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Strain Name:
C57BL/6J-MtgxR8466Btlr/Mmmh
Stock Number:
067910-MU
Citation ID:
RRID:MMRRC_067910-MU
Other Names:
R8466 (G1)
Major Collection:

Strain Information

Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Cep70
Name: centrosomal protein 70
Synonyms: 6720484E09Rik, C030018L16Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68121
Homologene: 11387
Utp20
Name: UTP20 small subunit processome component
Synonyms: mDRIM, DRIM, 3830408P06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70683
VEGA: 10
Homologene: 38373
Cwc27
Name: CWC27 spliceosome-associated protein
Synonyms: NY-CO-10, 3110009E13Rik, Sdccag10
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 67285
Homologene: 21192
Usp48
Name: ubiquitin specific peptidase 48
Synonyms: D330022K21Rik, 2810449C13Rik, Usp31
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 170707
Homologene: 12988
Stat3
Name: signal transducer and activator of transcription 3
Synonyms: Aprf, 1110034C02Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20848
Homologene: 7960
Akap9
Name: A kinase anchor protein 9
Synonyms: AKAP450, 5730481H23Rik, G1-448-15, repro12, mei2-5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100986
HGNC: HGNC:379
Homologene: 134583
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 2 at 52,464,667 bp
  • T to C, chromosome 2 at 120,300,482 bp
  • A to C, chromosome 2 at 121,510,557 bp
  • A to T, chromosome 3 at 5,242,702 bp
  • C to T, chromosome 3 at 89,751,159 bp
  • T to A, chromosome 3 at 92,014,634 bp
  • T to C, chromosome 4 at 99,064,099 bp
  • T to C, chromosome 4 at 123,455,444 bp
  • T to A, chromosome 4 at 137,623,319 bp
  • T to A, chromosome 4 at 151,086,120 bp
  • G to A, chromosome 5 at 4,038,659 bp
  • C to A, chromosome 5 at 36,484,908 bp
  • A to T, chromosome 5 at 83,280,520 bp
  • G to C, chromosome 5 at 115,327,433 bp
  • A to G, chromosome 6 at 29,438,622 bp
  • C to A, chromosome 6 at 59,354,481 bp
  • T to A, chromosome 6 at 59,354,482 bp
  • C to T, chromosome 6 at 147,107,743 bp
  • A to G, chromosome 7 at 10,050,070 bp
  • T to A, chromosome 7 at 64,372,486 bp
  • C to A, chromosome 7 at 98,450,030 bp
  • C to T, chromosome 8 at 122,620,886 bp
  • G to A, chromosome 8 at 123,489,909 bp
  • A to T, chromosome 8 at 125,492,246 bp
  • T to C, chromosome 9 at 18,643,148 bp
  • T to A, chromosome 9 at 71,252,923 bp
  • T to C, chromosome 9 at 99,278,020 bp
  • A to T, chromosome 10 at 88,818,503 bp
  • A to G, chromosome 10 at 100,602,011 bp
  • A to T, chromosome 11 at 73,965,087 bp
  • A to G, chromosome 11 at 74,240,190 bp
  • A to G, chromosome 11 at 74,425,231 bp
  • G to A, chromosome 11 at 75,658,387 bp
  • A to T, chromosome 11 at 100,895,098 bp
  • T to C, chromosome 12 at 64,986,442 bp
  • A to C, chromosome 12 at 83,778,481 bp
  • G to T, chromosome 12 at 103,409,901 bp
  • T to A, chromosome 13 at 38,023,011 bp
  • T to C, chromosome 13 at 97,098,984 bp
  • G to T, chromosome 13 at 104,804,264 bp
  • C to A, chromosome 13 at 104,804,268 bp
  • A to T, chromosome 14 at 20,710,676 bp
  • T to A, chromosome 14 at 55,072,896 bp
  • T to C, chromosome 16 at 30,582,101 bp
  • A to G, chromosome 16 at 85,802,512 bp
  • T to C, chromosome 17 at 23,996,828 bp
  • G to T, chromosome 17 at 35,094,260 bp
  • G to A, chromosome 17 at 36,548,093 bp
  • G to A, chromosome 17 at 67,813,953 bp
  • C to A, chromosome 18 at 23,038,581 bp
  • A to T, chromosome 19 at 17,572,500 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8466 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067910-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.