Strain Name:
C57BL/6J-MtgxR8386Btlr/Mmmh
Stock Number:
067752-MU
Citation ID:
RRID:MMRRC_067752-MU
Other Names:
R8386 (G1)
Major Collection:

Strain Information

Wnt7a
Name: wingless-type MMTV integration site family, member 7A
Synonyms: Wnt-7a, tw
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 22421
Homologene: 20969
Cyfip1
Name: cytoplasmic FMR1 interacting protein 1
Synonyms: l(7)1Rl, pl-1, P140SRA-1, E030028J09Rik, Sra-1, Shyc, l7Rl1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20430
Homologene: 22628
Cse1l
Name: chromosome segregation 1 like
Synonyms: Xpo2, Capts, Cas, 2610100P18Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 110750
HGNC: HGNC:2431
Homologene: 1006
Cntnap1
Name: contactin associated protein-like 1
Synonyms: NCP1, Caspr, p190, paranodin, Nrxn4, shm
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 53321
HGNC: HGNC:8011
Homologene: 2693
Casr
Name: calcium-sensing receptor
Synonyms: CaR, cation sensing receptor, Gprc2a
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12374
HGNC: HGNC:1514
Homologene: 332
Aktip
Name: AKT interacting protein
Synonyms: Ft1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14339
Homologene: 7721
Syngap1
Name: synaptic Ras GTPase activating protein 1 homolog (rat)
Synonyms: Syngap
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240057
Homologene: 84739
Slc12a4
Name: solute carrier family 12, member 4
Synonyms: K-Cl Co-transporter-1, KCC1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 20498
Homologene: 21056
Vps13a
Name: vacuolar protein sorting 13A
Synonyms: 4930543C13Rik, 4930516E05Rik, D330038K10Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 271564
VEGA: 19
HGNC: HGNC:1908
Homologene: 22068
Pi16
Name: peptidase inhibitor 16
Synonyms: 1200009H11Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 74116
Homologene: 134317
Cilp
Name: cartilage intermediate layer protein, nucleotide pyrophosphohydrolase
Synonyms: C130036G17Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 214425
HGNC: HGNC:1980
Homologene: 2679
Zfp420
Name: zinc finger protein 420
Synonyms: B230312I18Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233058
Homologene: 129731
Muc5ac
Name: mucin 5, subtypes A and C, tracheobronchial/gastric
Synonyms: MGM, 2210005L13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17833
HGNC: HGNC:7515
Homologene: 137237
Tnik
Name: TRAF2 and NCK interacting kinase
Synonyms: 1500031A17Rik, 4831440I19Rik, C530008O15Rik, C630040K21Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 665113
Homologene: 77943
Setd1b
Name: SET domain containing 1B
Synonyms: KMT2G
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 208043
Homologene: 134654
Ush2a
Name: usherin
Synonyms: Ushrn, A930037M10Rik, MUSH2A, LOC269160, A930011D15Rik, LOC381317, Ush2a
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 22283
Homologene: 66151
Trim80
Name: tripartite motif-containing 80
Synonyms: 4933422H20Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 432613
Homologene: 46325
Arnt2
Name: aryl hydrocarbon receptor nuclear translocator 2
Synonyms: bHLHe1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11864
Homologene: 7230
Abca9
Name: ATP-binding cassette, sub-family A member 9
Synonyms: D630040K07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217262
HGNC: HGNC:39
Homologene: 33332
Adgb
Name: androglobin
Synonyms: 9130014G24Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 215772
Homologene: 100289
Shroom1
Name: shroom family member 1
Synonyms: Shrm1, 1300007L22Rik, Apx
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71774
Homologene: 12411
Shcbp1
Name: Shc SH2-domain binding protein 1
Synonyms: mPAL
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 20419
Homologene: 32123
Ppp1r3c
Name: protein phosphatase 1, regulatory subunit 3C
Synonyms: protein targeting to glicogen, Ppp1r5, PTG
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 53412
VEGA: 19
HGNC: HGNC:9293
Homologene: 3938
Ms4a15
Name: membrane-spanning 4-domains, subfamily A, member 15
Synonyms: E530011F12Rik, LOC383468, Gm1286
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 545279
Homologene: 27391
Fam120b
Name: family with sequence similarity 120, member B
Synonyms: 4932442K08Rik, CCPG
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 67544
VEGA: 17
Homologene: 13033
Or5p70
Name: olfactory receptor family 5 subfamily P member 70
Synonyms: Olfr495, MOR204-37, GA_x6K02T2PBJ9-10725148-10726140
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258361
Homologene: 133600
Sipa1l2
Name: signal-induced proliferation-associated 1 like 2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244668
Homologene: 18956
Zfp882
Name: zinc finger protein 882
Synonyms: ENSMUSG00000052439
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 382019
Homologene: 136303
Fcna
Name: ficolin A
Synonyms: Fcn1, ficolin A
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14133
Homologene: 117949
Or4c11c
Name: olfactory receptor family 4 subfamily C member 11C
Synonyms: Olfr1203, GA_x6K02T2Q125-50336588-50337313, MOR230-1, Olfr1205, GA_x6K02T2Q125-50304328-50305251, MOR230-3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258898
Homologene: 81567
Lin28b
Name: lin-28 homolog B
Synonyms: 2810403D23Rik, D030047M17Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 380669
VEGA: 10
Homologene: 47607
Gast
Name: gastrin
Synonyms: GAS
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14459
HGNC: HGNC:4164
Homologene: 628
Ndufb11b
Name: NADH:ubiquinone oxidoreductase subunit B11B
Synonyms: 1700029P11Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 66346
Homologene: 32635
Malrd1
Name: MAM and LDL receptor class A domain containing 1
Synonyms: Diet1, Gm13364, Gm13318
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 102635496
Homologene: 136214
Igkv6-14
Name: immunoglobulin kappa variable 6-14
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 667881
HGNC: HGNC:5834
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 188,728,206 bp
  • T to A, chromosome 2 at 15,696,844 bp
  • C to T, chromosome 2 at 25,626,015 bp
  • G to A, chromosome 2 at 88,831,289 bp
  • T to C, chromosome 2 at 166,919,684 bp
  • A to G, chromosome 3 at 28,263,674 bp
  • T to A, chromosome 5 at 123,144,256 bp
  • T to C, chromosome 6 at 70,434,982 bp
  • C to T, chromosome 6 at 91,366,288 bp
  • A to T, chromosome 7 at 29,875,618 bp
  • T to C, chromosome 7 at 55,877,740 bp
  • T to A, chromosome 7 at 84,347,539 bp
  • TTTCTTCTTCT to TTTCTTCT, chromosome 7 at 108,395,348 bp
  • G to A, chromosome 7 at 141,807,634 bp
  • A to C, chromosome 8 at 4,767,951 bp
  • A to G, chromosome 8 at 71,914,118 bp
  • G to A, chromosome 8 at 91,131,046 bp
  • A to T, chromosome 8 at 105,951,618 bp
  • A to T, chromosome 8 at 125,492,093 bp
  • G to A, chromosome 9 at 65,279,004 bp
  • A to G, chromosome 10 at 10,350,304 bp
  • A to G, chromosome 10 at 45,469,044 bp
  • A to T, chromosome 11 at 53,466,403 bp
  • A to G, chromosome 11 at 100,336,865 bp
  • A to T, chromosome 11 at 101,182,203 bp
  • G to T, chromosome 11 at 110,130,692 bp
  • A to G, chromosome 11 at 115,445,074 bp
  • C to T, chromosome 15 at 81,980,629 bp
  • T to A, chromosome 16 at 36,515,588 bp
  • G to A, chromosome 17 at 15,422,984 bp
  • T to A, chromosome 17 at 26,960,491 bp
  • C to A, chromosome 17 at 29,319,234 bp
  • A to T, chromosome 19 at 10,993,182 bp
  • A to T, chromosome 19 at 16,701,119 bp
  • T to C, chromosome 19 at 36,733,938 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8386 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067752-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.