Strain Name:
C.Cg-Msx1tm1Rilm/Mmucd
Stock Number:
000068-UCD
Citation ID:
RRID:MMRRC_000068-UCD
Other Names:
C.Cg-Msx1tm1Rilm, C.Cg-Msx1tm1Rilm/Mmcd

Strain Information

Msx1tm1Rilm
Name: msh homeobox 1; targeted mutation 1, Richard Maas
Synonyms: Msx1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Knockout
Msx1
Name: msh homeobox 1
Synonyms: Hox7, Hox7.1, muscle-segment homeobox, msh, Hox-7
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Knockout
NCBI: 17701
HGNC: HGNC:7391
Homologene: 1836
Genetic Alterations
A neo cassette was inserted into exon 2.
Genotype Determination
ES Cell Line
J1 derived from 129S4/SvJae
Phenotype
All Msx1 -/- newborn mice die within 24 hours of birth. All Msx1 -/- manifest a cleft secondary palate, a deficiency of alveolar mandible and maxilla, and a failure of tooth development. These mice also exhibit abnormalities of the nasal, frontal, and parietal bones, and of the malleus in the middle ear.
Mammalian Phenotype Terms
Allelic Composition: Msx1tm1Rilm/Msx1tm1Rilm (Genetic Background: either: (involves: 129S4/SvJae-Msx1tm1Rilm) or (involves: 129S4/SvJae * BALB/c) or (involves: 129X1/SvJae * C57BL/6J) )

Allelic Composition: Msx1tm1Rilm/Msx1tm1Rilm (Genetic Background: involves: 129S4/SvJae )

MeSH Terms
  • Abnormalities, Multiple/embryology
  • Abnormalities, Multiple/genetics
  • Animals
  • Base Sequence
  • Cell Line
  • Chimera
  • Cleft Palate/embryology
  • Cleft Palate/genetics
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/physiology
  • Dental Papilla/abnormalities
  • Dental Papilla/embryology
  • Disease Models, Animal
  • Embryo Transfer
  • Embryonic Induction/genetics
  • Facial Bones/abnormalities
  • Facial Bones/embryology
  • Female
  • Genes, Homeobox
  • Genes, Lethal
  • Genes, Recessive
  • Head/embryology
  • Homeodomain Proteins
  • Humans
  • Jaw Abnormalities/embryology
  • Jaw Abnormalities/genetics
  • MSX1 Transcription Factor
  • Male
  • Malleus/abnormalities
  • Malleus/embryology
  • Mesoderm/pathology
  • Mice
  • Mice, Mutant Strains/embryology
  • Mice, Mutant Strains/genetics
  • Molecular Sequence Data
  • Morphogenesis/genetics
  • Phenotype
  • Skull/abnormalities
  • Skull/embryology
  • Tooth Abnormalities/embryology
  • Tooth Abnormalities/genetics
  • Transcription Factors
Strain Development
BALB/c blastocysts injected with ES cells were transferred into pseudopregnant recipients. Male chimaeras, identified by agouti coat color, were mated with BALB/cTac females.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • This mutant serves as a genetic model for cleft palate and oligodontia.
Donor
Richard Maas, MD. PhD. Brigham and Women's Hospital / Harvard Medical School
Primary Reference
Satokata I, Maas R. Msx1 deficient mice exhibit cleft palate and abnormalities of cranialfacial and tooth development. Nature Genetics. Apr 1994, v6, p348. (Medline PMID: 7914451)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
White
MMRRC Breeding System
Sib mating
Breeding Scheme(s)
  • Heterozygous female x Sibling wildtype male
  • Wildtype female x Sibling heterozygous male
Generation
N10 to Balb/cTac F2 As of: 4/26/2004
Overall Breeding Performance
Good
Average litter size
8
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000068-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000068-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
000068-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.