Strain Name:
C57BL/6J-MtgxR8421Btlr/Mmmh
Stock Number:
067898-MU
Citation ID:
RRID:MMRRC_067898-MU
Other Names:
R8421 (G1)
Major Collection:

Strain Information

Sulf2
Name: sulfatase 2
Synonyms: 2010004N24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 72043
Homologene: 10313
Ide
Name: insulin degrading enzyme
Synonyms: 1300012G03Rik, 4833415K22Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 15925
HGNC: HGNC:5381
Homologene: 3645
Tbx21
Name: T-box 21
Synonyms: Tblym, T-bet, TBT1, Tbet
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 57765
Homologene: 8353
Chrnb3
Name: cholinergic receptor, nicotinic, beta polypeptide 3
Synonyms: 5730417K16Rik, Acrb3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 108043
HGNC: HGNC:1963
Homologene: 36035
Mrpl35
Name: mitochondrial ribosomal protein L35
Synonyms: 1110066C01Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 66223
Homologene: 9598
Nup210l
Name: nucleoporin 210-like
Synonyms: 4930548O11Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 77595
Homologene: 28122
Hcn4
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330953
VEGA: 9
Homologene: 3997
Pard3
Name: par-3 family cell polarity regulator
Synonyms: Par3, D8Ertd580e, PAR-3, ASIP, Pard3a
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 93742
Homologene: 10489
Atad5
Name: ATPase family, AAA domain containing 5
Synonyms: LOC237877, C130052G03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237877
Homologene: 32611
Lin9
Name: lin-9 DREAM MuvB core complex component
Synonyms: 2700022J23Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72568
Homologene: 35252
Gtf3c1
Name: general transcription factor III C 1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233863
HGNC: HGNC:4664
Homologene: 31040
Nav2
Name: neuron navigator 2
Synonyms: 5330421F07Rik, Unc53H2, HELAD1, RAINB2, Rainb1, POMFIL2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 78286
Homologene: 52330
Acin1
Name: apoptotic chromatin condensation inducer 1
Synonyms: 2610036I19Rik, Acinus, 2610510L13Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 56215
Homologene: 22853
Rock1
Name: Rho-associated coiled-coil containing protein kinase 1
Synonyms: Rock-I, 1110055K06Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 19877
VEGA: 18
Homologene: 55899
Zp3
Name: zona pellucida glycoprotein 3
Synonyms: Zp-3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22788
Homologene: 5178
Atp6v1b1
Name: ATPase, H+ transporting, lysosomal V1 subunit B1
Synonyms: D630030L16Rik, D630039P21Rik, lysosomal 56/58kDa, Atp6b1, Vpp3, Vpp-3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 110935
HGNC: HGNC:853
Homologene: 68198
Dync2h1
Name: dynein cytoplasmic 2 heavy chain 1
Synonyms: DHC1b, DHC2, b2b414Clo, Dnchc2, 4432416O06Rik, D330044F14Rik, D030010H02Rik, m152Asp, m407Asp
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 110350
HGNC: HGNC:2962
Homologene: 14468
Ikbkb
Name: inhibitor of kappaB kinase beta
Synonyms: IKK-2, IKK[b], IKK2, IKKbeta, IKK-beta
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 16150
HGNC: HGNC:5960
Homologene: 7782
Plvap
Name: plasmalemma vesicle associated protein
Synonyms: MECA32, PV-1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 84094
Homologene: 10578
Usf3
Name: upstream transcription factor family member 3
Synonyms: 5530400K22Rik, Gm608, LOC207806, LOC385650
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 207806
Homologene: 19504
Unc13b
Name: unc-13 homolog B
Synonyms: Munc13-2, Unc13h2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22249
Homologene: 31376
Slc9c1
Name: solute carrier family 9, subfamily C (Na+-transporting carboxylic acid decarboxylase), member 1
Synonyms: LOC208169, Slc9a10, spermNHE
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 208169
Homologene: 19505
Fat3
Name: FAT atypical cadherin 3
Synonyms: D430038H04Rik, LOC382129, 9430076A06Rik, LOC234973
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270120
VEGA: 9
Homologene: 82252
Myo3a
Name: myosin IIIA
Synonyms: 9030416P08Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 667663
HGNC: HGNC:7601
Homologene: 49486
Cdh16
Name: cadherin 16
Synonyms: KSP-cadherin
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12556
HGNC: HGNC:1755
Homologene: 2997
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Ryr3
Name: ryanodine receptor 3
Synonyms: calcium release channel isoform 3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20192
Homologene: 68151
Atp7b
Name: ATPase, Cu++ transporting, beta polypeptide
Synonyms: Atp7a, Wilson protein, WND
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11979
HGNC: HGNC:870
Homologene: 20063
Zscan20
Name: zinc finger and SCAN domains 20
Synonyms: Zfp31
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269585
Homologene: 51463
Otoa
Name: otoancorin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 246190
Homologene: 71803
Prdm8
Name: PR domain containing 8
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77630
Homologene: 129760
Dglucy
Name: D-glutamate cyclase
Synonyms: 9030617O03Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217830
Homologene: 11798
Mapkbp1
Name: mitogen-activated protein kinase binding protein 1
Synonyms: 2810483F24Rik, Jnkbp1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 26390
Homologene: 69109
Actn3
Name: actinin alpha 3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 11474
HGNC: HGNC:165
Homologene: 862
Ctsll3
Name: cathepsin L-like 3
Synonyms: 2310051M13Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 70202
VEGA: 13
Homologene: 134916
Rasgrf1
Name: RAS protein-specific guanine nucleotide-releasing factor 1
Synonyms: CDC25, Grfbeta, CDC25Mm, Grf1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 19417
HGNC: HGNC:9875
Homologene: 74972
Adcy7
Name: adenylate cyclase 7
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11513
HGNC: HGNC:238
Homologene: 866
Zfp618
Name: zinc finger protein 618
Synonyms: Nedd10, 2810040O04Rik, 2810031P15Rik, D430033D05Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 72701
Homologene: 18975
Padi4
Name: peptidyl arginine deiminase, type IV
Synonyms: Pdi4, PAD type IV, Pad4
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18602
Homologene: 7883
Oxnad1
Name: oxidoreductase NAD-binding domain containing 1
Synonyms: 2410002F01Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218885
Homologene: 16303
Psmb10
Name: proteasome (prosome, macropain) subunit, beta type 10
Synonyms: Mecl-1, Mecl1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19171
HGNC: HGNC:9538
Homologene: 20616
Vmn2r67
Name: vomeronasal 2, receptor 67
Synonyms: EG620672
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 620672
Homologene: 115466
Or8k22
Name: olfactory receptor family 8 subfamily K member 22
Synonyms: MOR188-2, Olfr1054, GA_x6K02T2Q125-47811880-47810942
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 259021
Homologene: 133882
Vmn1r203
Name: vomeronasal 1 receptor 203
Synonyms: V1rh11
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 171270
Homologene: 110880
Potefam1
Name: POTE ankyrin domain family member 1
Synonyms: Pote1, A26c3, 4930430A15Rik, Potea
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67575
Homologene: 69410
Samm50
Name: SAMM50 sorting and assembly machinery component
Synonyms: 1110030L07Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 68653
VEGA: 15
Homologene: 41034
Ankrd40
Name: ankyrin repeat domain 40
Synonyms: 5530600A18Rik, 1110011C06Rik, Gcap15
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71452
Homologene: 12393
Dnase1l3
Name: deoxyribonuclease 1-like 3
Synonyms: DNasegamma
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13421
HGNC: HGNC:2959
Homologene: 3630
Marveld3
Name: MARVEL (membrane-associating) domain containing 3
Synonyms: Mrvldc3, MARVD3, 1810006A16Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 73608
Homologene: 12504
Mrpl1
Name: mitochondrial ribosomal protein L1
Synonyms: 5830418D04Rik, 2410002L03Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 94061
Homologene: 41355
Prss21
Name: serine protease 21
Synonyms: TESP5, 1700023E12Rik, mT4, testisin
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 57256
HGNC: HGNC:9485
Homologene: 137342
Csf1r
Name: colony stimulating factor 1 receptor
Synonyms: CD115, Fms, Fim2, M-CSFR, CSF-1R, Csfmr, Fim-2
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 12978
HGNC: HGNC:2433
Homologene: 3817
Ube2q2l
Name: ubiquitin conjugating enzyme E2 Q2 like
Synonyms: E330021D16Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 100502936
Ankef1
Name: ankyrin repeat and EF-hand domain containing 1
Synonyms: Ankrd5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 319196
Homologene: 11130
Ccdc87
Name: coiled-coil domain containing 87
Synonyms: 4931419P11Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 399599
VEGA: 19
Homologene: 49531
Vmn1r114
Name: vomeronasal 1 receptor 114
Synonyms: Gm10669
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100042996
Homologene: 104166
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 180,665,800 bp
  • T to C, chromosome 2 at 22,362,124 bp
  • T to A, chromosome 2 at 40,725,423 bp
  • T to A, chromosome 2 at 86,332,903 bp
  • T to A, chromosome 2 at 111,218,610 bp
  • T to C, chromosome 2 at 112,996,584 bp
  • C to A, chromosome 2 at 120,018,950 bp
  • C to A, chromosome 2 at 136,537,165 bp
  • T to A, chromosome 2 at 166,117,052 bp
  • A to G, chromosome 3 at 90,203,867 bp
  • A to G, chromosome 4 at 43,178,304 bp
  • A to G, chromosome 4 at 63,133,246 bp
  • T to C, chromosome 4 at 128,585,827 bp
  • C to A, chromosome 4 at 140,748,222 bp
  • C to G, chromosome 5 at 96,226,367 bp
  • C to T, chromosome 5 at 98,185,963 bp
  • A to G, chromosome 5 at 135,988,477 bp
  • T to C, chromosome 6 at 71,816,167 bp
  • T to A, chromosome 6 at 83,753,809 bp
  • T to C, chromosome 6 at 136,401,352 bp
  • A to T, chromosome 7 at 20,811,534 bp
  • A to G, chromosome 7 at 49,452,521 bp
  • A to C, chromosome 7 at 85,136,685 bp
  • G to A, chromosome 7 at 121,099,268 bp
  • A to G, chromosome 7 at 125,698,970 bp
  • A to T, chromosome 8 at 22,028,471 bp
  • A to T, chromosome 8 at 22,678,788 bp
  • G to A, chromosome 8 at 27,396,690 bp
  • G to A, chromosome 8 at 71,511,532 bp
  • A to G, chromosome 8 at 88,322,184 bp
  • C to T, chromosome 8 at 104,621,970 bp
  • G to T, chromosome 8 at 105,936,710 bp
  • A to G, chromosome 8 at 109,948,647 bp
  • A to C, chromosome 8 at 127,140,408 bp
  • T to C, chromosome 9 at 7,102,477 bp
  • A to T, chromosome 9 at 15,998,184 bp
  • T to A, chromosome 9 at 58,858,096 bp
  • C to T, chromosome 9 at 89,967,915 bp
  • T to A, chromosome 11 at 80,094,558 bp
  • G to T, chromosome 11 at 94,334,836 bp
  • T to C, chromosome 11 at 97,114,735 bp
  • A to G, chromosome 12 at 100,842,679 bp
  • T to G, chromosome 13 at 22,524,984 bp
  • A to G, chromosome 13 at 60,800,781 bp
  • T to C, chromosome 14 at 7,968,122 bp
  • T to G, chromosome 14 at 32,099,474 bp
  • T to C, chromosome 14 at 54,643,029 bp
  • A to G, chromosome 15 at 84,210,585 bp
  • A to T, chromosome 16 at 44,217,209 bp
  • A to G, chromosome 16 at 45,593,371 bp
  • T to A, chromosome 17 at 23,869,368 bp
  • G to A, chromosome 18 at 10,072,863 bp
  • A to G, chromosome 18 at 61,127,894 bp
  • T to C, chromosome 19 at 4,841,285 bp
  • T to C, chromosome 19 at 4,861,713 bp
  • A to G, chromosome 19 at 37,278,004 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8421 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067898-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.