Strain Name:
C57BL/6J-MtgxR8336Btlr/Mmmh
Stock Number:
067729-MU
Citation ID:
RRID:MMRRC_067729-MU
Other Names:
R8336 (G1)
Major Collection:

Strain Information

Tcf3
Name: transcription factor 3
Synonyms: Pan2, ALF2, E2A, A1, E47, Tcfe2a, E12, bHLHb21, Pan1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 21423
Homologene: 2408
Kdm5a
Name: lysine demethylase 5A
Synonyms: Jarid1a, RBP2, Rbbp2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 214899
HGNC: HGNC:9886
Homologene: 3419
Trip12
Name: thyroid hormone receptor interactor 12
Synonyms: Gtl6, 1110036I07Rik, 6720416K24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14897
Homologene: 44226
Cacnb1
Name: calcium channel, voltage-dependent, beta 1 subunit
Synonyms: Cchb1, Cchlb1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12295
HGNC: HGNC:1401
Homologene: 20186
Dusp5
Name: dual specificity phosphatase 5
Synonyms: LOC240672
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 240672
HGNC: HGNC:3071
Homologene: 3256
Nfrkb
Name: nuclear factor related to kappa B binding protein
Synonyms: A530090G11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235134
HGNC: HGNC:7802
Homologene: 4492
Tfap2c
Name: transcription factor AP-2, gamma
Synonyms: Stra2, Ap-2.2, AP2gamma, Tcfap2c
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21420
Homologene: 2423
Hnrnpl
Name: heterogeneous nuclear ribonucleoprotein L
Synonyms: Hnrpl, D830027H13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 15388
HGNC: HGNC:5045
Homologene: 1174
Klk11
Name: kallikrein related-peptidase 11
Synonyms: hippostasin, 2310015I08Rik, TLSP, Prss20
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 56538
HGNC: HGNC:6359
Homologene: 27048
Esam
Name: endothelial cell-specific adhesion molecule
Synonyms: W117m, 2310008D05Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69524
Homologene: 12316
Ostm1
Name: osteopetrosis associated transmembrane protein 1
Synonyms: HSPC019, gl, 1200002H13Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14628
Homologene: 32203
Pms1
Name: PMS1 homolog 1, mismatch repair system component
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227099
HGNC: HGNC:9121
Homologene: 449
Tenm3
Name: teneurin transmembrane protein 3
Synonyms: 2610100B16Rik, Odz3, Ten-m3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 23965
Homologene: 22673
Acta1
Name: actin alpha 1, skeletal muscle
Synonyms: Acts, Actsk-1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11459
HGNC: HGNC:129
Homologene: 121702
Sp9
Name: trans-acting transcription factor 9
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 381373
Homologene: 66935
Kmt5b
Name: lysine methyltransferase 5B
Synonyms: C630029K18Rik, Suv4-20h1, Suv420h1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 225888
Homologene: 32351
Dnah12
Name: dynein, axonemal, heavy chain 12
Synonyms: Dnahc12, HL19, DLP12, LOC380889, Dnahc7l, 4921531P07Rik, Hdhc3, DHC3, HL-19
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 110083
HGNC: HGNC:2943
Homologene: 56821
Ces2a
Name: carboxylesterase 2A
Synonyms: 9130231C15Rik, Ces6
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 102022
HGNC: HGNC:1864
Homologene: 136396
Nav3
Name: neuron navigator 3
Synonyms: Pomfil1p, 4732483H20Rik, 9630020C08Rik, steerin 3, unc53H3, POMFIL1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 260315
Homologene: 56688
Agl
Name: amylo-1,6-glucosidase, 4-alpha-glucanotransferase
Synonyms: 9630046L06Rik, 9430004C13Rik, 1110061O17Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 77559
HGNC: HGNC:321
Homologene: 536
Neb
Name: nebulin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241516
Homologene: 110349
Rab44
Name: RAB44, member RAS oncogene family
Synonyms: 9830134C10Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 442827
Homologene: 53300
Sugct
Name: succinyl-CoA glutarate-CoA transferase
Synonyms: 5033411D12Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 192136
VEGA: 13
Homologene: 11681
Map3k10
Name: mitogen-activated protein kinase kinase kinase 10
Synonyms: Mlk2, serine/threonine kinase, MKN28 kinase, mixed lineage kinase 2, MKN28 derived nonreceptor_type
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 269881
HGNC: HGNC:6849
Homologene: 1834
BC048671
Name: cDNA sequence BC048671
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243535
Homologene: 135985
Hivep1
Name: human immunodeficiency virus type I enhancer binding protein 1
Synonyms: alphaA-CRYBP1, Cryabp1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 110521
VEGA: 13
HGNC: HGNC:4920
Homologene: 1596
Adamts19
Name: ADAM metallopeptidase with thrombospondin type 1 motif 19
Synonyms: D230034E10Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240322
VEGA: 18
Homologene: 15860
Col6a4
Name: collagen, type VI, alpha 4
Synonyms: Dvwa, EG235580, Vwa6, 1110001D15Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68553
Homologene: 130754
Pla2r1
Name: phospholipase A2 receptor 1
Synonyms: PLA2-I receptor, Pla2g1br, M-type receptor
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18779
HGNC: HGNC:9042
Homologene: 32016
Adck1
Name: aarF domain containing kinase 1
Synonyms: 2610005A10Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 72113
VEGA: 12
Homologene: 6493
Cep89
Name: centrosomal protein 89
Synonyms: Ccdc123, 2610507L03Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 72140
Homologene: 12444
Vstm2a
Name: V-set and transmembrane domain containing 2A
Synonyms: 9330184N17Rik, Vstm2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 211739
Homologene: 17123
Gcc2
Name: GRIP and coiled-coil domain containing 2
Synonyms: 2600014C01Rik, 0610043A03Rik, 2210420P05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70297
Homologene: 45639
Rin1
Name: Ras and Rab interactor 1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 225870
VEGA: 19
Homologene: 3170
Or5h26
Name: olfactory receptor family 5 subfamily H member 26
Synonyms: MOR183-1, GA_x54KRFPKG5P-55389051-55388122, Olfr196
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 258775
Homologene: 74113
Hsp90b1
Name: heat shock protein 90, beta (Grp94), member 1
Synonyms: tumor rejection antigen (gp96) 1, Tra1, 90 kDa, GRP94, Targ2, ERp99, Tra-1, endoplasmin, gp96
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22027
Homologene: 2476
Pabpc1l
Name: poly(A) binding protein, cytoplasmic 1-like
Synonyms: 1810053B01Rik, ePAB
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 381404
Homologene: 77989
4930578I06Rik
Name: RIKEN cDNA 4930578I06 gene
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 67750
VEGA: 14
Homologene: 49859
Brsk2
Name: BR serine/threonine kinase 2
Synonyms: 4833424K13Rik, SAD-A
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 75770
Homologene: 123829
Sstr3
Name: somatostatin receptor 3
Synonyms: Smstr3, Smstr-3, sst3
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20607
VEGA: 15
Homologene: 20285
Pcdhb21
Name: protocadherin beta 21
Synonyms: PcdhbU, Pcdhb18
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93892
Homologene: 134302
Slc30a9
Name: solute carrier family 30 (zinc transporter), member 9
Synonyms: 2310024J23Rik, GAC63
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 109108
HGNC: HGNC:1329
Homologene: 4627
Fastkd1
Name: FAST kinase domains 1
Synonyms: 5330408N05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 320720
Homologene: 36420
Nkd2
Name: naked cuticle 2
Synonyms: 2210403L10Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 72293
Homologene: 12459
Cts7
Name: cathepsin 7
Synonyms: CTS1, Epcs71, Epcs24
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 56092
VEGA: 13
Homologene: 75110
Gm5145
Name: predicted pseudogene 5145
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 381065
VEGA: 17
Gm16506
Name: predicted gene 16506
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 102634304
Homologene: 128452
Gm6465
Name: predicted gene 6465
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 623998
Eppk1
Name: epiplakin 1
Synonyms: EPPK, EPIPL1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223650
VEGA: 15
Homologene: 20006
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 53,206,826 bp
  • A to T, chromosome 1 at 84,766,041 bp
  • T to C, chromosome 2 at 52,273,890 bp
  • C to A, chromosome 2 at 60,422,683 bp
  • A to G, chromosome 2 at 69,712,145 bp
  • T to A, chromosome 2 at 73,274,452 bp
  • A to G, chromosome 2 at 82,990,755 bp
  • A to G, chromosome 2 at 164,032,284 bp
  • T to A, chromosome 2 at 172,557,192 bp
  • A to G, chromosome 3 at 116,772,846 bp
  • A to T, chromosome 5 at 11,846,813 bp
  • T to A, chromosome 5 at 67,315,715 bp
  • T to C, chromosome 6 at 90,302,867 bp
  • A to G, chromosome 6 at 120,419,446 bp
  • C to A, chromosome 7 at 27,673,459 bp
  • C to A, chromosome 7 at 28,814,037 bp
  • A to T, chromosome 7 at 35,427,716 bp
  • C to T, chromosome 7 at 43,776,441 bp
  • C to T, chromosome 7 at 141,984,474 bp
  • A to G, chromosome 8 at 48,293,773 bp
  • T to A, chromosome 8 at 104,739,033 bp
  • C to T, chromosome 8 at 123,892,571 bp
  • T to C, chromosome 9 at 31,403,519 bp
  • T to A, chromosome 9 at 37,537,066 bp
  • T to A, chromosome 9 at 106,075,329 bp
  • A to G, chromosome 10 at 42,696,338 bp
  • A to G, chromosome 10 at 58,272,367 bp
  • T to C, chromosome 10 at 80,421,166 bp
  • A to G, chromosome 10 at 86,691,104 bp
  • A to G, chromosome 10 at 109,767,569 bp
  • G to A, chromosome 11 at 16,257,801 bp
  • A to G, chromosome 11 at 98,003,293 bp
  • C to A, chromosome 12 at 88,368,479 bp
  • T to A, chromosome 13 at 16,857,919 bp
  • C to A, chromosome 13 at 42,155,929 bp
  • A to G, chromosome 13 at 61,356,909 bp
  • G to T, chromosome 13 at 73,821,073 bp
  • C to A, chromosome 14 at 26,711,065 bp
  • T to A, chromosome 14 at 43,727,368 bp
  • T to C, chromosome 14 at 63,986,096 bp
  • T to TCAC, chromosome 15 at 76,107,952 bp
  • T to C, chromosome 15 at 78,540,493 bp
  • T to C, chromosome 16 at 59,167,555 bp
  • A to T, chromosome 17 at 20,570,425 bp
  • A to T, chromosome 17 at 29,148,275 bp
  • T to A, chromosome 18 at 37,515,889 bp
  • A to T, chromosome 18 at 59,007,372 bp
  • T to C, chromosome 19 at 3,815,531 bp
  • A to T, chromosome 19 at 5,054,985 bp
  • T to C, chromosome 19 at 53,540,975 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8336 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067729-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.