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Strain Name:
C57BL/6J-MtgxR8477Btlr/Mmmh
Stock Number:
067921-MU
Citation ID:
RRID:MMRRC_067921-MU
Other Names:
R8477 (G1)
Major Collection:

Strain Information

Wasf2
Name: WASP family, member 2
Synonyms: D4Ertd13e, WAVE2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242687
Homologene: 86743
Limch1
Name: LIM and calponin homology domains 1
Synonyms: 3732412D22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77569
Homologene: 18953
Dpagt1
Name: dolichyl-phosphate N-acetylglucosaminephosphotransferase 1
Synonyms: GPT, Dpagt2, Gnpta
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13478
VEGA: 9
HGNC: HGNC:2995
Homologene: 1058
U2af2
Name: U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22185
Homologene: 110853
Mast2
Name: microtubule associated serine/threonine kinase 2
Synonyms: MAST205, Mtssk
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17776
Homologene: 7428
Csnk1g1
Name: casein kinase 1, gamma 1
Synonyms: 9130020E21Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 214897
HGNC: HGNC:2454
Homologene: 48692
Polr2a
Name: polymerase (RNA) II (DNA directed) polypeptide A
Synonyms: 220kDa, Rpo2-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20020
HGNC: HGNC:9187
Homologene: 721
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 116,803,124 bp
  • C to T, chromosome 1 at 173,332,188 bp
  • C to T, chromosome 1 at 181,816,974 bp
  • A to T, chromosome 1 at 189,653,188 bp
  • A to T, chromosome 2 at 34,989,170 bp
  • T to C, chromosome 2 at 37,005,048 bp
  • A to C, chromosome 2 at 127,539,204 bp
  • T to C, chromosome 3 at 87,084,831 bp
  • C to T, chromosome 4 at 43,569,944 bp
  • G to T, chromosome 4 at 43,891,011 bp
  • A to G, chromosome 4 at 62,192,003 bp
  • C to T, chromosome 4 at 116,307,210 bp
  • A to G, chromosome 4 at 133,185,101 bp
  • A to T, chromosome 4 at 148,565,390 bp
  • G to A, chromosome 4 at 150,125,662 bp
  • A to T, chromosome 5 at 23,513,988 bp
  • G to A, chromosome 5 at 66,974,565 bp
  • A to C, chromosome 5 at 95,801,708 bp
  • C to T, chromosome 5 at 106,881,818 bp
  • C to CTCT, chromosome 6 at 4,756,453 bp
  • A to T, chromosome 6 at 52,640,200 bp
  • G to T, chromosome 6 at 54,269,482 bp
  • T to A, chromosome 6 at 115,935,385 bp
  • T to C, chromosome 6 at 124,224,241 bp
  • T to C, chromosome 6 at 137,056,186 bp
  • T to C, chromosome 7 at 5,075,694 bp
  • T to C, chromosome 7 at 26,459,794 bp
  • T to A, chromosome 7 at 30,728,231 bp
  • T to C, chromosome 7 at 105,898,926 bp
  • A to G, chromosome 7 at 127,300,933 bp
  • A to G, chromosome 9 at 21,932,927 bp
  • A to T, chromosome 9 at 39,257,803 bp
  • G to T, chromosome 9 at 44,332,093 bp
  • T to C, chromosome 9 at 66,002,273 bp
  • A to G, chromosome 10 at 7,705,515 bp
  • G to A, chromosome 10 at 79,088,340 bp
  • G to A, chromosome 10 at 83,501,921 bp
  • A to G, chromosome 10 at 114,800,717 bp
  • A to T, chromosome 10 at 127,184,397 bp
  • A to G, chromosome 10 at 127,484,160 bp
  • G to A, chromosome 11 at 69,735,486 bp
  • T to C, chromosome 11 at 79,516,065 bp
  • T to A, chromosome 11 at 107,052,853 bp
  • G to A, chromosome 12 at 115,067,580 bp
  • A to G, chromosome 13 at 3,575,079 bp
  • A to G, chromosome 13 at 12,601,783 bp
  • A to T, chromosome 13 at 42,184,220 bp
  • T to A, chromosome 13 at 58,576,795 bp
  • C to A, chromosome 14 at 50,917,877 bp
  • T to C, chromosome 14 at 118,901,546 bp
  • T to C, chromosome 15 at 27,773,952 bp
  • A to T, chromosome 15 at 78,485,806 bp
  • A to G, chromosome 16 at 4,687,348 bp
  • A to T, chromosome 17 at 66,377,947 bp
  • A to C, chromosome 17 at 70,516,972 bp
  • T to A, chromosome 18 at 37,505,254 bp
  • C to A, chromosome 18 at 37,763,312 bp
  • A to T, chromosome 18 at 65,624,066 bp
  • A to G, chromosome 19 at 29,597,783 bp
  • C to A, chromosome 19 at 34,878,655 bp
  • G to T, chromosome 19 at 39,011,041 bp
  • T to A, chromosome 19 at 40,070,264 bp
  • C to A, chromosome X at 96,714,017 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8477 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067921-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.