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Strain Name:
C57BL/6J-MtgxR8478Btlr/Mmmh
Stock Number:
067922-MU
Citation ID:
RRID:MMRRC_067922-MU
Other Names:
R8478 (G1)
Major Collection:

Strain Information

Pou2f1
Name: POU domain, class 2, transcription factor 1
Synonyms: Oct-1C, Oct-1B, Oct-1A, oct-1, Otf-1, Otf1, 2810482H01Rik, Oct-1z, Oct1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18986
HGNC: HGNC:9212
Homologene: 37658
Utrn
Name: utrophin
Synonyms: G-utrophin, Dmdl, DRP
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398
Cacna1g
Name: calcium channel, voltage-dependent, T type, alpha 1G subunit
Synonyms: a1G, Cav3.1d
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12291
HGNC: HGNC:1394
Homologene: 22544
Nub1
Name: negative regulator of ubiquitin-like proteins 1
Synonyms: BS4, NY-REN-18, 4931404D21Rik, 6330412F12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 53312
Homologene: 41108
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Smyd1
Name: SET and MYND domain containing 1
Synonyms: Bop, 4632404M21Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12180
Homologene: 7645
Csrnp3
Name: cysteine-serine-rich nuclear protein 3
Synonyms: mbu1, A330102K23Rik, CSRNP-3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 77771
Homologene: 11803
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 36,551,790 bp
  • T to C, chromosome 1 at 59,186,016 bp
  • C to A, chromosome 1 at 151,636,512 bp
  • A to T, chromosome 1 at 165,931,718 bp
  • G to A, chromosome 1 at 188,443,232 bp
  • A to T, chromosome 2 at 65,878,056 bp
  • A to T, chromosome 2 at 125,929,585 bp
  • A to G, chromosome 3 at 32,451,848 bp
  • A to T, chromosome 3 at 37,033,277 bp
  • A to T, chromosome 3 at 64,116,836 bp
  • T to C, chromosome 3 at 83,898,462 bp
  • G to A, chromosome 3 at 96,716,750 bp
  • T to A, chromosome 3 at 127,729,312 bp
  • A to G, chromosome 3 at 157,546,108 bp
  • A to T, chromosome 4 at 118,484,782 bp
  • A to G, chromosome 4 at 149,473,384 bp
  • C to T, chromosome 4 at 152,356,690 bp
  • A to G, chromosome 5 at 5,736,432 bp
  • G to T, chromosome 5 at 24,701,424 bp
  • G to T, chromosome 5 at 81,794,501 bp
  • C to T, chromosome 5 at 88,615,036 bp
  • A to T, chromosome 5 at 108,995,770 bp
  • T to A, chromosome 5 at 110,252,952 bp
  • G to T, chromosome 5 at 120,560,321 bp
  • T to C, chromosome 5 at 147,879,153 bp
  • C to T, chromosome 6 at 5,185,318 bp
  • T to A, chromosome 6 at 48,540,097 bp
  • A to T, chromosome 6 at 71,216,827 bp
  • A to G, chromosome 6 at 87,581,348 bp
  • G to T, chromosome 6 at 90,108,089 bp
  • A to G, chromosome 6 at 108,783,324 bp
  • T to C, chromosome 7 at 12,606,354 bp
  • A to G, chromosome 7 at 46,221,433 bp
  • G to T, chromosome 7 at 49,461,985 bp
  • C to T, chromosome 7 at 66,171,000 bp
  • C to A, chromosome 7 at 104,939,270 bp
  • A to G, chromosome 7 at 105,682,794 bp
  • A to T, chromosome 7 at 116,418,349 bp
  • A to G, chromosome 7 at 126,448,330 bp
  • A to T, chromosome 8 at 60,919,488 bp
  • T to A, chromosome 8 at 80,611,558 bp
  • C to T, chromosome 8 at 109,961,968 bp
  • T to C, chromosome 8 at 128,761,369 bp
  • C to T, chromosome 9 at 4,793,882 bp
  • T to G, chromosome 9 at 88,723,435 bp
  • A to T, chromosome 9 at 107,928,645 bp
  • A to G, chromosome 10 at 4,148,064 bp
  • A to G, chromosome 10 at 12,649,148 bp
  • A to G, chromosome 10 at 128,671,052 bp
  • A to G, chromosome 11 at 5,962,407 bp
  • A to G, chromosome 11 at 24,165,086 bp
  • T to C, chromosome 11 at 57,309,842 bp
  • A to T, chromosome 11 at 70,394,320 bp
  • A to G, chromosome 11 at 76,274,043 bp
  • A to G, chromosome 11 at 94,426,452 bp
  • A to T, chromosome 12 at 55,600,545 bp
  • A to G, chromosome 12 at 114,612,299 bp
  • T to C, chromosome 15 at 76,363,429 bp
  • T to A, chromosome 16 at 15,648,924 bp
  • T to C, chromosome 16 at 63,773,081 bp
  • T to C, chromosome 17 at 14,680,404 bp
  • G to T, chromosome 17 at 18,452,282 bp
  • C to A, chromosome 17 at 73,906,058 bp
  • T to C, chromosome 18 at 38,854,891 bp
  • C to T, chromosome 19 at 4,426,691 bp
  • T to C, chromosome 19 at 5,454,919 bp
  • T to C, chromosome 19 at 47,776,076 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8478 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067922-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.