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Availability & Fees Order this Strain
H-2DM (encoded by the H2-DMa gene) is a peptide editor involved in the loading immunogenic peptides onto MHC Class II molecules. When the H2-DMa gene is deleted in antigen-presenting cells (APCs, i.e., dendritic cells, macrophages, B cells), the APCs are deficient in antigen processing and presentation. Under SPF conditions the mice appear to be normal in their behavior, breeding, and lifespan.
Conditional phenotype: This conditional allele allows elimination of antigen-presenting capacity in distinct antigen-presenting cell subsets using a variant of Cre deleter strains, whereas the germline H2-DMa knockout has deficient antigen processing and presentation in all APC types.
This strain is a very valuable tool to dissect antigen-presenting cells in vivo. The conditional knockout allele of MHC class II molecules has issues with germline deletions when crossed to various Cre strains; complete loss of class II molecules can cause unwanted side effects on the immune system. This line (also known as "H-2DM f/f") allows for elimination of the antigen processing and presentation capacity of an APC subset while retaining the expression of class II molecules. This is particularly useful to examine dendritic cell subsets and their function.
Bartleson JM, Viehmann Milam AA, Donermeyer DL, Horvath S, Xia Y, Egawa T, Allen PM. Strength of tonic T cell receptor signaling instructs T follicular helper cell-fate decisions. Nat Immunol. 2020 Nov;21(11):1384-1396. doi: 10.1038/s41590-020-0781-7. Epub 2020 Sep 28. (Medline PMID: 32989327)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Office of Technology Management.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Office of Technology Management
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.