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Strain Name:
C57BL/6J-MtgxR8493Btlr/Mmmh
Stock Number:
067935-MU
Citation ID:
RRID:MMRRC_067935-MU
Other Names:
R8493 (G1)
Major Collection:

Strain Information

Kif3a
Name: kinesin family member 3A
Synonyms: kinesin-II subunit, Kns3, Kif3, Kifl, N-4 kinesin
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16568
HGNC: HGNC:6319
Homologene: 38266
Smarca4
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Synonyms: SNF2beta, Brg1, SW1/SNF, b2b692Clo, b2b508.1Clo
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20586
Homologene: 135927
Mthfd1
Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase
Synonyms: Mthfd, DCS, E430024A07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 108156
VEGA: 12
HGNC: HGNC:7432
Homologene: 55940
Mtmr7
Name: myotubularin related protein 7
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54384
HGNC: HGNC:7454
Homologene: 99732
Jade1
Name: jade family PHD finger 1
Synonyms: D530048A03Rik, Phf17
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 269424
Homologene: 18162
Nucks1
Name: nuclear casein kinase and cyclin-dependent kinase substrate 1
Synonyms: Nucks, 2700010L10Rik, 8430423A01Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98415
Homologene: 23377
Fam169a
Name: family with sequence similarity 169, member A
Synonyms: B230112C05Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 320557
VEGA: 13
Homologene: 52656
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 45,911,416 bp
  • T to C, chromosome 1 at 53,472,908 bp
  • A to C, chromosome 1 at 87,778,982 bp
  • A to G, chromosome 1 at 91,378,544 bp
  • A to T, chromosome 1 at 131,929,735 bp
  • T to C, chromosome 1 at 171,241,108 bp
  • A to T, chromosome 1 at 185,407,174 bp
  • A to G, chromosome 2 at 26,472,239 bp
  • A to G, chromosome 2 at 32,779,740 bp
  • C to T, chromosome 2 at 62,850,659 bp
  • T to A, chromosome 2 at 87,252,871 bp
  • T to A, chromosome 2 at 87,544,770 bp
  • T to A, chromosome 2 at 89,752,255 bp
  • T to A, chromosome 2 at 111,564,979 bp
  • T to A, chromosome 3 at 41,604,678 bp
  • T to A, chromosome 3 at 51,484,374 bp
  • A to G, chromosome 3 at 88,058,450 bp
  • T to C, chromosome 3 at 144,912,150 bp
  • C to T, chromosome 4 at 118,690,032 bp
  • T to C, chromosome 5 at 11,921,522 bp
  • G to A, chromosome 5 at 34,348,691 bp
  • C to T, chromosome 5 at 95,340,233 bp
  • A to G, chromosome 5 at 103,564,165 bp
  • A to G, chromosome 5 at 103,569,805 bp
  • A to G, chromosome 5 at 122,261,489 bp
  • C to T, chromosome 6 at 32,215,712 bp
  • G to A, chromosome 6 at 40,949,347 bp
  • A to T, chromosome 6 at 47,796,641 bp
  • T to A, chromosome 6 at 115,910,331 bp
  • A to T, chromosome 7 at 45,635,043 bp
  • A to T, chromosome 7 at 48,447,573 bp
  • G to A, chromosome 7 at 101,386,518 bp
  • A to T, chromosome 7 at 103,538,272 bp
  • G to T, chromosome 7 at 104,670,815 bp
  • A to G, chromosome 7 at 105,898,881 bp
  • C to A, chromosome 8 at 11,628,518 bp
  • G to A, chromosome 8 at 40,606,884 bp
  • C to T, chromosome 8 at 70,836,857 bp
  • T to A, chromosome 8 at 94,523,220 bp
  • A to G, chromosome 8 at 110,030,463 bp
  • T to G, chromosome 8 at 110,962,221 bp
  • A to G, chromosome 8 at 124,903,194 bp
  • G to A, chromosome 9 at 21,658,848 bp
  • G to A, chromosome 9 at 44,076,053 bp
  • T to C, chromosome 9 at 65,346,757 bp
  • G to A, chromosome 9 at 78,657,519 bp
  • A to G, chromosome 9 at 107,996,254 bp
  • A to G, chromosome 9 at 111,468,147 bp
  • G to T, chromosome 9 at 122,750,508 bp
  • C to T, chromosome 10 at 76,403,623 bp
  • T to A, chromosome 11 at 9,510,668 bp
  • C to T, chromosome 11 at 22,285,842 bp
  • A to T, chromosome 11 at 53,598,800 bp
  • T to A, chromosome 11 at 69,452,978 bp
  • T to G, chromosome 11 at 119,333,609 bp
  • A to T, chromosome 12 at 8,009,009 bp
  • A to C, chromosome 12 at 40,036,473 bp
  • A to G, chromosome 12 at 76,294,155 bp
  • A to T, chromosome 12 at 91,813,961 bp
  • G to T, chromosome 13 at 97,122,859 bp
  • G to T, chromosome 13 at 116,305,299 bp
  • T to C, chromosome 14 at 4,398,567 bp
  • T to A, chromosome 14 at 7,869,822 bp
  • A to T, chromosome 14 at 55,700,297 bp
  • G to T, chromosome 14 at 56,914,149 bp
  • C to T, chromosome 15 at 76,002,653 bp
  • C to T, chromosome 15 at 78,994,126 bp
  • C to A, chromosome 15 at 85,938,006 bp
  • T to A, chromosome 15 at 102,358,402 bp
  • A to T, chromosome 16 at 4,835,589 bp
  • A to G, chromosome 17 at 19,393,758 bp
  • G to T, chromosome 17 at 26,121,957 bp
  • A to G, chromosome 17 at 34,059,962 bp
  • A to T, chromosome 17 at 39,539,930 bp
  • G to A, chromosome 17 at 68,630,244 bp
  • G to A, chromosome 17 at 74,743,052 bp
  • C to G, chromosome 18 at 61,114,882 bp
  • C to T, chromosome 18 at 61,507,206 bp
  • G to A, chromosome 19 at 46,652,549 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8493 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067935-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.