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Strain Name:
C57BL/6J-MtgxR8423Btlr/Mmmh
Stock Number:
067899-MU
Citation ID:
RRID:MMRRC_067899-MU
Other Names:
R8423 (G1)
Major Collection:

Strain Information

Tnrc6b
Name: trinucleotide repeat containing 6b
Synonyms: A730065C02Rik, D230019K20Rik, 2700090M07Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 213988
VEGA: 15
Homologene: 66194
Knl1
Name: kinetochore scaffold 1
Synonyms: 2310043D08Rik, 5730505K17Rik, Casc5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 76464
Homologene: 44890
Miga1
Name: mitoguardin 1
Synonyms: C030011O14Rik, Mita1, Fam73a
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 215708
Homologene: 18369
Mynn
Name: myoneurin
Synonyms: SBBIZ1, 2810011C24Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80732
Homologene: 10253
Dmxl1
Name: Dmx-like 1
Synonyms: C630007L23Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240283
HGNC: HGNC:2937
Homologene: 21136
Bub1
Name: BUB1, mitotic checkpoint serine/threonine kinase
Synonyms: Bub1a, D2Xrf87
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12235
HGNC: HGNC:1148
Homologene: 37910
Herc1
Name: HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Synonyms: D130015N03Rik, 2810449H11Rik, tbl
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235439
HGNC: HGNC:4867
Homologene: 31207
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 24,021,917 bp
  • C to A, chromosome 1 at 53,472,904 bp
  • T to A, chromosome 1 at 60,150,593 bp
  • T to C, chromosome 1 at 92,907,184 bp
  • A to T, chromosome 2 at 89,617,987 bp
  • T to G, chromosome 2 at 119,070,032 bp
  • G to A, chromosome 2 at 127,807,830 bp
  • A to G, chromosome 2 at 180,998,705 bp
  • A to G, chromosome 3 at 30,603,784 bp
  • T to C, chromosome 3 at 103,080,989 bp
  • T to G, chromosome 3 at 129,309,125 bp
  • A to T, chromosome 3 at 152,322,408 bp
  • T to C, chromosome 4 at 43,770,598 bp
  • C to T, chromosome 4 at 116,222,375 bp
  • C to T, chromosome 4 at 135,917,516 bp
  • A to G, chromosome 5 at 38,794,910 bp
  • T to A, chromosome 5 at 105,119,934 bp
  • G to A, chromosome 5 at 105,480,756 bp
  • A to G, chromosome 5 at 110,596,245 bp
  • G to A, chromosome 5 at 111,233,341 bp
  • G to A, chromosome 6 at 57,002,510 bp
  • T to A, chromosome 6 at 124,574,658 bp
  • A to G, chromosome 7 at 24,292,034 bp
  • T to C, chromosome 7 at 100,160,243 bp
  • T to A, chromosome 7 at 109,475,392 bp
  • G to T, chromosome 8 at 22,772,232 bp
  • C to A, chromosome 9 at 20,638,864 bp
  • T to C, chromosome 9 at 66,508,160 bp
  • C to T, chromosome 9 at 92,490,790 bp
  • C to T, chromosome 9 at 111,468,719 bp
  • T to C, chromosome 10 at 33,622,859 bp
  • A to T, chromosome 11 at 69,354,426 bp
  • T to C, chromosome 11 at 74,121,666 bp
  • A to G, chromosome 12 at 55,659,062 bp
  • A to G, chromosome 12 at 83,980,330 bp
  • A to G, chromosome 12 at 113,490,910 bp
  • A to T, chromosome 13 at 40,719,230 bp
  • T to A, chromosome 13 at 73,665,574 bp
  • A to G, chromosome 13 at 112,988,144 bp
  • T to A, chromosome 14 at 51,571,613 bp
  • A to G, chromosome 14 at 55,499,065 bp
  • A to T, chromosome 14 at 55,705,643 bp
  • G to A, chromosome 15 at 80,929,418 bp
  • G to A, chromosome 15 at 102,954,742 bp
  • A to G, chromosome 16 at 10,576,663 bp
  • A to T, chromosome 16 at 36,970,585 bp
  • T to C, chromosome 17 at 75,292,857 bp
  • G to T, chromosome 18 at 3,123,365 bp
  • T to C, chromosome 18 at 49,865,116 bp
  • T to C, chromosome 19 at 41,585,449 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8423 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067899-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.