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Strain Name:
C57BL/6J-MtgxR8307Btlr/Mmmh
Stock Number:
067717-MU
Citation ID:
RRID:MMRRC_067717-MU
Other Names:
R8307 (G1)
Major Collection:

Strain Information

Pln
Name: phospholamban
Synonyms: PLB
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18821
VEGA: 10
HGNC: HGNC:9080
Homologene: 2002
Hmmr
Name: hyaluronan mediated motility receptor (RHAMM)
Synonyms: CD168, Rhamm
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15366
HGNC: HGNC:5012
Homologene: 8271
Fancl
Name: Fanconi anemia, complementation group L
Synonyms: 2010322C19Rik, gcd, Pog, Phf9, B230118H11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67030
Homologene: 9987
Atp2c1
Name: ATPase, Ca++-sequestering
Synonyms: SPCA, ATP2C1A, PMR1, 1700121J11Rik, D930003G21Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235574
Homologene: 56672
Csde1
Name: cold shock domain containing E1, RNA binding
Synonyms: unr, D3Jfr1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229663
Homologene: 5179
Tasor
Name: transcription activation suppressor
Synonyms: 4933409E02Rik, D14Abb1e, MommeD6, Fam208a
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218850
VEGA: 14
Homologene: 9062
Dennd4c
Name: DENN domain containing 4C
Synonyms: 1700065A05Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 329877
Homologene: 23057
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 65,245,735 bp
  • A to G, chromosome 2 at 31,396,115 bp
  • A to G, chromosome 2 at 36,422,321 bp
  • G to A, chromosome 2 at 88,831,289 bp
  • C to A, chromosome 2 at 125,505,482 bp
  • A to G, chromosome 3 at 89,243,916 bp
  • A to G, chromosome 3 at 103,039,073 bp
  • A to C, chromosome 4 at 86,825,872 bp
  • G to A, chromosome 4 at 98,778,678 bp
  • A to T, chromosome 4 at 138,317,962 bp
  • C to A, chromosome 4 at 147,941,380 bp
  • A to G, chromosome 5 at 36,947,086 bp
  • T to C, chromosome 5 at 41,821,155 bp
  • T to G, chromosome 5 at 94,683,557 bp
  • T to A, chromosome 5 at 110,128,197 bp
  • A to G, chromosome 5 at 147,306,667 bp
  • A to G, chromosome 6 at 67,766,067 bp
  • A to G, chromosome 6 at 83,379,927 bp
  • A to G, chromosome 7 at 11,307,132 bp
  • G to A, chromosome 7 at 44,957,662 bp
  • A to G, chromosome 7 at 85,007,062 bp
  • T to A, chromosome 7 at 127,525,369 bp
  • A to G, chromosome 7 at 142,174,849 bp
  • C to T, chromosome 8 at 123,206,241 bp
  • G to A, chromosome 9 at 24,503,001 bp
  • A to G, chromosome 9 at 31,859,011 bp
  • G to A, chromosome 9 at 65,279,004 bp
  • A to G, chromosome 9 at 96,839,084 bp
  • C to T, chromosome 9 at 105,442,831 bp
  • T to C, chromosome 10 at 53,343,879 bp
  • A to G, chromosome 10 at 61,726,392 bp
  • T to A, chromosome 10 at 74,506,475 bp
  • T to C, chromosome 10 at 129,174,232 bp
  • T to A, chromosome 11 at 9,277,922 bp
  • A to G, chromosome 11 at 26,399,642 bp
  • A to T, chromosome 11 at 34,310,362 bp
  • G to A, chromosome 11 at 40,721,672 bp
  • G to A, chromosome 11 at 58,151,594 bp
  • G to A, chromosome 11 at 58,647,083 bp
  • A to T, chromosome 11 at 79,690,774 bp
  • A to T, chromosome 11 at 101,188,876 bp
  • A to T, chromosome 11 at 116,659,373 bp
  • G to A, chromosome 11 at 117,143,416 bp
  • C to A, chromosome 12 at 55,741,523 bp
  • T to C, chromosome 13 at 97,194,199 bp
  • G to T, chromosome 13 at 112,487,747 bp
  • G to T, chromosome 14 at 27,471,665 bp
  • C to A, chromosome 15 at 21,358,863 bp
  • T to A, chromosome 15 at 21,358,864 bp
  • A to G, chromosome 15 at 99,423,177 bp
  • A to G, chromosome 15 at 101,559,199 bp
  • A to G, chromosome 16 at 4,497,145 bp
  • C to T, chromosome 16 at 17,858,378 bp
  • A to C, chromosome 16 at 21,495,902 bp
  • T to C, chromosome 16 at 73,956,610 bp
  • C to A, chromosome 17 at 20,583,057 bp
  • C to A, chromosome 17 at 56,626,573 bp
  • C to T, chromosome 18 at 20,575,064 bp
  • A to G, chromosome 18 at 37,807,794 bp
  • T to A, chromosome 18 at 78,022,679 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8307 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067717-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.