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Strain Name:
C57BL/6J-MtgxR8312Btlr/Mmmh
Stock Number:
067718-MU
Citation ID:
RRID:MMRRC_067718-MU
Other Names:
R8312 (G1)
Major Collection:

Strain Information

Hapln1
Name: hyaluronan and proteoglycan link protein 1
Synonyms: link protein, cartilage linking protein 1, Crtl1l, Crtl1, CLP, LP-1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 12950
VEGA: 13
HGNC: HGNC:2380
Homologene: 1420
Pax3
Name: paired box 3
Synonyms: Pax-3, Splchl2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18505
HGNC: HGNC:8617
Homologene: 22494
Pkd1
Name: polycystin 1, transient receptor potential channel interacting
Synonyms: polycystin-1, PC1, PC-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18763
VEGA: 17
HGNC: HGNC:9008
Homologene: 250
Itga6
Name: integrin alpha 6
Synonyms: Cd49f, 5033401O05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16403
HGNC: HGNC:6142
Homologene: 20091
Htr4
Name: 5 hydroxytryptamine (serotonin) receptor 4
Synonyms: 5-HT4L, 5-HT4
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 15562
VEGA: 18
HGNC: HGNC:5299
Homologene: 20243
Gli2
Name: GLI-Kruppel family member GLI2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14633
HGNC: HGNC:4318
Homologene: 12725
Zmym6
Name: zinc finger, MYM-type 6
Synonyms: D4Wsu24e, 9330177P20Rik, Zfp258
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100177
Homologene: 5186
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 78,195,369 bp
  • G to T, chromosome 1 at 90,813,690 bp
  • A to G, chromosome 1 at 106,747,486 bp
  • A to T, chromosome 1 at 118,868,112 bp
  • A to G, chromosome 1 at 150,738,764 bp
  • G to A, chromosome 1 at 174,240,211 bp
  • A to T, chromosome 2 at 26,357,789 bp
  • A to T, chromosome 2 at 71,855,953 bp
  • G to A, chromosome 2 at 76,951,648 bp
  • C to A, chromosome 2 at 164,238,171 bp
  • A to T, chromosome 2 at 181,596,875 bp
  • C to G, chromosome 3 at 87,800,484 bp
  • G to C, chromosome 3 at 92,850,459 bp
  • C to A, chromosome 3 at 108,080,116 bp
  • C to T, chromosome 3 at 108,386,357 bp
  • A to G, chromosome 3 at 142,599,051 bp
  • C to A, chromosome 3 at 142,599,054 bp
  • A to T, chromosome 4 at 43,854,461 bp
  • A to T, chromosome 4 at 49,449,142 bp
  • A to G, chromosome 4 at 116,430,486 bp
  • A to G, chromosome 4 at 116,699,201 bp
  • A to G, chromosome 4 at 127,123,834 bp
  • A to T, chromosome 4 at 129,839,634 bp
  • T to A, chromosome 4 at 130,054,451 bp
  • T to A, chromosome 4 at 138,223,439 bp
  • T to C, chromosome 5 at 4,894,141 bp
  • T to C, chromosome 5 at 24,134,967 bp
  • A to G, chromosome 5 at 30,894,506 bp
  • C to T, chromosome 5 at 32,328,485 bp
  • C to T, chromosome 5 at 76,636,899 bp
  • T to C, chromosome 5 at 87,242,940 bp
  • T to A, chromosome 5 at 96,588,191 bp
  • C to A, chromosome 5 at 115,150,207 bp
  • GC to GCTCC, chromosome 6 at 4,756,452 bp
  • T to C, chromosome 6 at 12,471,182 bp
  • T to A, chromosome 6 at 35,023,141 bp
  • A to G, chromosome 6 at 66,804,857 bp
  • T to A, chromosome 6 at 86,457,831 bp
  • C to T, chromosome 7 at 12,991,287 bp
  • T to C, chromosome 7 at 30,911,469 bp
  • G to A, chromosome 7 at 55,933,302 bp
  • A to G, chromosome 7 at 132,876,600 bp
  • C to T, chromosome 8 at 25,663,252 bp
  • A to G, chromosome 8 at 85,274,869 bp
  • T to A, chromosome 8 at 123,269,810 bp
  • G to T, chromosome 8 at 125,762,850 bp
  • T to C, chromosome 9 at 21,115,649 bp
  • A to T, chromosome 9 at 37,646,297 bp
  • G to A, chromosome 9 at 87,169,659 bp
  • A to G, chromosome 9 at 108,644,274 bp
  • T to C, chromosome 10 at 59,438,810 bp
  • T to C, chromosome 10 at 114,413,287 bp
  • A to G, chromosome 10 at 128,990,478 bp
  • G to A, chromosome 11 at 69,988,701 bp
  • G to A, chromosome 11 at 72,499,708 bp
  • A to C, chromosome 11 at 102,436,247 bp
  • T to A, chromosome 12 at 73,760,584 bp
  • T to C, chromosome 12 at 84,615,416 bp
  • C to A, chromosome 13 at 42,155,177 bp
  • T to G, chromosome 13 at 51,442,718 bp
  • C to T, chromosome 13 at 89,601,444 bp
  • T to C, chromosome 16 at 19,407,237 bp
  • C to T, chromosome 16 at 33,726,675 bp
  • G to T, chromosome 17 at 6,207,058 bp
  • A to G, chromosome 17 at 24,567,128 bp
  • T to C, chromosome 17 at 34,858,145 bp
  • A to G, chromosome 18 at 10,511,587 bp
  • A to G, chromosome 18 at 35,660,808 bp
  • C to G, chromosome 18 at 62,437,478 bp
  • A to G, chromosome 18 at 74,733,962 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8312 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067718-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.