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Strain Name:
C57BL/6J-MtgxR8515Btlr/Mmmh
Stock Number:
067847-MU
Citation ID:
RRID:MMRRC_067847-MU
Other Names:
R8515 (G1)
Major Collection:

Strain Information

Pcf11
Name: PCF11 cleavage and polyadenylation factor subunit
Synonyms: 5730417B17Rik, 2500001H09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74737
Homologene: 32282
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Fam110b
Name: family with sequence similarity 110, member B
Synonyms: 1700012H17Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242297
Homologene: 17518
Herc4
Name: hect domain and RLD 4
Synonyms: 4921531D01Rik, 1700056O17Rik, 9530080M15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67345
VEGA: 10
Homologene: 56715
Ski
Name: ski sarcoma viral oncogene homolog (avian)
Synonyms: 2610001A11Rik, 2310012I02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 20481
Homologene: 31124
Rab3gap2
Name: RAB3 GTPase activating protein subunit 2
Synonyms: 1110059F07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98732
Homologene: 40842
Wac
Name: WW domain containing adaptor with coiled-coil
Synonyms: Wwp4, 1110067P07Rik, A230035H12Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225131
Homologene: 41148
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 45,912,307 bp
  • A to T, chromosome 1 at 175,647,152 bp
  • G to C, chromosome 1 at 185,262,820 bp
  • C to T, chromosome 2 at 105,389,119 bp
  • G to T, chromosome 2 at 118,689,997 bp
  • G to C, chromosome 2 at 150,472,428 bp
  • C to T, chromosome 2 at 153,316,507 bp
  • T to C, chromosome 2 at 181,574,487 bp
  • A to T, chromosome 3 at 5,399,474 bp
  • T to A, chromosome 3 at 72,929,409 bp
  • A to T, chromosome 3 at 98,883,647 bp
  • T to A, chromosome 3 at 146,458,813 bp
  • T to C, chromosome 4 at 5,799,380 bp
  • C to T, chromosome 4 at 45,803,818 bp
  • ACCAGAAGAGCCATCCAG to ACCAG, chromosome 4 at 46,544,117 bp
  • T to C, chromosome 4 at 112,010,724 bp
  • T to C, chromosome 4 at 155,160,626 bp
  • A to G, chromosome 5 at 30,959,378 bp
  • G to A, chromosome 5 at 115,643,408 bp
  • A to C, chromosome 5 at 139,473,791 bp
  • C to T, chromosome 6 at 83,119,885 bp
  • A to G, chromosome 6 at 86,925,130 bp
  • T to A, chromosome 6 at 123,004,023 bp
  • G to A, chromosome 7 at 66,070,633 bp
  • A to T, chromosome 7 at 80,368,290 bp
  • T to A, chromosome 7 at 92,658,790 bp
  • A to T, chromosome 8 at 23,153,691 bp
  • A to G, chromosome 9 at 70,413,100 bp
  • C to A, chromosome 9 at 115,176,068 bp
  • T to A, chromosome 10 at 63,315,786 bp
  • T to A, chromosome 10 at 82,288,602 bp
  • A to G, chromosome 10 at 106,948,663 bp
  • T to C, chromosome 10 at 128,044,243 bp
  • T to C, chromosome 10 at 128,795,602 bp
  • G to A, chromosome 11 at 34,813,425 bp
  • G to A, chromosome 11 at 51,275,261 bp
  • T to G, chromosome 11 at 53,956,078 bp
  • A to G, chromosome 11 at 68,539,957 bp
  • A to T, chromosome 11 at 107,055,238 bp
  • G to A, chromosome 11 at 115,858,784 bp
  • T to C, chromosome 12 at 4,212,187 bp
  • A to T, chromosome 12 at 76,612,041 bp
  • T to A, chromosome 12 at 81,377,269 bp
  • A to G, chromosome 12 at 81,962,716 bp
  • A to G, chromosome 12 at 83,954,704 bp
  • C to T, chromosome 12 at 117,975,798 bp
  • A to G, chromosome 13 at 23,075,867 bp
  • G to T, chromosome 13 at 65,033,177 bp
  • T to C, chromosome 13 at 114,288,803 bp
  • G to A, chromosome 13 at 119,712,450 bp
  • T to A, chromosome 14 at 20,531,776 bp
  • T to A, chromosome 15 at 81,802,836 bp
  • T to C, chromosome 15 at 82,373,912 bp
  • C to A, chromosome 15 at 89,503,572 bp
  • A to G, chromosome 16 at 15,664,368 bp
  • G to A, chromosome 16 at 32,755,255 bp
  • A to G, chromosome 16 at 36,970,653 bp
  • A to T, chromosome 16 at 87,923,282 bp
  • A to G, chromosome 18 at 7,871,606 bp
  • C to A, chromosome 18 at 12,411,631 bp
  • G to T, chromosome 18 at 34,793,076 bp
  • A to G, chromosome 19 at 8,929,101 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8515 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067847-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.