Strain Name:
C57BL/6J-MtgxR8500Btlr/Mmmh
Stock Number:
067942-MU
Citation ID:
RRID:MMRRC_067942-MU
Other Names:
R8500 (G1)
Major Collection:

Strain Information

Hlcs
Name: holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase)
Synonyms: 410I21.SP6, D16Jhu34
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 110948
VEGA: 16
HGNC: HGNC:4976
Homologene: 37302
Slc1a3
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 3
Synonyms: Eaat1, GLAST, Gmt1, B430115D02Rik, MGluT1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20512
Homologene: 20882
Gria2
Name: glutamate receptor, ionotropic, AMPA2 (alpha 2)
Synonyms: Glur2, GluA2, GluR-B, Glur-2, GluR2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14800
HGNC: HGNC:4572
Homologene: 20225
Scarb1
Name: scavenger receptor class B, member 1
Synonyms: Cla-1, SRBI, Chohd1, SR-BI, Srb1, Chohd1, Cd36l1, D5Ertd460e, Hlb398, Hdlq1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20778
HGNC: HGNC:1664
Homologene: 21132
Blm
Name: Bloom syndrome, RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12144
HGNC: HGNC:1058
Homologene: 47902
Pard3
Name: par-3 family cell polarity regulator
Synonyms: Par3, D8Ertd580e, PAR-3, ASIP, Pard3a
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 93742
Homologene: 10489
Schip1
Name: schwannomin interacting protein 1
Synonyms: SCHIP-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 30953
Homologene: 130673
Zfp292
Name: zinc finger protein 292
Synonyms: 9430062L07Rik, Zfp-15, Krox-10, Zn-15, Zfp15, Zn-16, 5730450D02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 30046
Homologene: 8493
Taf11
Name: TATA-box binding protein associated factor 11
Synonyms: TAF2I, TAFII28, 1110038O14Rik, PRO214, 28kDa
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 68776
Homologene: 55918
Sh3glb2
Name: SH3-domain GRB2-like endophilin B2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227700
Homologene: 10595
Robo2
Name: roundabout guidance receptor 2
Synonyms: 9430089E08Rik, 2600013A04Rik, D230004I22Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 268902
Homologene: 43188
Iqsec3
Name: IQ motif and Sec7 domain 3
Synonyms: synarfGEF, BRAG3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243621
Homologene: 46091
Ncam1
Name: neural cell adhesion molecule 1
Synonyms: NCAM-140, NCAM-180, CD56, NCAM-1, E-NCAM, NCAM-120, NCAM
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17967
HGNC: HGNC:7656
Homologene: 40754
Lrrc8a
Name: leucine rich repeat containing 8A VRAC subunit A
Synonyms: ebo, SWELL1, Lrrc8
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241296
Homologene: 18617
Igf2r
Name: insulin-like growth factor 2 receptor
Synonyms: IGF-II/CI-MPR, CI-MPR, mannose-6-phosphate receptor, cation independent, M6P/IGF2R, Mpr300, CD222
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16004
HGNC: HGNC:5467
Homologene: 676
Cdc37
Name: cell division cycle 37
Synonyms: p50Cdc37
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12539
VEGA: 9
HGNC: HGNC:1735
Homologene: 38268
Zfyve26
Name: zinc finger, FYVE domain containing 26
Synonyms: 9330197E15Rik, A630028O16Rik, LOC380767
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 211978
VEGA: 12
Homologene: 9102
Lonrf1
Name: LON peptidase N-terminal domain and ring finger 1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244421
Homologene: 34980
Plcxd2
Name: phosphatidylinositol-specific phospholipase C, X domain containing 2
Synonyms: EG433022
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 433022
Homologene: 44374
Sf3a1
Name: splicing factor 3a, subunit 1
Synonyms: 5930416L09Rik, 1200014H24Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67465
Homologene: 4294
Invs
Name: inversin
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 16348
Homologene: 7786
Col12a1
Name: collagen, type XII, alpha 1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12816
HGNC: HGNC:2188
Homologene: 3217
Mmp9
Name: matrix metallopeptidase 9
Synonyms: B/MMP9, MMP-9, gelatinase B, Clg4b, Gelatinase B, Gel B
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17395
HGNC: HGNC:7176
Homologene: 3659
Naip5
Name: NLR family, apoptosis inhibitory protein 5
Synonyms: Naip-rs3, Lgn1, Birc1e
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17951
HGNC: HGNC:7634
Homologene: 113589
Zfp429
Name: zinc finger protein 429
Synonyms: 2810487A22Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 72807
Homologene: 110878
Ptpdc1
Name: protein tyrosine phosphatase domain containing 1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218232
VEGA: 13
Homologene: 17576
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Slc66a1
Name: solute carrier family 66 member 1
Synonyms: Pqlc2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 212555
Homologene: 56784
Grm3
Name: glutamate receptor, metabotropic 3
Synonyms: mGlu3, Gprc1c, 0710001G23Rik, mGluR3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 108069
HGNC: HGNC:4595
Homologene: 651
Rp1
Name: retinitis pigmentosa 1 (human)
Synonyms: Dcdc3, mG145, oxygen-regulated protein 1, Orp1, Rp1h
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19888
Homologene: 4564
Lrriq1
Name: leucine-rich repeats and IQ motif containing 1
Synonyms: 4930503E15Rik, LOC380658, Gm1557
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74978
Homologene: 46007
Ifi206
Name: interferon activated gene 206
Synonyms: Gm4955, Pyblhin-C
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 102639543
HGNC: HGNC:5395
Homologene: 115929
Vmn2r73
Name: vomeronasal 2, receptor 73
Synonyms: EG620928
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 620928
Homologene: 115466
Fmo9
Name: flavin containing monooxygenase 9
Synonyms: 4831428F09Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240894
Homologene: 134192
Zfp735
Name: zinc finger protein 735
Synonyms: 1700012C15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76390
Homologene: 88945
Pkd1l2
Name: polycystic kidney disease 1 like 2
Synonyms: 1700126L06Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 76645
Homologene: 124481
Gm5773
Name: predicted pseudogene 5773
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 436563
5031439G07Rik
Name: RIKEN cDNA 5031439G07 gene
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223739
HGNC: HGNC:1314
Homologene: 15140
Llgl1
Name: LLGL1 scribble cell polarity complex component
Synonyms: Lgl1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16897
HGNC: HGNC:6628
Homologene: 31220
Nol4l
Name: nucleolar protein 4-like
Synonyms: 8430427H17Rik, LOC381396
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 329540
Homologene: 129589
Atg16l2
Name: autophagy related 16 like 2
Synonyms: 2410118P20Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 73683
Homologene: 18981
Klra6
Name: killer cell lectin-like receptor, subfamily A, member 6
Synonyms: Ly49f, Ly49F
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16637
Homologene: 110821
Tnfaip8l1
Name: tumor necrosis factor, alpha-induced protein 8-like 1
Synonyms: 2600017J23Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66443
Homologene: 23547
Gabrd
Name: gamma-aminobutyric acid (GABA) A receptor, subunit delta
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14403
HGNC: HGNC:4084
Homologene: 55489
Oscp1
Name: organic solute carrier partner 1
Synonyms: 1810007P19Rik, 6030436A01Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230751
Homologene: 71010
Or5t16
Name: olfactory receptor family 5 subfamily T member 16
Synonyms: Olfr1101, MOR179-3, GA_x6K02T2Q125-48475870-48474938
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258584
Homologene: 74087
Fam53c
Name: family with sequence similarity 53, member C
Synonyms: 2810012G03Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 66306
VEGA: 18
HGNC: HGNC:1336
Homologene: 9586
Lipo5
Name: lipase, member O5
Synonyms: Gm8975
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 668095
Ighv2-6-8
Name: immunoglobulin heavy variable 2-6-8
Synonyms: Gm16887
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 791090
Cela1
Name: chymotrypsin-like elastase family, member 1
Synonyms: Ela1, 1810062B19Rik, PC-TsF, Ela-1, 1810009A17Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 109901
VEGA: 15
HGNC: HGNC:3308
Homologene: 20454
Trav6-2
Name: T cell receptor alpha variable 6-2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 436539
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 4,346,590 bp
  • A to T, chromosome 1 at 166,674,470 bp
  • T to C, chromosome 1 at 173,486,745 bp
  • T to A, chromosome 2 at 30,256,196 bp
  • A to T, chromosome 2 at 30,359,204 bp
  • T to A, chromosome 2 at 41,506,779 bp
  • T to C, chromosome 2 at 86,988,478 bp
  • T to C, chromosome 2 at 153,436,346 bp
  • T to C, chromosome 2 at 164,953,566 bp
  • G to T, chromosome 3 at 68,494,613 bp
  • A to T, chromosome 3 at 80,692,467 bp
  • A to T, chromosome 3 at 93,773,528 bp
  • A to G, chromosome 4 at 34,826,691 bp
  • A to G, chromosome 4 at 48,422,109 bp
  • T to A, chromosome 4 at 126,077,410 bp
  • A to G, chromosome 4 at 139,300,129 bp
  • T to A, chromosome 4 at 155,385,691 bp
  • A to T, chromosome 5 at 9,511,726 bp
  • A to G, chromosome 5 at 125,294,163 bp
  • T to C, chromosome 6 at 121,412,854 bp
  • T to C, chromosome 6 at 130,022,697 bp
  • A to G, chromosome 7 at 80,455,284 bp
  • A to G, chromosome 7 at 85,870,430 bp
  • T to G, chromosome 7 at 101,290,266 bp
  • T to C, chromosome 8 at 36,231,138 bp
  • T to A, chromosome 8 at 117,047,563 bp
  • T to A, chromosome 8 at 127,460,303 bp
  • T to C, chromosome 9 at 21,140,801 bp
  • A to T, chromosome 9 at 49,520,145 bp
  • T to A, chromosome 9 at 79,609,851 bp
  • A to G, chromosome 10 at 103,046,155 bp
  • T to A, chromosome 11 at 4,175,039 bp
  • T to C, chromosome 11 at 60,704,983 bp
  • C to G, chromosome 11 at 73,710,985 bp
  • A to T, chromosome 12 at 79,287,680 bp
  • T to C, chromosome 12 at 113,796,214 bp
  • A to T, chromosome 13 at 48,586,283 bp
  • A to T, chromosome 13 at 67,390,709 bp
  • A to C, chromosome 13 at 100,222,712 bp
  • T to G, chromosome 14 at 52,667,635 bp
  • T to C, chromosome 15 at 8,642,369 bp
  • C to T, chromosome 15 at 84,987,635 bp
  • T to A, chromosome 15 at 100,675,332 bp
  • T to C, chromosome 16 at 45,980,586 bp
  • G to A, chromosome 16 at 73,948,340 bp
  • G to A, chromosome 16 at 94,262,758 bp
  • A to G, chromosome 17 at 12,709,441 bp
  • A to T, chromosome 17 at 27,902,620 bp
  • C to T, chromosome 17 at 56,171,749 bp
  • A to G, chromosome 18 at 34,768,801 bp
  • C to T, chromosome 19 at 33,464,449 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8500 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067942-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.