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Strain Name:
C57BL/6J-MtgxR8316Btlr/Mmmh
Stock Number:
067854-MU
Citation ID:
RRID:MMRRC_067854-MU
Other Names:
R8316 (G1)
Major Collection:

Strain Information

Dnmt3a
Name: DNA methyltransferase 3A
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 13435
HGNC: HGNC:2978
Homologene: 7294
Pdzk1ip1
Name: PDZK1 interacting protein 1
Synonyms: 0610007F13Rik, 2700030M23Rik, Map17
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67182
Homologene: 4213
Rfc1
Name: replication factor C (activator 1) 1
Synonyms: RFC140, Alp145, 140kDa, Recc1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19687
HGNC: HGNC:9969
Homologene: 2187
Usp15
Name: ubiquitin specific peptidase 15
Synonyms: Gcap18, 4921514G19Rik, E430033I05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14479
VEGA: 10
Homologene: 101542
Mical3
Name: microtubule associated monooxygenase, calponin and LIM domain containing 3
Synonyms: MICAL-3, C130040D16Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 194401
Homologene: 85288
Prpf8
Name: pre-mRNA processing factor 8
Synonyms: DBF3/PRP8, Prp8, D11Bwg0410e, Sfprp8l
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 192159
Homologene: 4706
Zmat3
Name: zinc finger matrin type 3
Synonyms: Wig1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 22401
Homologene: 32545
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 58,254,311 bp
  • A to G, chromosome 1 at 106,032,114 bp
  • G to T, chromosome 1 at 188,446,702 bp
  • C to T, chromosome 2 at 6,547,103 bp
  • T to C, chromosome 2 at 31,833,779 bp
  • A to G, chromosome 2 at 69,781,943 bp
  • G to T, chromosome 2 at 157,229,959 bp
  • A to G, chromosome 3 at 28,024,212 bp
  • C to T, chromosome 3 at 29,957,380 bp
  • T to C, chromosome 3 at 32,341,521 bp
  • T to C, chromosome 4 at 24,578,855 bp
  • G to T, chromosome 4 at 48,426,199 bp
  • T to A, chromosome 4 at 115,089,126 bp
  • C to T, chromosome 4 at 149,488,070 bp
  • A to G, chromosome 5 at 8,063,317 bp
  • T to C, chromosome 5 at 65,278,734 bp
  • T to A, chromosome 5 at 86,891,390 bp
  • A to G, chromosome 5 at 90,468,590 bp
  • A to T, chromosome 5 at 110,102,314 bp
  • A to G, chromosome 5 at 129,696,130 bp
  • C to T, chromosome 5 at 149,198,681 bp
  • G to A, chromosome 6 at 48,482,688 bp
  • T to C, chromosome 6 at 120,934,983 bp
  • T to A, chromosome 6 at 141,546,914 bp
  • T to C, chromosome 7 at 19,278,377 bp
  • A to T, chromosome 7 at 104,049,622 bp
  • G to T, chromosome 7 at 107,814,823 bp
  • A to G, chromosome 7 at 108,095,243 bp
  • G to A, chromosome 8 at 21,134,693 bp
  • A to G, chromosome 9 at 71,138,213 bp
  • A to G, chromosome 9 at 111,349,302 bp
  • A to C, chromosome 10 at 4,369,084 bp
  • A to C, chromosome 10 at 79,067,739 bp
  • T to C, chromosome 10 at 97,495,077 bp
  • G to T, chromosome 10 at 123,123,943 bp
  • A to G, chromosome 10 at 129,874,022 bp
  • A to T, chromosome 11 at 72,168,786 bp
  • A to G, chromosome 11 at 75,499,815 bp
  • A to T, chromosome 11 at 98,800,248 bp
  • A to T, chromosome 11 at 115,441,180 bp
  • G to A, chromosome 11 at 115,894,035 bp
  • C to T, chromosome 11 at 120,885,916 bp
  • T to A, chromosome 12 at 3,896,965 bp
  • T to A, chromosome 12 at 8,713,456 bp
  • A to G, chromosome 12 at 26,474,137 bp
  • G to T, chromosome 12 at 30,884,851 bp
  • A to G, chromosome 13 at 14,637,764 bp
  • C to A, chromosome 13 at 75,977,771 bp
  • A to G, chromosome 14 at 45,211,184 bp
  • T to C, chromosome 14 at 61,189,619 bp
  • G to A, chromosome 15 at 4,951,264 bp
  • A to G, chromosome 15 at 31,482,504 bp
  • G to A, chromosome 16 at 21,766,303 bp
  • A to G, chromosome 16 at 37,997,974 bp
  • C to A, chromosome 16 at 49,120,018 bp
  • T to C, chromosome 17 at 13,226,273 bp
  • T to C, chromosome 17 at 27,106,225 bp
  • A to G, chromosome 17 at 36,168,262 bp
  • G to T, chromosome 17 at 46,327,809 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8316 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067854-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.