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Strain Name:
C57BL/6J-MtgxR8294Btlr/Mmmh
Stock Number:
067784-MU
Citation ID:
RRID:MMRRC_067784-MU
Other Names:
R8294 (G1)
Major Collection:

Strain Information

St3gal5
Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 5
Synonyms: ST3Gal V, mST3Gal V, GM3-specific sialytransferase, GM3 synthase, [a]2, 3S-T, Siat9
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20454
Homologene: 2893
Ece1
Name: endothelin converting enzyme 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230857
HGNC: HGNC:3146
Homologene: 1068
Khdc4
Name: KH domain containing 4, pre-mRNA splicing factor
Synonyms: 2810403A07Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74200
Homologene: 41761
Slc44a2
Name: solute carrier family 44, member 2
Synonyms: 1110028E10Rik, CTL2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68682
VEGA: 9
Homologene: 10711
Map3k4
Name: mitogen-activated protein kinase kinase kinase 4
Synonyms: MTK1, MAPKKK4, D17Rp17, RP17, D17Rp17e, Mekk4, T-associated sex reversal, Tas
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 26407
VEGA: 17
HGNC: HGNC:6856
Homologene: 31346
Paip1
Name: polyadenylate binding protein-interacting protein 1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218693
Homologene: 4709
Trim36
Name: tripartite motif-containing 36
Synonyms: D18Wsu100e, Haprin
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 28105
VEGA: 18
Homologene: 10275
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 4,345,997 bp
  • A to G, chromosome 1 at 80,510,362 bp
  • T to A, chromosome 2 at 85,823,187 bp
  • C to T, chromosome 3 at 88,696,608 bp
  • A to G, chromosome 3 at 97,767,378 bp
  • T to C, chromosome 3 at 122,103,568 bp
  • A to G, chromosome 3 at 145,481,089 bp
  • G to T, chromosome 4 at 136,938,586 bp
  • T to C, chromosome 4 at 137,948,620 bp
  • A to T, chromosome 4 at 149,731,097 bp
  • CAT to CATAAT, chromosome 5 at 24,576,591 bp
  • A to T, chromosome 5 at 109,175,112 bp
  • A to G, chromosome 5 at 120,961,366 bp
  • C to T, chromosome 5 at 124,782,346 bp
  • A to C, chromosome 6 at 24,734,379 bp
  • T to A, chromosome 6 at 72,097,832 bp
  • C to T, chromosome 6 at 120,733,786 bp
  • T to C, chromosome 7 at 42,580,265 bp
  • T to C, chromosome 7 at 86,395,279 bp
  • A to T, chromosome 7 at 118,410,005 bp
  • T to C, chromosome 7 at 141,637,350 bp
  • A to G, chromosome 9 at 21,245,004 bp
  • G to T, chromosome 9 at 21,348,347 bp
  • G to A, chromosome 9 at 55,609,996 bp
  • A to T, chromosome 9 at 79,699,312 bp
  • C to G, chromosome 9 at 90,068,436 bp
  • T to G, chromosome 10 at 62,295,845 bp
  • A to T, chromosome 10 at 79,950,701 bp
  • A to G, chromosome 11 at 77,454,201 bp
  • T to A, chromosome 11 at 88,048,641 bp
  • T to A, chromosome 12 at 73,759,710 bp
  • T to A, chromosome 12 at 101,844,901 bp
  • A to G, chromosome 13 at 23,760,974 bp
  • C to T, chromosome 13 at 119,450,764 bp
  • A to G, chromosome 14 at 50,515,626 bp
  • A to C, chromosome 14 at 75,717,179 bp
  • A to T, chromosome 15 at 101,786,037 bp
  • T to C, chromosome 16 at 34,033,584 bp
  • A to G, chromosome 16 at 76,292,530 bp
  • A to T, chromosome 16 at 79,071,288 bp
  • T to A, chromosome 17 at 8,434,532 bp
  • C to A, chromosome 17 at 12,318,613 bp
  • T to C, chromosome 17 at 12,956,356 bp
  • T to C, chromosome 18 at 46,198,521 bp
  • A to G, chromosome 19 at 13,705,646 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8294 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067784-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.