Strain Name:
C57BL/6J-MtgxR8320Btlr/Mmmh
Stock Number:
067722-MU
Citation ID:
RRID:MMRRC_067722-MU
Other Names:
R8320 (G1)
Major Collection:

Strain Information

Smarca4
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Synonyms: SNF2beta, SW1/SNF, Brg1, b2b692Clo, b2b508.1Clo
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20586
Homologene: 135927
Septin4
Name: septin 4
Synonyms: Pnutl2, Bh5, Gm11492, septin H5, cell division control-related protein 2b, ARTS, Sept4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18952
HGNC: HGNC:9165
Homologene: 6107
Slc6a2
Name: solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2
Synonyms: NE transporter, NET, Slc6a5, norepinephrine transporter
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 20538
Homologene: 816
Foxj2
Name: forkhead box J2
Synonyms: Fhx
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 60611
Homologene: 10187
Ttc3
Name: tetratricopeptide repeat domain 3
Synonyms: D16Ium21, D16Ium21e, TPRD, 2610202A04Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 22129
Homologene: 2487
Rfc1
Name: replication factor C (activator 1) 1
Synonyms: Alp145, Recc1, 140kDa, RFC140
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19687
HGNC: HGNC:9969
Homologene: 2187
Ercc6
Name: excision repair cross-complementing rodent repair deficiency, complementation group 6
Synonyms: C130058G22Rik, CS group B correcting gene, CSB
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 319955
VEGA: 14
HGNC: HGNC:3438
Homologene: 133552
Cobl
Name: cordon-bleu WH2 repeat
Synonyms: C530045F18Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12808
Homologene: 9058
Cnn3
Name: calponin 3, acidic
Synonyms: Calpo3, 1600014M03Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71994
HGNC: HGNC:2157
Homologene: 37533
Polr1a
Name: polymerase (RNA) I polypeptide A
Synonyms: RPA194, Rpo1-4, mRPA1, 194kDa, 2900087K15Rik, 3010014K16Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20019
Homologene: 7033
Stx7
Name: syntaxin 7
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 53331
VEGA: 10
Homologene: 37823
Rnf17
Name: ring finger protein 17
Synonyms: MMIP-2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 30054
VEGA: 14
Homologene: 23727
Dmxl2
Name: Dmx-like 2
Synonyms: 6430411K14Rik, E130119P06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235380
HGNC: HGNC:2938
Homologene: 41022
Tubb3
Name: tubulin, beta 3 class III
Synonyms: 3200002H15Rik, betaIII-tubulin, Tuj1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22152
Homologene: 68503
Erap1
Name: endoplasmic reticulum aminopeptidase 1
Synonyms: Arts1, PILSAP, ERAAP
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 80898
VEGA: 13
Homologene: 56754
Rasal1
Name: RAS protein activator like 1 (GAP1 like)
Synonyms: MRASAL
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19415
HGNC: HGNC:9873
Homologene: 3423
Egfr
Name: epidermal growth factor receptor
Synonyms: 9030024J15Rik, Wa5, Erbb, Errb1, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13649
HGNC: HGNC:3236
Homologene: 74545
Clec3a
Name: C-type lectin domain family 3, member a
Synonyms: 1110019O10Rik, Clecsf1, LOC330844
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 403395
HGNC: HGNC:2052
Homologene: 4205
Taf4b
Name: TATA-box binding protein associated factor 4b
Synonyms: 105kDa, 4932409F03Rik, Taf2c2, TAFII105, 2610524B04Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 72504
VEGA: 18
Homologene: 28266
Tpcn2
Name: two pore segment channel 2
Synonyms: D830047E22Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233979
Homologene: 16375
Bhlhe40
Name: basic helix-loop-helix family, member e40
Synonyms: cytokine response gene 8, Clast5, Stra13, Bhlhb2, C130042M06Rik, eip1 (E47 interaction protein 1), Stra14, Sharp2, DEC1, CR8
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20893
HGNC: HGNC:1046
Homologene: 2722
Syne2
Name: spectrin repeat containing, nuclear envelope 2
Synonyms: D12Ertd777e, Cpfl8, dice, 6820443O06Rik, nesprin-2, syne-2, Nesp2g, diminished cone electroretinogram
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319565
Homologene: 56700
Pigg
Name: phosphatidylinositol glycan anchor biosynthesis, class G
Synonyms: Gpi7
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 433931
Homologene: 39421
Clspn
Name: claspin
Synonyms: C85083, E130314M08Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269582
Homologene: 11138
Trpm1
Name: transient receptor potential cation channel, subfamily M, member 1
Synonyms: 4732499L03Rik, melastatin, Mlsn1, LTRPC1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17364
HGNC: HGNC:7146
Homologene: 19940
Neb
Name: nebulin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Poln
Name: DNA polymerase N
Synonyms: POL4P
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 272158
Homologene: 131253
Syna
Name: syncytin a
Synonyms: Gm52, syncytin-A
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 214292
Homologene: 86751
Lgi4
Name: leucine-rich repeat LGI family, member 4
Synonyms: clp
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243914
Homologene: 16408
Fbln2
Name: fibulin 2
Synonyms: 5730577E14Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14115
HGNC: HGNC:3601
Homologene: 1514
Grid2
Name: glutamate receptor, ionotropic, delta 2
Synonyms: tpr, B230104L07Rik, GluRdelta2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14804
HGNC: HGNC:4576
Homologene: 74399
Kif26b
Name: kinesin family member 26B
Synonyms: N-11 kinesin, D230039L06Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269152
Homologene: 18623
Asb10
Name: ankyrin repeat and SOCS box-containing 10
Synonyms: Asb-10
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 117590
Homologene: 135975
Gzmd
Name: granzyme D
Synonyms: Ctla5, Ctla-5, CCP5
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 14941
Homologene: 133275
Sval3
Name: seminal vesicle antigen-like 3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 387564
Homologene: 133811
Cntnap5a
Name: contactin associated protein-like 5A
Synonyms: Caspr5-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 636808
Homologene: 43974
Fbxw13
Name: F-box and WD-40 domain protein 13
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 211305
Homologene: 110776
Klra10
Name: killer cell lectin-like receptor subfamily A, member 10
Synonyms: Ly49i2, Ly49J
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16628
Homologene: 110821
Klk1b1
Name: kallikrein 1-related peptidase b1
Synonyms: tissue kallikrein, Klk1, mGK-1, mK1, TK
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16623
Homologene: 68141
Zscan4d
Name: zinc finger and SCAN domain containing 4D
Synonyms: EG545913
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 545913
Homologene: 85986
Gnl1
Name: guanine nucleotide binding protein-like 1
Synonyms: Gna-rs1, Gnal1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14670
HGNC: HGNC:4413
Homologene: 3862
Sars2
Name: seryl-aminoacyl-tRNA synthetase 2
Synonyms: 2410015F05Rik, D7Ertd353e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71984
Homologene: 6073
Zfp189
Name: zinc finger protein 189
Synonyms: C430015I23Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230162
Homologene: 20737
Mucl3
Name: mucin like 3
Synonyms: Dpcr1, LOC268949
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 268949
Homologene: 133813
Igkv2-137
Name: immunoglobulin kappa chain variable 2-137
Synonyms: Gm16955
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 692187
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 116,446,736 bp
  • T to C, chromosome 1 at 178,884,076 bp
  • C to A, chromosome 2 at 52,296,331 bp
  • T to G, chromosome 3 at 121,449,986 bp
  • C to G, chromosome 4 at 49,530,180 bp
  • A to G, chromosome 4 at 126,563,950 bp
  • A to G, chromosome 5 at 24,537,628 bp
  • A to G, chromosome 5 at 34,149,827 bp
  • A to T, chromosome 5 at 65,303,036 bp
  • G to A, chromosome 5 at 108,347,851 bp
  • C to T, chromosome 5 at 120,666,355 bp
  • A to G, chromosome 5 at 134,559,720 bp
  • G to A, chromosome 6 at 41,972,524 bp
  • T to C, chromosome 6 at 63,256,933 bp
  • GA to GAA, chromosome 6 at 67,556,170 bp
  • T to A, chromosome 6 at 71,941,384 bp
  • C to T, chromosome 6 at 91,257,767 bp
  • TG to TGG, chromosome 6 at 108,664,857 bp
  • T to A, chromosome 6 at 122,833,690 bp
  • A to G, chromosome 6 at 130,269,248 bp
  • C to T, chromosome 7 at 11,066,015 bp
  • C to T, chromosome 7 at 28,746,868 bp
  • T to C, chromosome 7 at 31,068,941 bp
  • C to T, chromosome 7 at 43,970,343 bp
  • A to G, chromosome 7 at 64,268,793 bp
  • C to T, chromosome 7 at 145,266,622 bp
  • C to T, chromosome 8 at 92,992,848 bp
  • T to C, chromosome 8 at 114,425,629 bp
  • T to C, chromosome 8 at 123,420,855 bp
  • G to T, chromosome 9 at 21,677,502 bp
  • A to G, chromosome 9 at 54,383,759 bp
  • G to T, chromosome 9 at 109,183,066 bp
  • G to T, chromosome 10 at 24,179,148 bp
  • A to G, chromosome 11 at 12,267,001 bp
  • T to C, chromosome 11 at 16,891,251 bp
  • T to C, chromosome 11 at 87,589,734 bp
  • T to C, chromosome 12 at 76,103,830 bp
  • G to C, chromosome 13 at 74,666,549 bp
  • T to C, chromosome 14 at 32,521,015 bp
  • A to G, chromosome 14 at 56,129,733 bp
  • TG to T, chromosome 14 at 56,424,542 bp
  • C to A, chromosome 16 at 94,418,676 bp
  • T to C, chromosome 17 at 35,643,638 bp
  • A to G, chromosome 17 at 35,982,598 bp
  • T to C, chromosome 18 at 14,783,692 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8320 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067722-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.