Strain Name:
C57BL/6J-MtgxR8276Btlr/Mmmh
Stock Number:
067699-MU
Citation ID:
RRID:MMRRC_067699-MU
Other Names:
R8276 (G1)
Major Collection:

Strain Information

Tnrc6b
Name: trinucleotide repeat containing 6b
Synonyms: A730065C02Rik, D230019K20Rik, 2700090M07Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 213988
VEGA: 15
Homologene: 66194
Zmym1
Name: zinc finger, MYM domain containing 1
Synonyms: 5830412B09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68310
Homologene: 32904
Gng5
Name: G protein subunit gamma 5
Synonyms: G(y)5
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14707
HGNC: HGNC:4408
Homologene: 3861
Shroom3
Name: shroom family member 3
Synonyms: D5Ertd287e, Shrm, Shrm3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 27428
Homologene: 9263
Polr2a
Name: polymerase (RNA) II (DNA directed) polypeptide A
Synonyms: 220kDa, Rpo2-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20020
HGNC: HGNC:9187
Homologene: 721
Mib1
Name: MIB E3 ubiquitin protein ligase 1
Synonyms: Mib, E430019M12Rik, skeletrophin, mind bomb-1, mindbomb
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225164
Homologene: 10810
Rrm1
Name: ribonucleotide reductase M1
Synonyms: RnrM1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20133
Homologene: 806
Dop1a
Name: DOP1 leucine zipper like protein A
Synonyms: B130005I07Rik, D9Ertd809e, Dopey1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 320615
Homologene: 26645
Ep300
Name: E1A binding protein p300
Synonyms: p300, KAT3B
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 328572
HGNC: HGNC:3373
Homologene: 1094
Uba6
Name: ubiquitin-like modifier activating enzyme 6
Synonyms: 5730469D23Rik, Ube1l2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231380
Homologene: 10080
Tjp1
Name: tight junction protein 1
Synonyms: ZO-1, ZO1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 21872
Homologene: 2445
Dnajc10
Name: DnaJ heat shock protein family (Hsp40) member C10
Synonyms: JPDI, 1200006L06Rik, D2Ertd706e, ERdj5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66861
Homologene: 10358
Fan1
Name: FANCD2/FANCI-associated nuclease 1
Synonyms: 6030441H18Rik, Mtmr15
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330554
Homologene: 45598
Hhipl2
Name: hedgehog interacting protein-like 2
Synonyms: 4930507C10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 78772
Homologene: 11690
Acbd5
Name: acyl-Coenzyme A binding domain containing 5
Synonyms: 1300014E15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74159
Homologene: 12541
Heatr5b
Name: HEAT repeat containing 5B
Synonyms: D330050P16Rik, 2010013B10Rik, A230048G03Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 320473
VEGA: 17
Homologene: 25536
Or52h9
Name: olfactory receptor family 52 subfamily H member 9
Synonyms: GA_x6K02T2PBJ9-7179540-7180481, MOR31-11, Olfr651
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258809
Homologene: 64922
Pkd1l3
Name: polycystic kidney disease 1 like 3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244646
Homologene: 14627
Dock10
Name: dedicator of cytokinesis 10
Synonyms: Jr5, Jr4, ZIZ3, 9330153B10Rik, A630054M16Rik, Zizimin3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 210293
Homologene: 45952
Ryr3
Name: ryanodine receptor 3
Synonyms: calcium release channel isoform 3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20192
Homologene: 68151
Nlrp2
Name: NLR family, pyrin domain containing 2
Synonyms: PYPAF2, E330007A02Rik, Nalp2, Pan1, Nbs1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232827
Homologene: 56789
Unc79
Name: unc-79 homolog
Synonyms: 9030205A07Rik, Mlca3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217843
Homologene: 41397
Rbm46
Name: RNA binding motif protein 46
Synonyms: LOC329687, ENSMUSG00000033882
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 633285
Homologene: 17016
Vmn2r93
Name: vomeronasal 2, receptor 93
Synonyms: EG627132
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 627132
Homologene: 129750
Trav7-6
Name: T cell receptor alpha variable 7-6
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 213480
Zfp541
Name: zinc finger protein 541
Synonyms: EG666528
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 666528
Homologene: 12991
Cyld
Name: CYLD lysine 63 deubiquitinase
Synonyms: CYLD1, 2010013M14Rik, C130039D01Rik, 2900009M21Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 74256
HGNC: HGNC:2584
Homologene: 9069
Tmcc3
Name: transmembrane and coiled coil domains 3
Synonyms: C630016B22Rik, LOC380656, A230066D03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 319880
Homologene: 10792
Slc9b1
Name: solute carrier family 9, subfamily B (NHA1, cation proton antiporter 1), member 1
Synonyms: 4933424B12Rik, 1700094G20Rik, 4933425K02Rik, Nhedc1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74446
Homologene: 49914
3425401B19Rik
Name: RIKEN cDNA 3425401B19 gene
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100504518
VEGA: 14
Homologene: 54908
Myo18b
Name: myosin XVIIIb
Synonyms: 4932408L24Rik, 4933411E19Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 74376
Homologene: 53435
Zfp618
Name: zinc finger protein 618
Synonyms: D430033D05Rik, 2810040O04Rik, 2810031P15Rik, Nedd10
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 72701
Homologene: 18975
Ptpru
Name: protein tyrosine phosphatase receptor type U
Synonyms: Ptprl, RPTPlambda
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19273
HGNC: HGNC:9683
Homologene: 4168
Ankmy1
Name: ankyrin repeat and MYND domain containing 1
Synonyms: 4930483I10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241158
Homologene: 9561
Far2
Name: fatty acyl CoA reductase 2
Synonyms: Mlstd1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330450
Homologene: 69247
Serpina1e
Name: serine (or cysteine) peptidase inhibitor, clade A, member 1E
Synonyms: PI5, Spi1-5
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20704
HGNC: HGNC:8941
Homologene: 20103
Or11h23
Name: olfactory receptor family 11 subfamily G member 23
Synonyms: GA_x6K02T2PMLR-6454789-6455712, MOR106-9P, Olfr748
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 258113
Homologene: 134081
Trmu
Name: tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase
Synonyms: 1600025P05Rik, 1110005N20Rik, Trmt1, Mtu1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 72026
Homologene: 5589
Amdhd2
Name: amidohydrolase domain containing 2
Synonyms: 5730457F11Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 245847
Homologene: 9311
Hs3st1
Name: heparan sulfate (glucosamine) 3-O-sulfotransferase 1
Synonyms: 3-OST, D5Wsu110e
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 15476
HGNC: HGNC:5194
Homologene: 3751
Evi2a
Name: ecotropic viral integration site 2a
Synonyms: Evi-2, Evi2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14017
HGNC: HGNC:3499
Homologene: 49234
Nherf2
Name: NHERF family PDZ scaffold protein 2
Synonyms: Nherf2, Octs2, Tka-1, Sip-1, E3karp, 1200011K07Rik, Sip1, 0610011L07Rik, 2010007A20Rik, Sryip1, Slc9a3r2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 65962
VEGA: 17
Homologene: 56962
Kcnmb3
Name: potassium large conductance calcium-activated channel, subfamily M, beta member 3
Synonyms: Gm5707
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 100502876
HGNC: HGNC:6287
Homologene: 18141
9430020K01Rik
Name:
Type: Gene
Species: Mouse
Chromosome: 18
Vimp
Name:
Type: Gene
Species: Mouse
Chromosome: 7
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 80,528,281 bp
  • T to C, chromosome 1 at 92,886,809 bp
  • A to T, chromosome 1 at 183,436,419 bp
  • A to G, chromosome 2 at 23,069,551 bp
  • T to C, chromosome 2 at 80,349,270 bp
  • T to C, chromosome 2 at 112,640,617 bp
  • A to G, chromosome 3 at 32,482,423 bp
  • A to G, chromosome 3 at 82,864,588 bp
  • A to C, chromosome 3 at 135,371,897 bp
  • A to G, chromosome 3 at 146,500,503 bp
  • A to G, chromosome 4 at 63,132,956 bp
  • A to T, chromosome 4 at 127,054,258 bp
  • C to T, chromosome 4 at 131,779,173 bp
  • A to T, chromosome 5 at 39,614,803 bp
  • C to A, chromosome 5 at 86,142,650 bp
  • A to T, chromosome 5 at 92,940,480 bp
  • T to C, chromosome 5 at 112,795,407 bp
  • G to T, chromosome 6 at 148,173,901 bp
  • G to A, chromosome 7 at 5,317,495 bp
  • A to T, chromosome 7 at 16,079,084 bp
  • T to A, chromosome 7 at 64,372,486 bp
  • A to T, chromosome 7 at 65,343,796 bp
  • G to A, chromosome 7 at 66,079,804 bp
  • G to A, chromosome 7 at 102,460,852 bp
  • A to T, chromosome 7 at 104,553,315 bp
  • A to G, chromosome 8 at 88,734,928 bp
  • A to C, chromosome 8 at 109,670,721 bp
  • T to C, chromosome 9 at 86,517,039 bp
  • A to T, chromosome 10 at 94,582,308 bp
  • G to A, chromosome 11 at 69,748,056 bp
  • T to A, chromosome 11 at 79,527,490 bp
  • A to G, chromosome 12 at 103,001,863 bp
  • G to T, chromosome 12 at 103,947,169 bp
  • T to A, chromosome 14 at 32,663,928 bp
  • T to A, chromosome 14 at 50,710,830 bp
  • A to T, chromosome 14 at 53,717,238 bp
  • T to A, chromosome 15 at 80,880,717 bp
  • C to G, chromosome 15 at 81,650,028 bp
  • T to A, chromosome 15 at 85,882,731 bp
  • G to A, chromosome 17 at 18,305,387 bp
  • C to G, chromosome 17 at 24,163,600 bp
  • G to T, chromosome 17 at 24,642,260 bp
  • G to A, chromosome 17 at 78,791,539 bp
  • T to A, chromosome 18 at 4,674,318 bp
  • T to C, chromosome 18 at 10,751,880 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8276 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067699-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.