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Strain Detail Sheet

Published:
Revised: 03/02/2024

Strain Name:
C57BL/6J-MtgxR8324Btlr/Mmmh
Stock Number:
067725-MU
Citation ID:
RRID:MMRRC_067725-MU
Other Names:
R8324 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Pax3
Name: paired box 3
Synonyms: Pax-3, Splchl2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18505
HGNC: HGNC:8617
Homologene: 22494

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
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Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Myh9
Name: myosin, heavy polypeptide 9, non-muscle
Synonyms: D0Jmb2, E030044M24Rik, NMHC II-A, Myhn-1, Myhn1, myosin IIA, Fltn
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17886
HGNC: HGNC:7579
Homologene: 129835

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Kcnmb4
Name: potassium large conductance calcium-activated channel, subfamily M, beta member 4
Synonyms: Slowpoke beta 4, 2900045G12Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 58802
HGNC: HGNC:6289
Homologene: 8721

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Rictor
Name: RPTOR independent companion of MTOR, complex 2
Synonyms: 6030405M08Rik, D530039E11Rik, 4921505C17Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78757
VEGA: 15
Homologene: 34317

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Chst5
Name: carbohydrate sulfotransferase 5
Synonyms: I-GlcNAc6ST, GST-4
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56773
Homologene: 56927

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Eef1e1
Name: eukaryotic translation elongation factor 1 epsilon 1
Synonyms: 1110003A02Rik, AIMP3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 66143
VEGA: 13
HGNC: HGNC:3212
Homologene: 3161

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Rxra
Name: retinoid X receptor alpha
Synonyms: RXRalpha1, RXR alpha 1, 9530071D11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20181
Homologene: 2220

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 78,193,789 bp
  • T to A, chromosome 1 at 123,854,172 bp
  • T to C, chromosome 2 at 27,741,183 bp
  • T to A, chromosome 2 at 37,400,726 bp
  • G to A, chromosome 2 at 103,783,181 bp
  • A to G, chromosome 2 at 119,123,831 bp
  • A to G, chromosome 2 at 120,727,325 bp
  • G to A, chromosome 2 at 157,779,815 bp
  • C to G, chromosome 3 at 114,164,410 bp
  • T to C, chromosome 3 at 142,829,010 bp
  • C to T, chromosome 4 at 56,772,491 bp
  • T to C, chromosome 4 at 88,803,638 bp
  • C to G, chromosome 4 at 137,518,979 bp
  • A to C, chromosome 4 at 144,395,857 bp
  • T to G, chromosome 5 at 45,695,668 bp
  • T to C, chromosome 5 at 97,938,509 bp
  • A to G, chromosome 6 at 34,102,571 bp
  • G to T, chromosome 6 at 48,597,345 bp
  • A to G, chromosome 6 at 80,021,991 bp
  • T to A, chromosome 6 at 83,329,974 bp
  • T to C, chromosome 6 at 121,337,414 bp
  • T to C, chromosome 6 at 146,328,398 bp
  • G to T, chromosome 7 at 27,653,969 bp
  • A to G, chromosome 7 at 79,091,056 bp
  • G to A, chromosome 7 at 104,618,114 bp
  • T to C, chromosome 7 at 107,590,363 bp
  • A to G, chromosome 8 at 19,123,798 bp
  • A to T, chromosome 8 at 111,890,508 bp
  • G to A, chromosome 8 at 114,489,005 bp
  • A to T, chromosome 9 at 7,276,636 bp
  • A to T, chromosome 9 at 56,207,476 bp
  • T to A, chromosome 9 at 105,755,654 bp
  • A to G, chromosome 10 at 62,507,665 bp
  • A to T, chromosome 10 at 79,378,893 bp
  • T to C, chromosome 10 at 80,897,371 bp
  • A to G, chromosome 10 at 116,418,314 bp
  • T to C, chromosome 11 at 9,290,395 bp
  • A to G, chromosome 11 at 16,858,971 bp
  • A to C, chromosome 11 at 16,908,885 bp
  • T to A, chromosome 11 at 52,022,879 bp
  • T to C, chromosome 11 at 72,445,288 bp
  • T to A, chromosome 11 at 80,557,521 bp
  • T to C, chromosome 12 at 76,619,162 bp
  • T to C, chromosome 12 at 83,786,619 bp
  • C to T, chromosome 12 at 100,558,487 bp
  • T to A, chromosome 13 at 37,947,621 bp
  • T to A, chromosome 13 at 38,655,069 bp
  • T to C, chromosome 13 at 55,663,914 bp
  • T to C, chromosome 14 at 52,312,886 bp
  • T to C, chromosome 14 at 53,294,946 bp
  • T to A, chromosome 14 at 55,238,764 bp
  • T to A, chromosome 15 at 6,745,562 bp
  • G to T, chromosome 15 at 28,346,865 bp
  • A to G, chromosome 15 at 77,788,917 bp
  • A to G, chromosome 15 at 92,770,937 bp
  • T to A, chromosome 15 at 101,782,145 bp
  • T to A, chromosome 16 at 11,805,476 bp
  • A to G, chromosome 16 at 25,876,734 bp
  • T to C, chromosome 16 at 32,052,211 bp
  • T to G, chromosome 16 at 36,759,307 bp
  • A to T, chromosome 16 at 90,791,190 bp
  • C to T, chromosome 16 at 97,356,908 bp
  • C to T, chromosome 17 at 26,218,698 bp
  • T to C, chromosome 17 at 32,659,528 bp
  • A to G, chromosome 17 at 35,156,984 bp
  • T to C, chromosome 17 at 66,436,217 bp
  • A to G, chromosome 17 at 78,755,364 bp
  • T to A, chromosome 18 at 9,214,688 bp
  • C to T, chromosome 18 at 35,622,535 bp
  • T to C, chromosome 18 at 77,339,579 bp
  • C to A, chromosome 19 at 5,719,998 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8324 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
067725-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.