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Strain Name:
C57BL/6J-MtgxR8500Btlr/Mmmh
Stock Number:
067942-MU
Citation ID:
RRID:MMRRC_067942-MU
Other Names:
R8500 (G1)
Major Collection:

Strain Information

Hlcs
Name: holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase)
Synonyms: 410I21.SP6, D16Jhu34
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 110948
VEGA: 16
HGNC: HGNC:4976
Homologene: 37302
Slc1a3
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 3
Synonyms: MGluT1, Eaat1, B430115D02Rik, GLAST, Gmt1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20512
Homologene: 20882
Gria2
Name: glutamate receptor, ionotropic, AMPA2 (alpha 2)
Synonyms: GluR-B, GluR2, Glur-2, Glur2, GluA2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14800
HGNC: HGNC:4572
Homologene: 20225
Scarb1
Name: scavenger receptor class B, member 1
Synonyms: D5Ertd460e, Srb1, Cd36l1, Cla-1, SRBI, SR-BI, Hlb398, Hdlq1, Chohd1, Chohd1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20778
HGNC: HGNC:1664
Homologene: 21132
Blm
Name: Bloom syndrome, RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12144
HGNC: HGNC:1058
Homologene: 47902
Pard3
Name: par-3 family cell polarity regulator
Synonyms: ASIP, D8Ertd580e, PAR-3, Par3, Pard3a
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 93742
Homologene: 10489
Schip1
Name: schwannomin interacting protein 1
Synonyms: SCHIP-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 30953
Homologene: 130673
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 4,346,590 bp
  • A to T, chromosome 1 at 166,674,470 bp
  • T to C, chromosome 1 at 173,486,745 bp
  • T to A, chromosome 2 at 30,256,196 bp
  • A to T, chromosome 2 at 30,359,204 bp
  • T to A, chromosome 2 at 41,506,779 bp
  • T to C, chromosome 2 at 86,988,478 bp
  • T to C, chromosome 2 at 153,436,346 bp
  • T to C, chromosome 2 at 164,953,566 bp
  • G to T, chromosome 3 at 68,494,613 bp
  • A to T, chromosome 3 at 80,692,467 bp
  • A to T, chromosome 3 at 93,773,528 bp
  • A to G, chromosome 4 at 34,826,691 bp
  • A to G, chromosome 4 at 48,422,109 bp
  • T to A, chromosome 4 at 126,077,410 bp
  • A to G, chromosome 4 at 139,300,129 bp
  • T to A, chromosome 4 at 155,385,691 bp
  • A to T, chromosome 5 at 9,511,726 bp
  • A to G, chromosome 5 at 125,294,163 bp
  • T to C, chromosome 6 at 121,412,854 bp
  • T to C, chromosome 6 at 130,022,697 bp
  • A to G, chromosome 7 at 80,455,284 bp
  • A to G, chromosome 7 at 85,870,430 bp
  • T to G, chromosome 7 at 101,290,266 bp
  • T to C, chromosome 8 at 36,231,138 bp
  • T to A, chromosome 8 at 117,047,563 bp
  • T to A, chromosome 8 at 127,460,303 bp
  • T to C, chromosome 9 at 21,140,801 bp
  • A to T, chromosome 9 at 49,520,145 bp
  • T to A, chromosome 9 at 79,609,851 bp
  • A to G, chromosome 10 at 103,046,155 bp
  • T to A, chromosome 11 at 4,175,039 bp
  • T to C, chromosome 11 at 60,704,983 bp
  • C to G, chromosome 11 at 73,710,985 bp
  • A to T, chromosome 12 at 79,287,680 bp
  • T to C, chromosome 12 at 113,796,214 bp
  • A to T, chromosome 13 at 48,586,283 bp
  • A to T, chromosome 13 at 67,390,709 bp
  • A to C, chromosome 13 at 100,222,712 bp
  • T to G, chromosome 14 at 52,667,635 bp
  • T to C, chromosome 15 at 8,642,369 bp
  • C to T, chromosome 15 at 84,987,635 bp
  • T to A, chromosome 15 at 100,675,332 bp
  • T to C, chromosome 16 at 45,980,586 bp
  • G to A, chromosome 16 at 73,948,340 bp
  • G to A, chromosome 16 at 94,262,758 bp
  • A to G, chromosome 17 at 12,709,441 bp
  • A to T, chromosome 17 at 27,902,620 bp
  • C to T, chromosome 17 at 56,171,749 bp
  • A to G, chromosome 18 at 34,768,801 bp
  • C to T, chromosome 19 at 33,464,449 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8500 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067942-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.