Strain Name:
C57BL/6J-MtgxR8395Btlr/Mmmh
Stock Number:
067759-MU
Citation ID:
RRID:MMRRC_067759-MU
Other Names:
R8395 (G1)
Major Collection:

Strain Information

Ippk
Name: inositol 1,3,4,5,6-pentakisphosphate 2-kinase
Synonyms: 1810043M15Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 75678
VEGA: 13
Homologene: 41495
Map4k5
Name: mitogen-activated protein kinase kinase kinase kinase 5
Synonyms: MAPKKKK5, 4432415E19Rik, GCKR, KHS
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 399510
HGNC: HGNC:6867
Homologene: 38199
Limch1
Name: LIM and calponin homology domains 1
Synonyms: 3732412D22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77569
Homologene: 18953
Htr6
Name: 5-hydroxytryptamine (serotonin) receptor 6
Synonyms: 5-HT6
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 15565
HGNC: HGNC:5301
Homologene: 673
Syngr1
Name: synaptogyrin 1
Synonyms: Syngr1b, p29
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20972
Homologene: 3456
Myh10
Name: myosin, heavy polypeptide 10, non-muscle
Synonyms: Myhn2, Fltn, Fltn, NMHC II-B, 5730504C04Rik, nonmuscle myosin heavy chain IIB, 9330167F11Rik, myosin IIB, nonmuscle myosin heavy chain II-B, Myhn-2, SMemb, Myosin IIB, NMHC-B
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 77579
HGNC: HGNC:7568
Homologene: 55941
Ascc3
Name: activating signal cointegrator 1 complex subunit 3
Synonyms: B630009I04Rik, ASC1p200, Helic1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77987
VEGA: 10
Homologene: 4973
Xpo4
Name: exportin 4
Synonyms: B430309A01Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 57258
Homologene: 10733
Gpat4
Name: glycerol-3-phosphate acyltransferase 4
Synonyms: Agpat6
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 102247
Homologene: 32425
Heatr5a
Name: HEAT repeat containing 5A
Synonyms: D930036F22Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 320487
VEGA: 12
Homologene: 19635
Unc45a
Name: unc-45 myosin chaperone A
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101869
Homologene: 32423
Trpm4
Name: transient receptor potential cation channel, subfamily M, member 4
Synonyms: 1110030C19Rik, TRPM4B, LTRPC4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 68667
Homologene: 23033
Ercc8
Name: excision repaiross-complementing rodent repair deficiency, complementation group 8
Synonyms: 2810431L23Rik, B130065P18Rik, 4631412O06Rik, 2410022P04Rik, Csa, Ckn1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 71991
HGNC: HGNC:3439
Homologene: 62
Siglece
Name: sialic acid binding Ig-like lectin E
Synonyms: mSiglec-E, Siglec5, Siglecl1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 83382
Homologene: 130669
Vtcn1
Name: V-set domain containing T cell activation inhibitor 1
Synonyms: B7-H4, B7x
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242122
Homologene: 11627
Neb
Name: nebulin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Abcb9
Name: ATP-binding cassette, sub-family B member 9
Synonyms: TAPL
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56325
HGNC: HGNC:50
Homologene: 10491
Vmn2r25
Name: vomeronasal 2, receptor 25
Synonyms: EG545874
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 545874
Homologene: 135915
Clk3
Name: CDC-like kinase 3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 102414
VEGA: 9
HGNC: HGNC:2071
Homologene: 101534
Vmn2r85
Name: vomeronasal 2, receptor 85
Synonyms: EG623734
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 623734
Homologene: 129606
Zfp217
Name: zinc finger protein 217
Synonyms: 4933431C08Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228913
Homologene: 4757
Dlk2
Name: delta like non-canonical Notch ligand 2
Synonyms: Egfl9
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 106565
Homologene: 11397
Map9
Name: microtubule-associated protein 9
Synonyms: 5033421J10Rik, 5330427D05Rik, ASAP, Mtap9
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 213582
Homologene: 41591
Cenpu
Name: centromere protein U
Synonyms: Mlf1ip, 1700029A22Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 71876
Homologene: 11629
Vmn2r112
Name: vomeronasal 2, receptor 112
Synonyms: EG628185
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 628185
Homologene: 86604
Sgf29
Name: SAGA complex associated factor 29
Synonyms: 1700023O11Rik, Ccdc101
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 75565
Homologene: 12607
Kiz
Name: kizuna centrosomal protein
Synonyms: Plk1s1, Gm114, Ncrna00153, LOC228730
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228730
Homologene: 10219
Mpped1
Name: metallophosphoesterase domain containing 1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223726
HGNC: HGNC:1306
Homologene: 15012
Gbp9
Name: guanylate-binding protein 9
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 236573
Homologene: 128731
Slco2a1
Name: solute carrier organic anion transporter family, member 2a1
Synonyms: mPgt, Slc21a2, Pgt, 2310021C19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 24059
Homologene: 38077
Wdr86
Name: WD repeat domain 86
Synonyms: 2810046M22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 269633
Homologene: 90112
Spata31d1e
Name: spermatogenesis associated 31 subfamily D, member 1E
Synonyms: 1700014D04Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 102638268
Homologene: 140986
Cnn2
Name: calponin 2
Synonyms: h2-calponin, Calpo2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12798
HGNC: HGNC:2156
Homologene: 3215
Or10ad1
Name: olfactory receptor family 10 subfamily AD member 1
Synonyms: Olfr287, MOR286-1, GA_x6K02T2NBG7-5548201-5549154
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 634104
Homologene: 128379
Ubd
Name: ubiquitin D
Synonyms: FAT10, Diubiquitin
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 24108
Homologene: 4665
Slc43a1
Name: solute carrier family 43, member 1
Synonyms: PB39, Pov1, Lat3, 2610016F07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 72401
HGNC: HGNC:9225
Homologene: 2688
Gm3404
Name: predicted gene 3404
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100041554
Homologene: 86127
Or8k18
Name: olfactory receptor family 8 subfamily K member 18
Synonyms: Olfr1049, MOR187-1, GA_x6K02T2Q125-47735396-47734470
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 259018
Homologene: 77387
Bckdk
Name: branched chain ketoacid dehydrogenase kinase
Synonyms: BCKD-kinase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12041
Homologene: 37642
Ighv5-8
Name: immunoglobulin heavy variable V5-8
Synonyms: Gm17309
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 777782
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 2 at 40,657,399 bp
  • T to A, chromosome 2 at 52,175,633 bp
  • C to A, chromosome 2 at 52,257,794 bp
  • T to C, chromosome 2 at 84,840,922 bp
  • G to A, chromosome 2 at 86,255,534 bp
  • A to G, chromosome 2 at 146,953,029 bp
  • G to A, chromosome 2 at 170,119,651 bp
  • A to G, chromosome 3 at 82,381,969 bp
  • T to C, chromosome 3 at 100,883,754 bp
  • T to G, chromosome 4 at 139,061,767 bp
  • A to G, chromosome 5 at 24,730,189 bp
  • A to T, chromosome 5 at 66,969,051 bp
  • A to T, chromosome 5 at 105,080,203 bp
  • T to C, chromosome 5 at 124,080,217 bp
  • T to C, chromosome 5 at 146,525,914 bp
  • T to A, chromosome 6 at 123,823,023 bp
  • A to G, chromosome 7 at 43,656,099 bp
  • T to C, chromosome 7 at 45,309,210 bp
  • A to T, chromosome 7 at 80,326,332 bp
  • T to A, chromosome 7 at 126,672,665 bp
  • G to A, chromosome 7 at 127,907,967 bp
  • GTGTT to GT, chromosome 8 at 23,179,482 bp
  • T to C, chromosome 8 at 46,554,049 bp
  • A to T, chromosome 9 at 57,765,162 bp
  • A to G, chromosome 9 at 103,077,040 bp
  • T to C, chromosome 10 at 50,649,304 bp
  • A to G, chromosome 10 at 79,992,459 bp
  • G to A, chromosome 10 at 130,425,928 bp
  • T to C, chromosome 11 at 68,792,016 bp
  • A to G, chromosome 12 at 51,916,178 bp
  • C to G, chromosome 12 at 69,830,429 bp
  • C to T, chromosome 12 at 113,655,193 bp
  • A to G, chromosome 13 at 49,461,620 bp
  • A to G, chromosome 13 at 59,741,726 bp
  • G to T, chromosome 13 at 108,187,254 bp
  • A to T, chromosome 14 at 57,648,467 bp
  • T to C, chromosome 15 at 80,113,244 bp
  • T to C, chromosome 15 at 83,800,056 bp
  • A to G, chromosome 15 at 98,207,619 bp
  • A to G, chromosome 17 at 22,618,606 bp
  • A to T, chromosome 17 at 37,195,358 bp
  • T to C, chromosome 17 at 46,301,043 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8395 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067759-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.