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Strain Name:
C57BL/6J-MtgxR8314Btlr/Mmmh
Stock Number:
067719-MU
Citation ID:
RRID:MMRRC_067719-MU
Other Names:
R8314 (G1)
Major Collection:

Strain Information

Tnpo1
Name: transportin 1
Synonyms: D13Ertd688e, Kpnb2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 238799
HGNC: HGNC:6401
Homologene: 5358
Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779
Ak4
Name: adenylate kinase 4
Synonyms: Ak-4, D4Ertd274e, Ak-3, Ak3l1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11639
Homologene: 100316
Fbxl3
Name: F-box and leucine-rich repeat protein 3
Synonyms: Fbl3a, Fbxl3a, Ovtm, Play68
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 50789
Homologene: 8127
Pip5k1b
Name: phosphatidylinositol-4-phosphate 5-kinase, type 1 beta
Synonyms: Pipk5b
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18719
HGNC: HGNC:8995
Homologene: 100644
Rabep1
Name: rabaptin, RAB GTPase binding effector protein 1
Synonyms: RAB5 effector protein, neurocrescin, rabaptin-5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 54189
Homologene: 3451
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 24,021,921 bp
  • G to A, chromosome 1 at 43,934,227 bp
  • A to T, chromosome 1 at 53,346,655 bp
  • A to G, chromosome 1 at 171,384,727 bp
  • T to A, chromosome 2 at 15,752,832 bp
  • A to G, chromosome 2 at 25,557,965 bp
  • T to A, chromosome 2 at 35,175,143 bp
  • A to G, chromosome 2 at 87,711,202 bp
  • T to C, chromosome 2 at 90,052,097 bp
  • A to G, chromosome 2 at 125,655,616 bp
  • A to G, chromosome 2 at 144,502,958 bp
  • T to C, chromosome 3 at 56,009,251 bp
  • T to A, chromosome 3 at 88,621,293 bp
  • T to C, chromosome 3 at 89,188,146 bp
  • C to T, chromosome 4 at 101,463,585 bp
  • A to C, chromosome 4 at 115,458,338 bp
  • C to A, chromosome 4 at 137,539,675 bp
  • T to A, chromosome 4 at 156,234,013 bp
  • A to G, chromosome 5 at 53,897,047 bp
  • T to A, chromosome 5 at 110,755,753 bp
  • A to C, chromosome 6 at 122,320,978 bp
  • C to A, chromosome 7 at 19,245,015 bp
  • T to C, chromosome 7 at 24,750,588 bp
  • C to T, chromosome 7 at 30,578,922 bp
  • T to C, chromosome 7 at 33,173,157 bp
  • T to C, chromosome 7 at 103,612,047 bp
  • T to C, chromosome 7 at 143,695,096 bp
  • A to T, chromosome 8 at 16,158,244 bp
  • C to T, chromosome 8 at 83,938,389 bp
  • T to C, chromosome 8 at 99,171,379 bp
  • A to G, chromosome 8 at 106,168,958 bp
  • G to A, chromosome 9 at 7,272,931 bp
  • T to C, chromosome 9 at 7,872,941 bp
  • A to G, chromosome 9 at 39,319,305 bp
  • T to A, chromosome 9 at 122,948,928 bp
  • T to A, chromosome 10 at 24,252,540 bp
  • A to G, chromosome 10 at 70,875,276 bp
  • G to A, chromosome 10 at 79,481,479 bp
  • A to T, chromosome 10 at 80,723,539 bp
  • A to G, chromosome 10 at 127,295,721 bp
  • T to C, chromosome 11 at 68,385,624 bp
  • T to C, chromosome 11 at 70,316,172 bp
  • A to G, chromosome 11 at 70,893,660 bp
  • T to G, chromosome 11 at 73,799,742 bp
  • G to A, chromosome 11 at 102,913,074 bp
  • G to T, chromosome 12 at 30,884,851 bp
  • A to G, chromosome 12 at 81,379,135 bp
  • A to T, chromosome 12 at 111,823,281 bp
  • T to C, chromosome 13 at 3,912,672 bp
  • G to A, chromosome 13 at 30,708,931 bp
  • G to T, chromosome 13 at 41,272,634 bp
  • A to G, chromosome 13 at 64,184,751 bp
  • T to C, chromosome 13 at 98,884,625 bp
  • A to G, chromosome 14 at 33,622,547 bp
  • A to T, chromosome 14 at 46,769,873 bp
  • G to A, chromosome 14 at 54,253,812 bp
  • T to C, chromosome 14 at 65,029,030 bp
  • C to T, chromosome 14 at 103,089,440 bp
  • A to G, chromosome 15 at 5,178,873 bp
  • C to A, chromosome 15 at 76,710,180 bp
  • A to G, chromosome 15 at 97,010,309 bp
  • T to C, chromosome 15 at 98,290,583 bp
  • A to T, chromosome 16 at 10,510,988 bp
  • T to C, chromosome 16 at 96,315,449 bp
  • A to T, chromosome 17 at 24,901,774 bp
  • G to T, chromosome 17 at 30,984,832 bp
  • C to A, chromosome 17 at 33,067,064 bp
  • G to A, chromosome 18 at 11,720,625 bp
  • T to A, chromosome 19 at 24,355,199 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8314 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067719-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.