Strain Name:
C57BL/6J-MtgxR8328Btlr/Mmmh
Stock Number:
067727-MU
Citation ID:
RRID:MMRRC_067727-MU
Other Names:
R8328 (G1)
Major Collection:

Strain Information

Rptor
Name: regulatory associated protein of MTOR, complex 1
Synonyms: Rap, 4932417H02Rik, raptor
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74370
Homologene: 80210
Mical3
Name: microtubule associated monooxygenase, calponin and LIM domain containing 3
Synonyms: C130040D16Rik, MICAL-3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 194401
Homologene: 85288
Polr1a
Name: polymerase (RNA) I polypeptide A
Synonyms: Rpo1-4, 3010014K16Rik, RPA194, 194kDa, mRPA1, 2900087K15Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20019
Homologene: 7033
Kdm3b
Name: KDM3B lysine (K)-specific demethylase 3B
Synonyms: JHDM2B, Jmjd1b, 5830462I21Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 277250
VEGA: 18
HGNC: HGNC:1337
Homologene: 41145
C3
Name: complement component 3
Synonyms: Plp, complement factor 3, acylation stimulating protein
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12266
HGNC: HGNC:1318
Homologene: 68031
Hdc
Name: histidine decarboxylase
Synonyms: Hdc-c, L-histidine decarboxylase, Hdc-s, Hdc-e, Hdc-a
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 15186
HGNC: HGNC:4855
Homologene: 20490
Cuzd1
Name: CUB and zona pellucida-like domains 1
Synonyms: UO-44, Itmap1, UTCZP, ERG-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16433
Homologene: 7389
Stn1
Name: STN1, CST complex subunit
Synonyms: 0610009H20Rik, 2310057J23Rik, Obfc1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 108689
Homologene: 11788
Nr4a3
Name: nuclear receptor subfamily 4, group A, member 3
Synonyms: Nor1, MINOR, NOR-1, TEC
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18124
HGNC: HGNC:7982
Homologene: 5074
Wdr19
Name: WD repeat domain 19
Synonyms: DYF2, Ift144, C330027H04Rik, D330023L08Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 213081
Homologene: 11842
Arhgef28
Name: Rho guanine nucleotide exchange factor 28
Synonyms: D13Bwg1089e, Rho specific exchange factor, 9230110L08Rik, RIP2, Rgnef, p190RhoGEF, RhoGEF
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 110596
VEGA: 13
Homologene: 8078
Dennd5b
Name: DENN domain containing 5B
Synonyms: 9330160C06Rik, D030011O10Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320560
Homologene: 44911
Celsr1
Name: cadherin, EGF LAG seven-pass G-type receptor 1
Synonyms: crash, Adgrc1, Crsh, Scy
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12614
VEGA: 15
HGNC: HGNC:1850
Homologene: 7665
Slc9c1
Name: solute carrier family 9, subfamily C (Na+-transporting carboxylic acid decarboxylase), member 1
Synonyms: LOC208169, spermNHE, Slc9a10
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 208169
Homologene: 19505
Ces2a
Name: carboxylesterase 2A
Synonyms: 9130231C15Rik, Ces6
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 102022
HGNC: HGNC:1864
Homologene: 136396
Cyp2j11
Name: cytochrome P450, family 2, subfamily j, polypeptide 11
Synonyms: Cyp2j11-ps
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100066
HGNC: HGNC:2634
Homologene: 133819
Zan
Name: zonadhesin
Synonyms: Zan
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22635
Homologene: 124417
Adamts9
Name: ADAM metallopeptidase with thrombospondin type 1 motif 9
Synonyms: 1810011L16Rik, UND3, Mhdaund4, Gsfund3, 8430403M15Rik, Mhdaund3, E030027K14Rik, UND4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 101401
Homologene: 18821
Atosb
Name: atos homolog B
Synonyms: B230312A22Rik, Fam214b
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230088
Homologene: 32625
Kremen2
Name: kringle containing transmembrane protein 2
Synonyms: Krm2, 2900054E04Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 73016
VEGA: 17
Homologene: 65132
Syde2
Name: synapse defective 1, Rho GTPase, homolog 2 (C. elegans)
Synonyms: C430017H16Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 214804
Homologene: 19035
Dpy19l1
Name: dpy-19 like C-mannosyltransferase 1
Synonyms: 1100001I19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244745
Homologene: 14762
Megf8
Name: multiple EGF-like-domains 8
Synonyms: b2b288Clo, b2b1702Clo, m687Ddg, Egfl4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 269878
HGNC: HGNC:3233
Homologene: 15988
Dsg1b
Name: desmoglein 1 beta
Synonyms: Dsg5
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225256
HGNC: HGNC:3048
Homologene: 1463
Zfp628
Name: zinc finger protein 628
Synonyms: Zec
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232816
Homologene: 72200
Zfp944
Name: zinc finger protein 944
Synonyms: 6330416L07Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 319615
VEGA: 17
Homologene: 133237
Hck
Name: hemopoietic cell kinase
Synonyms: Hck-1, Bmk
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 15162
HGNC: HGNC:4840
Homologene: 20489
Prr5
Name: proline rich 5 (renal)
Synonyms: Protor-1, C030017C09Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 109270
Homologene: 17132
Or4d1
Name: olfactory receptor family 4 subfamily D member 1
Synonyms: GA_x6K02T2PAEV-9555122-9554181, Olfr464, MOR240-2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258407
HGNC: HGNC:8293
Homologene: 44975
Lrrc8e
Name: leucine rich repeat containing 8 family, member E
Synonyms: 1810049O03Rik, C87354
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 72267
Homologene: 11817
Pramel26
Name: PRAME like 26
Synonyms: Gm13084
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 381569
Homologene: 77858
Dsc2
Name: desmocollin 2
Synonyms: Dsc2a, Dsc2b
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13506
HGNC: HGNC:3036
Homologene: 8397
Ankrd6
Name: ankyrin repeat domain 6
Synonyms: diversin
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 140577
Homologene: 8967
Vmn1r209
Name: vomeronasal 1 receptor 209
Synonyms: Gm11315
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 432736
Homologene: 110880
Zscan5b
Name: zinc finger and SCAN domain containing 5B
Synonyms: Zfg1, Zfp371
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 170734
Homologene: 129555
Or9e1
Name: olfactory receptor family 9 subfamily E member 1
Synonyms: MOR222-1, Olfr311, GA_x6K02T2NKPP-565870-564944
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258530
Homologene: 77375
Vmn1r158
Name: vomeronasal 1 receptor 158
Synonyms: Gm16455
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100043067
Homologene: 104166
Ankdd1b
Name: ankyrin repeat and death domain containing 1B
Synonyms: 9330128J19Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 271144
Homologene: 19566
Dmrta2
Name: doublesex and mab-3 related transcription factor like family A2
Synonyms: Dmrt5
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242620
Homologene: 100682
Zfp579
Name: zinc finger protein 579
Synonyms: 1110003A17Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 68490
Homologene: 17629
Nrl
Name: neural retina leucine zipper gene
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18185
VEGA: 14
HGNC: HGNC:8002
Homologene: 4501
Ighv1-23
Name: immunoglobulin heavy variable V1-23
Synonyms: immunoglobulin heavy variable V1-23, Ighv1-23
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 780884
HGNC: HGNC:5553
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 2 at 126,601,883 bp
  • C to T, chromosome 2 at 153,129,067 bp
  • T to C, chromosome 3 at 146,015,741 bp
  • C to T, chromosome 4 at 32,810,215 bp
  • T to C, chromosome 4 at 43,034,751 bp
  • T to A, chromosome 4 at 48,051,323 bp
  • T to A, chromosome 4 at 96,348,368 bp
  • G to T, chromosome 4 at 109,980,009 bp
  • A to G, chromosome 4 at 143,810,810 bp
  • A to G, chromosome 5 at 65,225,295 bp
  • C to T, chromosome 5 at 137,394,464 bp
  • G to A, chromosome 6 at 71,920,734 bp
  • A to T, chromosome 6 at 92,890,012 bp
  • A to G, chromosome 6 at 120,935,177 bp
  • A to G, chromosome 6 at 149,020,617 bp
  • A to G, chromosome 7 at 4,919,814 bp
  • T to G, chromosome 7 at 4,994,867 bp
  • C to T, chromosome 7 at 6,233,947 bp
  • T to C, chromosome 7 at 22,790,062 bp
  • A to G, chromosome 7 at 25,347,492 bp
  • A to T, chromosome 7 at 131,311,616 bp
  • T to A, chromosome 8 at 4,235,641 bp
  • A to T, chromosome 8 at 104,737,366 bp
  • T to A, chromosome 9 at 24,475,390 bp
  • T to A, chromosome 11 at 58,841,634 bp
  • A to G, chromosome 11 at 87,914,159 bp
  • A to G, chromosome 11 at 119,892,647 bp
  • A to G, chromosome 12 at 114,764,496 bp
  • C to A, chromosome 13 at 22,806,473 bp
  • T to A, chromosome 13 at 96,454,866 bp
  • A to T, chromosome 13 at 98,051,009 bp
  • T to C, chromosome 14 at 55,520,706 bp
  • G to C, chromosome 15 at 84,703,186 bp
  • A to G, chromosome 15 at 85,922,244 bp
  • T to A, chromosome 16 at 45,577,864 bp
  • T to A, chromosome 17 at 22,339,724 bp
  • A to G, chromosome 17 at 23,742,771 bp
  • T to C, chromosome 17 at 57,220,973 bp
  • T to C, chromosome 18 at 20,032,519 bp
  • T to A, chromosome 18 at 20,376,950 bp
  • G to A, chromosome 18 at 34,793,070 bp
  • C to A, chromosome 19 at 47,517,059 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8328 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067727-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.