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Strain Name:
C57BL/6J-MtgxR8479Btlr/Mmmh
Stock Number:
067923-MU
Citation ID:
RRID:MMRRC_067923-MU
Other Names:
R8479 (G1)
Major Collection:

Strain Information

Cntnap2
Name: contactin associated protein-like 2
Synonyms: 5430425M22Rik, Caspr2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 66797
Homologene: 69159
Met
Name: met proto-oncogene
Synonyms: HGF receptor, c-Met, Par4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17295
HGNC: HGNC:7029
Homologene: 206
Ess2
Name: ess-2 splicing factor
Synonyms: ES2, Dgsi, D16H22S1269E, Es2el, Dgcr14
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 27886
VEGA: 16
Homologene: 11184
Col18a1
Name: collagen, type XVIII, alpha 1
Synonyms: endostatin
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12822
HGNC: HGNC:2195
Homologene: 7673
Mapkap1
Name: mitogen-activated protein kinase associated protein 1
Synonyms: D230039K05Rik, Sin1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227743
Homologene: 11473
Dido1
Name: death inducer-obliterator 1
Synonyms: DIO-1, 6720461J16Rik, D130048F08Rik, Datf1, dido, C130092D22Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 23856
HGNC: HGNC:2680
Homologene: 34139
Nfyc
Name: nuclear transcription factor-Y gamma
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18046
HGNC: HGNC:7806
Homologene: 7440
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 160,122,827 bp
  • A to T, chromosome 2 at 34,581,290 bp
  • A to C, chromosome 2 at 52,112,551 bp
  • A to G, chromosome 2 at 85,648,103 bp
  • A to G, chromosome 2 at 97,629,632 bp
  • G to A, chromosome 2 at 166,921,973 bp
  • A to T, chromosome 2 at 180,673,229 bp
  • A to G, chromosome 3 at 79,512,555 bp
  • T to C, chromosome 3 at 96,163,734 bp
  • T to A, chromosome 3 at 108,398,902 bp
  • C to T, chromosome 4 at 33,018,734 bp
  • A to G, chromosome 4 at 118,830,015 bp
  • A to T, chromosome 4 at 120,768,892 bp
  • T to C, chromosome 4 at 152,524,290 bp
  • C to T, chromosome 5 at 9,120,428 bp
  • C to A, chromosome 5 at 37,284,158 bp
  • T to A, chromosome 5 at 53,883,689 bp
  • T to C, chromosome 5 at 110,544,751 bp
  • T to A, chromosome 5 at 114,897,791 bp
  • T to C, chromosome 5 at 149,215,194 bp
  • G to T, chromosome 6 at 17,491,747 bp
  • C to T, chromosome 6 at 46,759,773 bp
  • A to G, chromosome 6 at 77,758,590 bp
  • T to C, chromosome 6 at 125,712,160 bp
  • G to A, chromosome 7 at 110,039,245 bp
  • C to T, chromosome 8 at 5,098,443 bp
  • A to T, chromosome 8 at 12,842,932 bp
  • T to A, chromosome 9 at 78,667,346 bp
  • T to C, chromosome 9 at 107,526,397 bp
  • T to C, chromosome 10 at 77,081,154 bp
  • T to C, chromosome 10 at 79,827,916 bp
  • A to T, chromosome 10 at 130,446,866 bp
  • T to C, chromosome 11 at 49,216,492 bp
  • G to A, chromosome 11 at 69,232,963 bp
  • C to A, chromosome 12 at 8,985,985 bp
  • T to A, chromosome 12 at 13,220,748 bp
  • T to C, chromosome 12 at 78,849,746 bp
  • C to T, chromosome 13 at 49,237,476 bp
  • G to T, chromosome 13 at 53,117,364 bp
  • G to A, chromosome 13 at 58,191,839 bp
  • T to A, chromosome 13 at 63,824,815 bp
  • A to G, chromosome 13 at 95,442,124 bp
  • T to C, chromosome 14 at 32,526,406 bp
  • T to C, chromosome 14 at 43,714,763 bp
  • T to C, chromosome 15 at 27,901,200 bp
  • A to G, chromosome 15 at 75,217,777 bp
  • G to A, chromosome 15 at 83,227,860 bp
  • G to T, chromosome 15 at 86,033,085 bp
  • T to C, chromosome 16 at 17,910,941 bp
  • C to T, chromosome 16 at 32,753,508 bp
  • T to C, chromosome 16 at 49,701,900 bp
  • G to T, chromosome 16 at 62,907,739 bp
  • G to A, chromosome 17 at 36,084,219 bp
  • C to T, chromosome 18 at 67,448,916 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8479 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067923-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.