 32,482,423 bp
  • A to G, chromosome 3 at 82,864,588 bp
  • A to C, chromosome 3 at 135,371,897 bp
  • A to G, chromosome 3 at 146,500,503 bp
  • A to G, chromosome 4 at 63,132,956 bp
  • A to T, chromosome 4 at 127,054,258 bp
  • C to T, chromosome 4 at 131,779,173 bp
  • A to T, chromosome 5 at 39,614,803 bp
  • C to A, chromosome 5 at 86,142,650 bp
  • A to T, chromosome 5 at 92,940,480 bp
  • T to C, chromosome 5 at 112,795,407 bp
  • G to T, chromosome 6 at 148,173,901 bp
  • G to A, chromosome 7 at 5,317,495 bp
  • A to T, chromosome 7 at 16,079,084 bp
  • T to A, chromosome 7 at 64,372,486 bp
  • A to T, chromosome 7 at 65,343,796 bp
  • G to A, chromosome 7 at 66,079,804 bp
  • G to A, chromosome 7 at 102,460,852 bp
  • A to T, chromosome 7 at 104,553,315 bp
  • A to G, chromosome 8 at 88,734,928 bp
  • A to C, chromosome 8 at 109,670,721 bp
  • T to C, chromosome 9 at 86,517,039 bp
  • A to T, chromosome 10 at 94,582,308 bp
  • G to A, chromosome 11 at 69,748,056 bp
  • T to A, chromosome 11 at 79,527,490 bp
  • A to G, chromosome 12 at 103,001,863 bp
  • G to T, chromosome 12 at 103,947,169 bp
  • T to A, chromosome 14 at 32,663,928 bp
  • T to A, chromosome 14 at 50,710,830 bp
  • A to T, chromosome 14 at 53,717,238 bp
  • T to A, chromosome 15 at 80,880,717 bp
  • C to G, chromosome 15 at 81,650,028 bp
  • T to A, chromosome 15 at 85,882,731 bp
  • G to A, chromosome 17 at 18,305,387 bp
  • C to G, chromosome 17 at 24,163,600 bp
  • G to T, chromosome 17 at 24,642,260 bp
  • G to A, chromosome 17 at 78,791,539 bp
  • T to A, chromosome 18 at 4,674,318 bp
  • T to C, chromosome 18 at 10,751,880 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8276 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067699-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.