Strain Name:
C57BL/6J-MtgxR8422Btlr/Mmmh
Stock Number:
067817-MU
Citation ID:
RRID:MMRRC_067817-MU
Other Names:
R8422 (G1)
Major Collection:

Strain Information

Nedd4
Name: neural precursor cell expressed, developmentally down-regulated 4
Synonyms: Nedd4a, Nedd4-1, Nedd4, E430025J12Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17999
HGNC: HGNC:7727
Homologene: 134533
Ntrk2
Name: neurotrophic tyrosine kinase, receptor, type 2
Synonyms: Tkrb, C030027L06Rik, trkB
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18212
VEGA: 13
HGNC: HGNC:8032
Homologene: 4504
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Aclp7, Acf7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Ptpra
Name: protein tyrosine phosphatase receptor type A
Synonyms: PTPalpha, Ptpa, RPTPalpha, RPTRalpha, PTP[a]
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19262
HGNC: HGNC:9664
Homologene: 20621
Wfs1
Name: wolframin ER transmembrane glycoprotein
Synonyms: Wolfram syndrome 1 homolog (human), wolframin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22393
Homologene: 4380
Dab2ip
Name: disabled 2 interacting protein
Synonyms: AIP1, 2310011D08Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 69601
Homologene: 13058
Dis3l2
Name: DIS3 like 3'-5' exoribonuclease 2
Synonyms: 4930429A22Rik, 8030493P09Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 208718
Homologene: 62417
Ppp1r12a
Name: protein phosphatase 1, regulatory subunit 12A
Synonyms: Mypt1, 5730577I22Rik, D10Ertd625e, 1200015F06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17931
VEGA: 10
HGNC: HGNC:7618
Homologene: 1855
Anapc1
Name: anaphase promoting complex subunit 1
Synonyms: tsg24, Mcpr, 2610021O03Rik, Apc1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17222
Homologene: 7414
4921524J17Rik
Name: RIKEN cDNA 4921524J17 gene
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 66714
Homologene: 12014
Tanc2
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 2
Synonyms: 3526402J09Rik, 5730590C14Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 77097
Homologene: 64680
Cct3
Name: chaperonin containing TCP1 subunit 3
Synonyms: TriC-P5, Tcp1-rs3, Cctg
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 12462
HGNC: HGNC:1616
Homologene: 4373
F2rl3
Name: F2R like thrombin or trypsin receptor 3
Synonyms: PAR4
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14065
HGNC: HGNC:3540
Homologene: 36148
Fgfr3
Name: fibroblast growth factor receptor 3
Synonyms: sam3, HBGFR, Fgfr-3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14184
HGNC: HGNC:3690
Homologene: 55437
C1qtnf5
Name: C1q and tumor necrosis factor related protein 5
Synonyms: CTRP5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235312
Homologene: 9227
Riok3
Name: RIO kinase 3
Synonyms: Sudd, 1200013N13Rik, E130306C24Rik, D18Ertd331e
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 66878
VEGA: 18
Homologene: 2843
Noc3l
Name: NOC3 like DNA replication regulator
Synonyms: Fad24
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 57753
VEGA: 19
Homologene: 39642
Itga11
Name: integrin alpha 11
Synonyms: 4732459H24Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 319480
HGNC: HGNC:6136
Homologene: 8151
Scd2
Name: stearoyl-Coenzyme A desaturase 2
Synonyms: Scd-2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20250
VEGA: 19
Homologene: 136612
Ppp1r3a
Name: protein phosphatase 1, regulatory subunit 3A
Synonyms: GM, RGL
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 140491
HGNC: HGNC:9291
Homologene: 48124
Il12rb2
Name: interleukin 12 receptor, beta 2
Synonyms: A930027I18Rik, Ifnm, IL-12RB2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16162
HGNC: HGNC:5972
Homologene: 1197
Ralgps1
Name: Ral GEF with PH domain and SH3 binding motif 1
Synonyms: RALGEF2, 5830418G11Rik, RALGPS1A
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241308
Homologene: 65163
Rtcb
Name: RNA 2',3'-cyclic phosphate and 5'-OH ligase
Synonyms: D10Wsu52e, HSPC117
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 28088
Homologene: 36344
Npas3
Name: neuronal PAS domain protein 3
Synonyms: 4930423H22Rik, bHLHe12
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 27386
VEGA: 12
Homologene: 8461
Tbx3
Name: T-box 3
Synonyms: D5Ertd189e
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 21386
Homologene: 4371
Ccr3
Name: C-C motif chemokine receptor 3
Synonyms: Cmkbr3, CKR3, MIP-1 alphaRL2, CC-CKR3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12771
VEGA: 9
HGNC: HGNC:1604
Homologene: 20436
Slco6c1
Name: solute carrier organic anion transporter family, member 6c1
Synonyms: 4933404A18Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74441
Homologene: 65259
4930596D02Rik
Name: RIKEN cDNA 4930596D02 gene
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239036
Or10g7
Name: olfactory receptor family 10 subfamily G member 7
Synonyms: MOR223-3, Olfr978, GA_x6K02T2PVTD-33692287-33693222
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 259109
VEGA: 9
Homologene: 88420
3425401B19Rik
Name: RIKEN cDNA 3425401B19 gene
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100504518
VEGA: 14
Homologene: 54908
Or4c107
Name: olfactory receptor family 4 subfamily C member 107
Synonyms: MOR233-17, GA_x6K02T2Q125-50437014-50437949, Olfr1212, MOR233-20
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258241
Homologene: 66154
Ccdc186
Name: coiled-coil domain containing 186
Synonyms: A630007B06Rik, 1810028B20Rik, Otg1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 213993
Homologene: 9963
Rabggta
Name: Rab geranylgeranyl transferase, a subunit
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 56187
VEGA: 14
HGNC: HGNC:9795
Homologene: 32443
Calhm2
Name: calcium homeostasis modulator family member 2
Synonyms: 2810048G17Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 72691
Homologene: 9303
4921517D22Rik
Name: RIKEN cDNA 4921517D22 gene
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 70900
VEGA: 13
Homologene: 87278
Fastkd1
Name: FAST kinase domains 1
Synonyms: 5330408N05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 320720
Homologene: 36420
Tas2r136
Name: taste receptor, type 2, member 136
Synonyms: Tas2r36, mt2r52
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 353165
Homologene: 136304
Clstn2
Name: calsyntenin 2
Synonyms: Cst-2, CS2, 2900042C18Rik, CSTN2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 64085
Homologene: 49698
Nyap1
Name: neuronal tyrosine-phosphorylated phosphoinositide 3-kinase adaptor 1
Synonyms: 6430598A04Rik, Nyap1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243300
Homologene: 18320
Pcdhb20
Name: protocadherin beta 20
Synonyms: Pcdhb14, PcdhbT
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93891
HGNC: HGNC:8685
Homologene: 134303
Plat
Name: plasminogen activator, tissue
Synonyms: t-PA, tPA, D8Ertd2e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18791
HGNC: HGNC:9051
Homologene: 717
Tlr12
Name: toll-like receptor 12
Synonyms: LOC384059, Tlr11
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 384059
Homologene: 135964
Or14a256
Name: olfactory receptor family 14 subfamily A member 256
Synonyms: Olfr294, GA_x6K02T2NHDJ-9504525-9505532, MOR219-5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 257904
Aqp7
Name: aquaporin 7
Synonyms: AQP7L, AQPap
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11832
Homologene: 48000
Or5d47
Name: olfactory receptor family 5 subfamily D member 47
Synonyms: MOR174-4, GA_x6K02T2Q125-49458388-49457432, Olfr74, mOR-EV
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 117005
Homologene: 74078
Zfp810
Name: zinc finger protein 810
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235050
VEGA: 9
Homologene: 138299
Phc3
Name: polyhomeotic 3
Synonyms: HPH3, EDR3, E030046K01Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 241915
Homologene: 69390
Thumpd2
Name: THUMP domain containing 2
Synonyms: 2810025A12Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 72167
VEGA: 17
Homologene: 11898
Imp4
Name: IMP4, U3 small nucleolar ribonucleoprotein
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 27993
Homologene: 68891
Hba-x
Name: hemoglobin X, alpha-like embryonic chain in Hba complex
Synonyms: zeta globin
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15126
HGNC: HGNC:4835
Homologene: 110705
Gm10521
Name: predicted gene 10521
Type: Gene
Species: Mouse
Chromosome: 1
VEGA: 1
Dchs2
Name: dachsous cadherin related 2
Synonyms: LOC229459
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 100534287
Krtap10-30
Name: keratin associated protein 10-30
Synonyms: Gm9736
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77763
VEGA: 10
Homologene: 131747
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 34,443,916 bp
  • A to G, chromosome 1 at 86,854,377 bp
  • T to C, chromosome 1 at 97,125,783 bp
  • A to G, chromosome 1 at 171,896,459 bp
  • T to C, chromosome 2 at 33,172,430 bp
  • T to A, chromosome 2 at 35,707,755 bp
  • A to G, chromosome 2 at 69,702,434 bp
  • T to A, chromosome 2 at 87,973,799 bp
  • T to C, chromosome 2 at 88,958,997 bp
  • T to C, chromosome 2 at 128,675,837 bp
  • T to A, chromosome 2 at 130,532,171 bp
  • G to A, chromosome 3 at 30,929,890 bp
  • T to C, chromosome 3 at 83,325,263 bp
  • G to C, chromosome 3 at 88,300,819 bp
  • T to A, chromosome 4 at 41,035,622 bp
  • C to T, chromosome 4 at 123,409,486 bp
  • A to G, chromosome 4 at 128,616,634 bp
  • T to A, chromosome 5 at 33,734,905 bp
  • T to C, chromosome 5 at 36,973,875 bp
  • G to T, chromosome 5 at 119,680,516 bp
  • T to C, chromosome 5 at 137,735,821 bp
  • G to A, chromosome 6 at 14,718,435 bp
  • A to T, chromosome 6 at 67,360,816 bp
  • A to T, chromosome 6 at 132,777,327 bp
  • T to A, chromosome 7 at 86,616,258 bp
  • G to T, chromosome 8 at 22,772,232 bp
  • G to T, chromosome 8 at 72,763,185 bp
  • T to G, chromosome 8 at 85,412,289 bp
  • C to A, chromosome 9 at 22,283,222 bp
  • T to C, chromosome 9 at 39,994,554 bp
  • C to A, chromosome 9 at 44,108,664 bp
  • A to C, chromosome 9 at 62,767,678 bp
  • T to A, chromosome 9 at 72,742,682 bp
  • T to C, chromosome 9 at 97,458,186 bp
  • T to C, chromosome 9 at 124,028,762 bp
  • C to A, chromosome 10 at 77,750,880 bp
  • C to T, chromosome 10 at 85,943,304 bp
  • T to A, chromosome 10 at 108,241,181 bp
  • A to T, chromosome 11 at 32,277,736 bp
  • A to G, chromosome 11 at 105,835,188 bp
  • C to A, chromosome 12 at 54,068,509 bp
  • A to T, chromosome 13 at 58,985,901 bp
  • T to C, chromosome 13 at 59,691,629 bp
  • G to T, chromosome 14 at 32,662,297 bp
  • G to T, chromosome 14 at 35,810,052 bp
  • G to T, chromosome 14 at 55,718,458 bp
  • A to T, chromosome 17 at 81,026,944 bp
  • A to T, chromosome 18 at 12,136,812 bp
  • A to T, chromosome 18 at 37,504,796 bp
  • T to C, chromosome 19 at 38,807,103 bp
  • G to T, chromosome 19 at 44,301,304 bp
  • T to C, chromosome 19 at 47,133,140 bp
  • G to A, chromosome 19 at 56,813,185 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8422 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067817-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.