Strain Name:
C57BL/6J-MtgxR8527Btlr/Mmmh
Stock Number:
067952-MU
Citation ID:
RRID:MMRRC_067952-MU
Other Names:
R8527 (G1)
Major Collection:

Strain Information

Eya1
Name: EYA transcriptional coactivator and phosphatase 1
Synonyms: bor
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14048
HGNC: HGNC:3519
Homologene: 74943
Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4833417A11Rik, 4832428G11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441
Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: Nav1.3, LOC381367
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20269
Homologene: 56005
Tjp2
Name: tight junction protein 2
Synonyms: ZO-2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21873
VEGA: 19
Homologene: 3541
Kdm5b
Name: lysine demethylase 5B
Synonyms: Plu1, 2010009J12Rik, D1Ertd202e, Rb-Bp2, Jarid1b, 2210016I17Rik, PLU-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 75605
Homologene: 48448
Arhgef12
Name: Rho guanine nucleotide exchange factor 12
Synonyms: 2310014B11Rik, LARG
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69632
VEGA: 9
Homologene: 9088
Trpc4ap
Name: transient receptor potential cation channel, subfamily C, member 4 associated protein
Synonyms: Trp4-associated protein TAP1, Trrp4ap, 4833429F06Rik, D2Ertd113e
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56407
Homologene: 9224
Sorbs1
Name: sorbin and SH3 domain containing 1
Synonyms: 9530001P15Rik, Sh3d5, Ponsin, c-Cbl-associated protein, CAP, 2310065E01Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20411
VEGA: 19
Homologene: 83252
Braf
Name: Braf transforming gene
Synonyms: Braf-2, Braf2, 9930012E13Rik, D6Ertd631e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 109880
HGNC: HGNC:1097
Homologene: 3197
Smc4
Name: structural maintenance of chromosomes 4
Synonyms: Smc4l1, 2500002A22Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 70099
Homologene: 4015
Tanc2
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 2
Synonyms: 3526402J09Rik, 5730590C14Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 77097
Homologene: 64680
Rasgrp1
Name: RAS guanyl releasing protein 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19419
HGNC: HGNC:9878
Homologene: 4195
Kctd2
Name: potassium channel tetramerisation domain containing 2
Synonyms: 2310012I15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 70382
Homologene: 82389
Acsl5
Name: acyl-CoA synthetase long-chain family member 5
Synonyms: 1700030F05Rik, Facl5
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 433256
VEGA: 19
Homologene: 69208
Olfm3
Name: olfactomedin 3
Synonyms: optimedin, B230206G02Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229759
Homologene: 17103
Atf7ip2
Name: activating transcription factor 7 interacting protein 2
Synonyms: Get-1, PSM2, 4930558K11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 75329
Homologene: 23509
Lonrf1
Name: LON peptidase N-terminal domain and ring finger 1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244421
Homologene: 34980
Slc29a3
Name: solute carrier family 29 (nucleoside transporters), member 3
Synonyms: Ent3, 4933435C21Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 71279
Homologene: 56805
Gipc3
Name: GIPC PDZ domain containing family, member 3
Synonyms: Rgs19ip3, Gipc3, Ahl5
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 209047
Homologene: 77068
Ankrd35
Name: ankyrin repeat domain 35
Synonyms: 4732436F15Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 213121
Homologene: 16977
Myh11
Name: myosin, heavy polypeptide 11, smooth muscle
Synonyms: SM2, smMHC, SM1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 17880
VEGA: 16
HGNC: HGNC:7569
Homologene: 128512
Tmem209
Name: transmembrane protein 209
Synonyms: 2700094F01Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 72649
Homologene: 18021
Rb1cc1
Name: RB1-inducible coiled-coil 1
Synonyms: Cc1, LaXp180, 5930404L04Rik, Fip200, 2900055E04Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12421
Homologene: 7659
Megf10
Name: multiple EGF-like-domains 10
Synonyms: LOC240312, 3000002B06Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 70417
Homologene: 23771
Gas6
Name: growth arrest specific 6
Synonyms: Gas-6, growth arrest-specific, GAS 6
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14456
HGNC: HGNC:4168
Homologene: 638
Zan
Name: zonadhesin
Synonyms: Zan
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22635
Homologene: 124417
Tnxb
Name: tenascin XB
Synonyms: Tnx, TN-MHC
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 81877
Homologene: 49589
Mtus2
Name: microtubule associated tumor suppressor candidate 2
Synonyms: 5730592G18Rik, A730013O20Rik, C130038G02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77521
Homologene: 78141
Kif14
Name: kinesin family member 14
Synonyms: D1Ertd367e, N-3 kinesin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381293
Homologene: 8916
Traf3ip3
Name: TRAF3 interacting protein 3
Synonyms: 6030423D04Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 215243
Homologene: 11885
Smarca2
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Synonyms: brm, 2610209L14Rik, Snf2l2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 67155
Homologene: 2308
Efcab12
Name: EF-hand calcium binding domain 12
Synonyms: BC060267
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 212516
Homologene: 18905
Fbxl12
Name: F-box and leucine-rich repeat protein 12
Synonyms: 3110048D16Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 30843
Homologene: 32198
Tex52
Name: testis expressed 52
Synonyms: 4933413G19Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 71149
Homologene: 130761
Pax5
Name: paired box 5
Synonyms: EBB-1, Pax-5
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18507
HGNC: HGNC:8619
Homologene: 56419
Lca5l
Name: Leber congenital amaurosis 5-like
Synonyms: 4921526F01Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 385668
HGNC: HGNC:1255
Homologene: 47978
Vav3
Name: vav 3 oncogene
Synonyms: A530094I06Rik, Idd18.1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 57257
Homologene: 38143
Lmod1
Name: leiomodin 1 (smooth muscle)
Synonyms: 9530015K06Rik, SM-Lmod, D1, 1D, 64kD D1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 93689
HGNC: HGNC:6647
Homologene: 8118
Mmp24
Name: matrix metallopeptidase 24
Synonyms: MT5-MMP, Membrane type 5-MMP
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17391
HGNC: HGNC:7172
Homologene: 21331
Gm12689
Name: predicted gene 12689
Type: Gene
Species: Mouse
Chromosome: 4
Apmap
Name: adipocyte plasma membrane associated protein
Synonyms: 2310001A20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71881
Homologene: 41380
Rpl3l
Name: ribosomal protein L3-like
Synonyms: 1110057H16Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66211
Homologene: 68434
Tmem121b
Name: transmembrane protein 121B
Synonyms: Cecr6
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 94047
HGNC: HGNC:1844
Homologene: 12893
Dgcr6
Name: DiGeorge syndrome critical region gene 6
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13353
Homologene: 4152
Noc2l
Name: NOC2 like nucleolar associated transcriptional repressor
Synonyms: NIR
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 57741
VEGA: 4
Homologene: 6980
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 6,244,875 bp
  • G to T, chromosome 1 at 14,252,448 bp
  • C to T, chromosome 1 at 71,309,888 bp
  • A to T, chromosome 1 at 134,605,774 bp
  • T to C, chromosome 1 at 135,364,483 bp
  • T to A, chromosome 1 at 136,468,757 bp
  • T to A, chromosome 1 at 193,194,543 bp
  • G to T, chromosome 2 at 65,497,519 bp
  • A to T, chromosome 2 at 117,338,304 bp
  • T to C, chromosome 2 at 150,586,465 bp
  • G to C, chromosome 2 at 155,692,212 bp
  • A to G, chromosome 2 at 155,799,714 bp
  • T to A, chromosome 3 at 69,032,891 bp
  • A to G, chromosome 3 at 96,682,037 bp
  • A to G, chromosome 3 at 109,503,471 bp
  • A to T, chromosome 3 at 115,122,547 bp
  • A to T, chromosome 4 at 44,570,071 bp
  • A to G, chromosome 4 at 99,296,213 bp
  • A to G, chromosome 4 at 156,241,730 bp
  • T to C, chromosome 5 at 137,435,709 bp
  • A to C, chromosome 5 at 148,303,598 bp
  • C to A, chromosome 6 at 30,497,238 bp
  • T to C, chromosome 6 at 39,627,759 bp
  • T to C, chromosome 6 at 115,814,574 bp
  • T to C, chromosome 6 at 120,492,094 bp
  • G to A, chromosome 6 at 128,379,865 bp
  • T to A, chromosome 7 at 25,538,730 bp
  • T to C, chromosome 8 at 13,465,790 bp
  • G to T, chromosome 8 at 36,219,832 bp
  • C to A, chromosome 9 at 20,638,864 bp
  • A to T, chromosome 9 at 42,997,648 bp
  • A to G, chromosome 10 at 60,730,622 bp
  • T to A, chromosome 10 at 81,338,221 bp
  • A to G, chromosome 11 at 105,917,008 bp
  • G to T, chromosome 11 at 115,429,484 bp
  • T to A, chromosome 16 at 10,237,265 bp
  • T to C, chromosome 16 at 14,230,706 bp
  • T to C, chromosome 16 at 18,069,526 bp
  • C to A, chromosome 16 at 96,159,238 bp
  • A to G, chromosome 17 at 24,735,780 bp
  • A to G, chromosome 17 at 34,688,660 bp
  • T to C, chromosome 18 at 57,292,718 bp
  • C to A, chromosome 19 at 24,111,573 bp
  • G to C, chromosome 19 at 26,676,787 bp
  • G to C, chromosome 19 at 40,376,800 bp
  • A to G, chromosome 19 at 55,291,827 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8527 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067952-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.