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Strain Name:
C57BL/6J-MtgxR8336Btlr/Mmmh
Stock Number:
067729-MU
Citation ID:
RRID:MMRRC_067729-MU
Other Names:
R8336 (G1)
Major Collection:

Strain Information

Tcf3
Name: transcription factor 3
Synonyms: ALF2, E2A, E47, E12, Pan2, Pan1, A1, Tcfe2a, bHLHb21
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 21423
Homologene: 2408
Kdm5a
Name: lysine demethylase 5A
Synonyms: RBP2, Rbbp2, Jarid1a
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 214899
HGNC: HGNC:9886
Homologene: 3419
Trip12
Name: thyroid hormone receptor interactor 12
Synonyms: Gtl6, 1110036I07Rik, 6720416K24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14897
Homologene: 44226
Cacnb1
Name: calcium channel, voltage-dependent, beta 1 subunit
Synonyms: Cchlb1, Cchb1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12295
HGNC: HGNC:1401
Homologene: 20186
Dusp5
Name: dual specificity phosphatase 5
Synonyms: LOC240672
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 240672
HGNC: HGNC:3071
Homologene: 3256
Nfrkb
Name: nuclear factor related to kappa B binding protein
Synonyms: A530090G11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235134
HGNC: HGNC:7802
Homologene: 4492
Tfap2c
Name: transcription factor AP-2, gamma
Synonyms: Ap-2.2, Stra2, AP2gamma, Tcfap2c
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21420
Homologene: 2423
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 53,206,826 bp
  • A to T, chromosome 1 at 84,766,041 bp
  • T to C, chromosome 2 at 52,273,890 bp
  • C to A, chromosome 2 at 60,422,683 bp
  • A to G, chromosome 2 at 69,712,145 bp
  • T to A, chromosome 2 at 73,274,452 bp
  • A to G, chromosome 2 at 82,990,755 bp
  • A to G, chromosome 2 at 164,032,284 bp
  • T to A, chromosome 2 at 172,557,192 bp
  • A to G, chromosome 3 at 116,772,846 bp
  • A to T, chromosome 5 at 11,846,813 bp
  • T to A, chromosome 5 at 67,315,715 bp
  • T to C, chromosome 6 at 90,302,867 bp
  • A to G, chromosome 6 at 120,419,446 bp
  • C to A, chromosome 7 at 27,673,459 bp
  • C to A, chromosome 7 at 28,814,037 bp
  • A to T, chromosome 7 at 35,427,716 bp
  • C to T, chromosome 7 at 43,776,441 bp
  • C to T, chromosome 7 at 141,984,474 bp
  • A to G, chromosome 8 at 48,293,773 bp
  • T to A, chromosome 8 at 104,739,033 bp
  • C to T, chromosome 8 at 123,892,571 bp
  • T to C, chromosome 9 at 31,403,519 bp
  • T to A, chromosome 9 at 37,537,066 bp
  • T to A, chromosome 9 at 106,075,329 bp
  • A to G, chromosome 10 at 42,696,338 bp
  • A to G, chromosome 10 at 58,272,367 bp
  • T to C, chromosome 10 at 80,421,166 bp
  • A to G, chromosome 10 at 86,691,104 bp
  • A to G, chromosome 10 at 109,767,569 bp
  • G to A, chromosome 11 at 16,257,801 bp
  • A to G, chromosome 11 at 98,003,293 bp
  • C to A, chromosome 12 at 88,368,479 bp
  • T to A, chromosome 13 at 16,857,919 bp
  • C to A, chromosome 13 at 42,155,929 bp
  • A to G, chromosome 13 at 61,356,909 bp
  • G to T, chromosome 13 at 73,821,073 bp
  • C to A, chromosome 14 at 26,711,065 bp
  • T to A, chromosome 14 at 43,727,368 bp
  • T to C, chromosome 14 at 63,986,096 bp
  • T to TCAC, chromosome 15 at 76,107,952 bp
  • T to C, chromosome 15 at 78,540,493 bp
  • T to C, chromosome 16 at 59,167,555 bp
  • A to T, chromosome 17 at 20,570,425 bp
  • A to T, chromosome 17 at 29,148,275 bp
  • T to A, chromosome 18 at 37,515,889 bp
  • A to T, chromosome 18 at 59,007,372 bp
  • T to C, chromosome 19 at 3,815,531 bp
  • A to T, chromosome 19 at 5,054,985 bp
  • T to C, chromosome 19 at 53,540,975 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8336 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067729-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.