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Strain Name:
C57BL/6J-MtgxR8341Btlr/Mmmh
Stock Number:
067865-MU
Citation ID:
RRID:MMRRC_067865-MU
Other Names:
R8341 (G1)
Major Collection:

Strain Information

Map2k5
Name: mitogen-activated protein kinase kinase 5
Synonyms: MEK5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 23938
VEGA: 9
HGNC: HGNC:6845
Homologene: 115933
Rbx1
Name: ring-box 1
Synonyms: 1500002P15Rik, ROC1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 56438
VEGA: 15
HGNC: HGNC:9928
Homologene: 134530
Kmt2e
Name: lysine (K)-specific methyltransferase 2E
Synonyms: 1810033J14Rik, D230038D11Rik, 9530077A04Rik, Mll5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 69188
Homologene: 18822
Slc44a2
Name: solute carrier family 44, member 2
Synonyms: 1110028E10Rik, CTL2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68682
VEGA: 9
Homologene: 10711
Thbs3
Name: thrombospondin 3
Synonyms: Thbs-3, TSP3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 21827
Homologene: 5159
Tnks
Name: tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
Synonyms: 4930554K12Rik, D130072O21Rik, tankyrase 1, TANK1, mTNKS1, Parp5a
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 21951
Homologene: 18405
Ints9
Name: integrator complex subunit 9
Synonyms: D14Ertd231e
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 210925
VEGA: 14
Homologene: 10096
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 10,154,328 bp
  • T to C, chromosome 1 at 66,649,033 bp
  • G to A, chromosome 1 at 93,346,278 bp
  • C to A, chromosome 2 at 13,428,724 bp
  • C to T, chromosome 2 at 62,347,890 bp
  • T to C, chromosome 2 at 69,670,524 bp
  • A to T, chromosome 2 at 79,657,718 bp
  • T to C, chromosome 2 at 84,723,773 bp
  • A to G, chromosome 2 at 104,789,475 bp
  • A to G, chromosome 2 at 164,791,885 bp
  • T to A, chromosome 2 at 181,569,719 bp
  • G to A, chromosome 3 at 89,225,391 bp
  • T to C, chromosome 3 at 108,958,592 bp
  • T to A, chromosome 3 at 127,560,915 bp
  • T to C, chromosome 4 at 3,743,304 bp
  • A to G, chromosome 4 at 106,665,696 bp
  • A to G, chromosome 4 at 116,986,792 bp
  • G to T, chromosome 4 at 118,392,836 bp
  • C to G, chromosome 4 at 137,518,979 bp
  • T to C, chromosome 4 at 141,433,396 bp
  • A to T, chromosome 5 at 23,499,453 bp
  • A to C, chromosome 5 at 31,154,103 bp
  • A to T, chromosome 5 at 143,884,937 bp
  • C to T, chromosome 6 at 124,531,156 bp
  • T to C, chromosome 6 at 129,622,700 bp
  • C to A, chromosome 7 at 16,672,003 bp
  • T to C, chromosome 7 at 43,463,954 bp
  • T to A, chromosome 7 at 85,857,920 bp
  • A to G, chromosome 7 at 99,268,440 bp
  • T to A, chromosome 7 at 141,102,334 bp
  • T to A, chromosome 7 at 142,212,119 bp
  • A to T, chromosome 8 at 34,873,045 bp
  • A to G, chromosome 8 at 93,999,178 bp
  • C to T, chromosome 8 at 105,253,865 bp
  • C to T, chromosome 9 at 20,672,622 bp
  • T to C, chromosome 9 at 21,342,199 bp
  • T to C, chromosome 9 at 36,876,309 bp
  • T to A, chromosome 9 at 63,339,098 bp
  • G to A, chromosome 10 at 34,586,283 bp
  • A to C, chromosome 10 at 79,863,211 bp
  • C to T, chromosome 11 at 75,671,427 bp
  • T to G, chromosome 11 at 97,060,163 bp
  • C to A, chromosome 11 at 102,776,536 bp
  • T to A, chromosome 11 at 109,955,050 bp
  • T to A, chromosome 11 at 116,768,888 bp
  • T to A, chromosome 13 at 33,327,307 bp
  • T to C, chromosome 14 at 30,689,881 bp
  • T to A, chromosome 14 at 65,036,414 bp
  • T to C, chromosome 15 at 47,698,151 bp
  • T to C, chromosome 15 at 81,473,877 bp
  • T to C, chromosome 16 at 11,997,151 bp
  • G to T, chromosome 16 at 21,921,584 bp
  • A to T, chromosome 16 at 37,071,771 bp
  • C to T, chromosome 16 at 97,356,908 bp
  • G to A, chromosome 17 at 37,805,652 bp
  • T to C, chromosome 17 at 85,439,580 bp
  • T to C, chromosome 18 at 31,983,926 bp
  • C to T, chromosome 18 at 35,622,535 bp
  • T to G, chromosome 18 at 57,879,209 bp
  • A to T, chromosome 19 at 34,234,001 bp
  • A to T, chromosome X at 89,754,716 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8341 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067865-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.