Loading...

Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR8425Btlr/Mmmh
Stock Number:
067819-MU
Citation ID:
RRID:MMRRC_067819-MU
Other Names:
R8425 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Nfatc2
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2
Synonyms: NFAT1, NFATp, NFAT1-D
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18019
HGNC: HGNC:7776
Homologene: 7861

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Th
Name: tyrosine hydroxylase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 21823
Homologene: 307

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
P2rx7
Name: purinergic receptor P2X, ligand-gated ion channel, 7
Synonyms: P2X7 receptor, P2X7R, P2X(7)
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18439
HGNC: HGNC:8537
Homologene: 1925

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Aasdh
Name: aminoadipate-semialdehyde dehydrogenase
Synonyms: A230062G08Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231326
Homologene: 71711

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Adgrg1
Name: adhesion G protein-coupled receptor G1
Synonyms: Cyt28, Gpr56
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14766
HGNC: HGNC:4512
Homologene: 4156

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Adam23
Name: a disintegrin and metallopeptidase domain 23
Synonyms: MDC3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23792
HGNC: HGNC:202
Homologene: 2826

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Ddx42
Name: DEAD box helicase 42
Synonyms: B430002H05Rik, 1810047H21Rik, SF3b125, DEAD (Asp-Glu-Ala-Asp) box polypeptide 42
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 72047
Homologene: 49137

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 39,381,409 bp
  • C to T, chromosome 1 at 63,585,377 bp
  • G to A, chromosome 1 at 87,959,836 bp
  • T to A, chromosome 1 at 106,881,785 bp
  • T to A, chromosome 1 at 170,036,016 bp
  • A to G, chromosome 1 at 173,589,860 bp
  • A to T, chromosome 2 at 26,281,536 bp
  • A to T, chromosome 2 at 26,593,654 bp
  • G to A, chromosome 2 at 30,086,501 bp
  • T to A, chromosome 2 at 89,112,393 bp
  • A to T, chromosome 2 at 128,669,868 bp
  • C to T, chromosome 2 at 168,536,296 bp
  • G to T, chromosome 3 at 135,242,627 bp
  • C to T, chromosome 4 at 32,562,316 bp
  • G to A, chromosome 4 at 120,922,363 bp
  • A to G, chromosome 4 at 130,005,057 bp
  • C to A, chromosome 4 at 137,550,867 bp
  • G to T, chromosome 5 at 33,664,530 bp
  • A to T, chromosome 5 at 76,886,277 bp
  • A to G, chromosome 5 at 105,017,774 bp
  • A to G, chromosome 5 at 122,670,458 bp
  • T to G, chromosome 5 at 127,564,357 bp
  • A to T, chromosome 5 at 143,562,839 bp
  • C to T, chromosome 6 at 40,516,118 bp
  • T to C, chromosome 6 at 41,618,646 bp
  • T to A, chromosome 6 at 86,628,869 bp
  • T to C, chromosome 6 at 124,347,786 bp
  • A to G, chromosome 7 at 75,117,599 bp
  • A to T, chromosome 7 at 79,433,541 bp
  • A to G, chromosome 7 at 104,671,295 bp
  • G to T, chromosome 7 at 131,317,991 bp
  • A to T, chromosome 7 at 142,894,086 bp
  • C to T, chromosome 8 at 34,165,448 bp
  • T to C, chromosome 8 at 95,008,407 bp
  • C to T, chromosome 9 at 13,620,117 bp
  • T to A, chromosome 9 at 64,838,974 bp
  • T to A, chromosome 9 at 105,945,957 bp
  • T to C, chromosome 9 at 108,566,983 bp
  • C to A, chromosome 9 at 121,789,102 bp
  • A to T, chromosome 10 at 40,225,433 bp
  • A to T, chromosome 10 at 78,282,377 bp
  • G to T, chromosome 10 at 80,834,982 bp
  • G to T, chromosome 11 at 9,314,623 bp
  • A to G, chromosome 11 at 22,013,495 bp
  • A to G, chromosome 11 at 29,755,008 bp
  • T to G, chromosome 11 at 70,007,847 bp
  • T to A, chromosome 11 at 75,451,522 bp
  • T to C, chromosome 11 at 78,468,645 bp
  • T to C, chromosome 11 at 79,502,000 bp
  • T to A, chromosome 11 at 83,004,615 bp
  • T to C, chromosome 11 at 97,138,851 bp
  • T to C, chromosome 11 at 106,247,724 bp
  • T to A, chromosome 12 at 7,988,842 bp
  • A to G, chromosome 12 at 52,886,621 bp
  • A to T, chromosome 12 at 101,602,769 bp
  • A to G, chromosome 13 at 14,637,336 bp
  • T to A, chromosome 13 at 46,609,732 bp
  • T to A, chromosome 13 at 65,296,675 bp
  • C to T, chromosome 14 at 52,210,555 bp
  • T to A, chromosome 14 at 64,112,560 bp
  • T to C, chromosome 15 at 44,574,515 bp
  • A to T, chromosome 16 at 35,735,597 bp
  • A to G, chromosome 16 at 58,797,603 bp
  • A to G, chromosome 17 at 24,418,169 bp
  • C to A, chromosome 17 at 33,067,064 bp
  • T to C, chromosome 17 at 36,548,757 bp
  • T to A, chromosome 17 at 37,660,084 bp
  • T to C, chromosome 17 at 38,131,036 bp
  • C to T, chromosome 18 at 32,123,358 bp
  • A to C, chromosome 18 at 44,276,003 bp
  • T to C, chromosome 19 at 13,862,485 bp
  • A to G, chromosome 19 at 55,314,444 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8425 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
067819-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.