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Strain Name:
C57BL/6J-MtgxR8306Btlr/Mmmh
Stock Number:
067792-MU
Citation ID:
RRID:MMRRC_067792-MU
Other Names:
R8306 (G1)
Major Collection:

Strain Information

Col2a1
Name: collagen, type II, alpha 1
Synonyms: Del1, Col2a-1, Col2a, Col2, M100856, Rgsc856, Lpk, M100413, Rgsc413
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12824
HGNC: HGNC:2200
Homologene: 55607
Notch3
Name: notch 3
Synonyms: hpbk, N3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18131
HGNC: HGNC:7883
Homologene: 376
Rtn4rl1
Name: reticulon 4 receptor-like 1
Synonyms: Ngr3, Ngrh2, Ngrl2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237847
Homologene: 18611
Zmym6
Name: zinc finger, MYM-type 6
Synonyms: D4Wsu24e, 9330177P20Rik, Zfp258
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100177
Homologene: 5186
Zdbf2
Name: zinc finger, DBF-type containing 2
Synonyms: 9330107J05Rik, 4930431J08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73884
Homologene: 52868
Abtb3
Name: ankyrin repeat and BTB domain containing 3
Synonyms: 6330404E16Rik, Btbd11
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74007
Homologene: 72536
Ccdc163
Name: coiled-coil domain containing 163
Synonyms: 4933430J04Rik, 0610037D15Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68394
Homologene: 12265
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 58,991,197 bp
  • T to A, chromosome 1 at 63,304,075 bp
  • T to G, chromosome 1 at 92,491,525 bp
  • T to C, chromosome 1 at 116,821,785 bp
  • G to C, chromosome 1 at 171,383,757 bp
  • T to A, chromosome 2 at 52,209,645 bp
  • A to T, chromosome 2 at 87,783,486 bp
  • G to A, chromosome 2 at 88,831,289 bp
  • T to C, chromosome 2 at 118,457,175 bp
  • T to A, chromosome 2 at 120,422,079 bp
  • T to C, chromosome 2 at 131,246,550 bp
  • T to C, chromosome 2 at 143,950,496 bp
  • A to G, chromosome 3 at 88,965,952 bp
  • A to T, chromosome 3 at 96,197,201 bp
  • C to A, chromosome 3 at 107,473,669 bp
  • A to G, chromosome 3 at 119,412,173 bp
  • A to C, chromosome 4 at 56,886,037 bp
  • T to C, chromosome 4 at 116,710,275 bp
  • A to T, chromosome 4 at 127,122,562 bp
  • A to C, chromosome 4 at 138,277,909 bp
  • A to G, chromosome 4 at 148,158,632 bp
  • T to G, chromosome 5 at 5,604,287 bp
  • G to A, chromosome 5 at 88,459,422 bp
  • G to A, chromosome 5 at 112,307,937 bp
  • T to C, chromosome 5 at 121,503,149 bp
  • G to A, chromosome 5 at 150,536,663 bp
  • T to A, chromosome 6 at 17,637,132 bp
  • T to C, chromosome 6 at 29,449,370 bp
  • T to C, chromosome 6 at 42,358,788 bp
  • T to C, chromosome 6 at 83,151,916 bp
  • T to A, chromosome 7 at 5,453,967 bp
  • T to A, chromosome 7 at 6,480,869 bp
  • C to A, chromosome 7 at 49,546,017 bp
  • T to A, chromosome 7 at 81,624,266 bp
  • T to A, chromosome 7 at 118,722,680 bp
  • C to T, chromosome 7 at 126,948,612 bp
  • T to A, chromosome 7 at 139,656,580 bp
  • A to G, chromosome 8 at 80,612,211 bp
  • A to T, chromosome 8 at 95,747,021 bp
  • G to A, chromosome 9 at 65,279,004 bp
  • A to G, chromosome 9 at 86,520,206 bp
  • A to T, chromosome 9 at 95,920,370 bp
  • A to T, chromosome 9 at 105,784,073 bp
  • A to G, chromosome 9 at 119,521,291 bp
  • C to T, chromosome 10 at 70,553,153 bp
  • C to T, chromosome 10 at 78,200,626 bp
  • T to A, chromosome 10 at 85,598,545 bp
  • G to T, chromosome 10 at 93,482,264 bp
  • T to C, chromosome 10 at 117,002,610 bp
  • T to C, chromosome 11 at 4,408,714 bp
  • T to C, chromosome 11 at 36,069,369 bp
  • G to A, chromosome 11 at 40,721,672 bp
  • T to C, chromosome 11 at 69,059,820 bp
  • T to A, chromosome 11 at 69,777,483 bp
  • T to C, chromosome 11 at 75,265,321 bp
  • C to A, chromosome 11 at 101,411,368 bp
  • T to G, chromosome 11 at 101,525,637 bp
  • T to C, chromosome 11 at 115,013,822 bp
  • T to C, chromosome 12 at 95,779,302 bp
  • G to A, chromosome 14 at 46,884,917 bp
  • A to C, chromosome 14 at 65,979,762 bp
  • T to A, chromosome 15 at 36,161,867 bp
  • T to C, chromosome 15 at 39,878,054 bp
  • A to C, chromosome 15 at 83,555,391 bp
  • T to C, chromosome 15 at 97,990,968 bp
  • A to C, chromosome 16 at 43,618,737 bp
  • A to G, chromosome 17 at 31,473,212 bp
  • G to A, chromosome 17 at 32,158,112 bp
  • A to T, chromosome 17 at 35,321,021 bp
  • A to T, chromosome 17 at 35,342,325 bp
  • G to A, chromosome 18 at 34,628,391 bp
  • A to T, chromosome 18 at 37,022,585 bp
  • A to C, chromosome 18 at 63,075,730 bp
  • T to A, chromosome 19 at 40,815,784 bp
  • C to A, chromosome 19 at 58,536,590 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8306 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067792-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.